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    Competitive homogeneous Assay
    43.
    发明公开
    Competitive homogeneous Assay 失效
    竞争性均衡测定

    公开(公告)号：EP0232967A3

    公开(公告)日：1989-05-24

    申请号：EP87300195.2

    申请日：1987-01-09

    申请人： AMOCO CORPORATION

    发明人： Morrison, Larry Edward

    IPC分类号： C12Q1/68 ,  G01N33/58 ,  C12Q1/48

    CPC分类号： C12Q1/6818 ,  C07H21/00 ,  G01N33/582 ,  C12Q2565/525 ,  C12Q2565/107 ,  C12Q2537/161

    摘要： Methods and compositions for performing assays for target polynucleotide strands include contacting a sample with a reagent which includes a first and a second poly­nucleotide probe. The first and second probes are capable of assuming a first position wherein the probes are bound to each other and a second position wherein the probes are bound to a target. The probes include label moieties capable of interacting to produce a signal indi­cative of the probes being in one of the two positions.

    PROBE: ANTIPROBE COMPOSITIONS FOR HIGH SPECIFICITY DNA OR RNA DETECTION
    44.
    发明授权
    PROBE: ANTIPROBE COMPOSITIONS FOR HIGH SPECIFICITY DNA OR RNA DETECTION 有权

    公开(公告)号：EP2756101B1

    公开(公告)日：2018-05-23

    申请号：EP12831521.5

    申请日：2012-09-14

    申请人： Shafer, David, A.

    发明人： Shafer, David, A.

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  C12N15/11

    CPC分类号： C12Q1/6876 ,  C12Q1/6832 ,  C12Q2537/1373 ,  C12Q2537/161 ,  C12Q2537/163

    摘要： Probe systems and methods are provided for detecting nucleic acid targets using labeled polynucleotide probes and antiprobes that interact together and with complementary targets. These interactions result in signaling changes that indicate target frequency and provide error-checking functions that facilitate single base discrimination. These probe:antiprobe compositions enable real-time PCR detection, end-point detection and microarray detection of microbial species, drug resistant mutants, and cancer related variants. The probe:antiprobe may be an internal probe between two primers or may be a primer-probe. The probe also may be modified by introducing a base mismatch to increase thermodynamic discrimination of a correct versus incorrect target differing by a single base. Probe systems also are provided for use in methods of increasing target amplification and detecting specific single base variants.

    METHODS, KITS & COMPOSITIONS FOR DETERMINING GENE COPY NUMBERS
    46.
    发明公开
    METHODS, KITS & COMPOSITIONS FOR DETERMINING GENE COPY NUMBERS 审中-公开
    用于确定基因拷贝数的方法，试剂盒和组合物

    公开(公告)号：EP3207156A1

    公开(公告)日：2017-08-23

    申请号：EP15790334.5

    申请日：2015-10-13

    申请人： Life Technologies Corporation

    发明人： LIU, Chunmei ,  DONG, Shoulian ,  STEVENS, Junko

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2537/16 ,  C12Q2537/161 ,  C12Q2545/107 ,  C12Q2545/113 ,  C12Q2600/16

    摘要： The present specification relates to methods, compositions, and kits for determining the presence of the number of copies of a gene. The present disclosure provides methods and compositions to determine zygosity in transgenic plants, number of transgenes in a plant or animal, karyotyping, determine CNV in human and animal samples, diagnose and detect diseases and conditions that are characterized by having variations in the number of copies of one or more genes.

    摘要翻译： 本说明书涉及用于确定基因拷贝数的存在的方法，组合物和试剂盒。 本公开内容提供了用于确定转基因植物中的接合性，植物或动物中转基因的数量，核型分析，确定人和动物样品中的CNV，诊断和检测特征在于具有拷贝数量变化的疾病和病症的方法和组合物 的一个或多个基因。

    PROBE: ANTIPROBE COMPOSITIONS FOR HIGH SPECIFICITY DNA OR RNA DETECTION
    49.
    发明公开
    PROBE: ANTIPROBE COMPOSITIONS FOR HIGH SPECIFICITY DNA OR RNA DETECTION 有权
    PROBE：ANTIPROBE-ZUSAMMENSETZUNGEN ZUM NACHWEIS HOCHSPEZIFISCHER DNA ODER RNA

    公开(公告)号：EP2756101A4

    公开(公告)日：2015-09-30

    申请号：EP12831521

    申请日：2012-09-14

    申请人： SHAFER DAVID A

    发明人： SHAFER DAVID A

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  C12N15/11

    CPC分类号： C12Q1/6876 ,  C12Q1/6832 ,  C12Q2537/1373 ,  C12Q2537/161 ,  C12Q2537/163

    摘要： Probe systems and methods are provided for detecting nucleic acid targets using labeled polynucleotide probes and antiprobes that interact together and with complementary targets. These interactions result in signaling changes that indicate target frequency and provide error-checking functions that facilitate single base discrimination. These probe:antiprobe compositions enable real-time PCR detection, end-point detection and microarray detection of microbial species, drug resistant mutants, and cancer related variants. The probe:antiprobe may be an internal probe between two primers or may be a primer-probe. The probe also may be modified by introducing a base mismatch to increase thermodynamic discrimination of a correct versus incorrect target differing by a single base. Probe systems also are provided for use in methods of increasing target amplification and detecting specific single base variants.

    摘要翻译： 提供了探针系统和方法，用于使用标记的多核苷酸探针和与辅助靶相互作用的抗体来检测核酸靶。 这些相互作用导致指示目标频率的信号变化，并提供有助于单基地辨别的错误检查功能。 这些探针：反穿刺组合物可实现微生物物种，耐药突变体和癌症相关变异体的实时PCR检测，终点检测和微阵列检测。 探针：反接头可以是两个引物之间的内部探针，也可以是引物探针。 探针也可以通过引入碱基不匹配来增加对单个碱基不同的正确对不正确目标的热力学鉴别。 提供探针系统用于增加靶扩增和检测特异性单碱基变体的方法。

    Methods for non-invasive pre-natal ploidy calling
    50.
    发明公开
    Methods for non-invasive pre-natal ploidy calling 审中-公开
    非侵入性产前倍性呼叫的方法

    公开(公告)号：EP2854058A2

    公开(公告)日：2015-04-01

    申请号：EP14198478.1

    申请日：2011-05-18

    申请人： Natera, Inc.

    发明人： Rabinowitz, Matthew ,  Gemelos, George ,  Banjevic, Milena ,  Ryan, Allison ,  Demko, Zachary ,  Hill, Matthew ,  Zimmermann, Bernhard ,  Baner, Johan

    IPC分类号： G06F19/18 ,  G06F19/22 ,  C12Q1/68

    CPC分类号： C12Q1/6883 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q2537/161 ,  C12Q2537/165 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2600/172 ,  G06F19/00 ,  G06F19/12 ,  G06F19/18 ,  G06F19/22 ,  G06F19/34 ,  G16H50/30

    摘要： Methods for non-invasive prenatal ploidy calling are disclosed herein. Methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a sample of DNA from the mother of the fetus and from the fetus, and from genotypic data from the mother and optionally also from the father are disclosed herein. The ploidy state is determined by using a joint distribution model to create a set of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. In an embodiment, the mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias.

    摘要翻译： 本文公开了非侵入性产前倍性调用的方法。 本文公开了根据从胎儿和胎儿的DNA样品测量的基因型数据以及从母亲和可选地来自父亲的基因型数据测定妊娠胎儿中染色体倍性状态的方法。 通过使用联合分布模型来确定倍性状态，以在给定亲本基因型数据的情况下针对不同可能的胎儿倍性状态创建一组预期的等位基因分布，并且将预期的等位基因分布与在混合样品中测量的测量的等位基因分布的模式进行比较， 并选择其预期等位基因分布模式与观察到的等位基因分布模式最接近匹配的倍性状态。 在一个实施方案中，DNA的混合样品可以以使等位基因偏倚最小化的方式在多个多态性基因座上优先富集。