公开(公告)号：EP0989190A1

公开(公告)日：2000-03-29

申请号：EP98117920.3

申请日：1998-09-22

申请人： FUJI PHOTO FILM CO., LTD.

发明人： Tanaka, Hideaki c/o Fuji Photo Film Co., Ltd., ,  Abe, Yoshihiko c/o Fuji Photo Film Co., Ltd., ,  Terashima, Kaoru c/o Fuji Photo Film Co., Ltd.,

IPC分类号： C12Q1/50 ,  G01N33/52

CPC分类号： C12Q1/50

摘要： A dry analytical element for quantitatively analyzing creatine kinase MB isozyme is composed of a support and a creatine kinase MB isozyme-detecting agent layer. The detecting layer contains an antibody which specifically inhibits activity of M sub-unit of creatine kinase, creatine phosphate and adenosine diphosphate which react with each other to give adenosine triphosphate in the presence of creatine kinase MB isozyme, a buffer compound which keeps the layer in the range of pH 5.5 to pH 8.5 for the formation of adenosine triphosphate, and an indicator composition which reacts with the formed adenosine triphosphate to give a spectroscopically detectable compound, said indicator composition having hexokinase, nicotinamide-adenine dinucleotide or its phosphate in an oxidized form, and glucose-6-phosphate dehydrogenase but not containing glucose in such amount as to give the spectroscopically detectable compound.

摘要翻译： 用于定量分析肌酸激酶MB同功酶的干燥分析元件由载体和肌酸激酶MB同工酶检测剂层组成。 检测层含有特异性抑制肌酸激酶，肌酸磷酸肌酸和二磷酸腺苷的M亚单位的活性的抗体，其在肌酸激酶MB同功酶（一种保持该层的缓冲化合物）存在下相互反应，产生三磷酸腺苷 用于形成三磷酸腺苷的pH5.5至pH8.5的范围以及与所形成的三磷酸腺苷反应以产生光谱可检测化合物的指示剂组合物，所述指示剂组合物具有己糖激酶，烟酰胺 - 腺嘌呤二核苷酸或其氧化形式的磷酸酯 和葡萄糖-6-磷酸脱氢酶，但不含葡萄糖，其量使得可得到光谱可检测的化合物。

公开(公告)号：EP0774514B1

公开(公告)日：2000-03-08

申请号：EP96308273.0

申请日：1996-11-15

申请人： KYOWA MEDEX CO., LTD.

发明人： Aoyama, Norihito ,  Sakakibara, Minako

IPC分类号： C12Q1/50

CPC分类号： C12Q1/50

公开(公告)号：EP0924302A3

公开(公告)日：2000-02-23

申请号：EP98123510.4

申请日：1998-12-15

申请人： UNITIKA LTD.

发明人： Iwata, Ken, Unitika Ltd ,  Kawahara, Kazue Unitika Ltd ,  Nakajima, Hiroshi Unitika Ltd ,  Kondo, Hitoshi Unitika Ltd

IPC分类号： C12Q1/50 ,  C12Q1/32 ,  C12Q1/48

CPC分类号： C12Q1/50 ,  C12Q1/48 ,  Y10S435/97

摘要： Test strips by which creatine kinase activity can be quantitatively measured at a high sensitivity within a broad measuring range and which have excellent storage stability. Particularly, test strips for the measurement of creatine kinase activity, which comprises a carrier, a dehydrogenase, a diaphorase, NAD or NADP, and a water-soluble tetrazolium compound.

公开(公告)号：EP0973938A1

公开(公告)日：2000-01-26

申请号：EP98904810.3

申请日：1998-02-03

申请人： Specialty Assays, Inc.

发明人： KAUFMAN, Richard, A.

IPC分类号： C12Q1/48 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/58 ,  C12Q1/42 ,  C12Q1/54 ,  C12Q1/37 ,  C12Q1/50 ,  C12Q1/32 ,  G01N33/53 ,  C07H19/04

CPC分类号： C07H19/10 ,  Y10S435/975

摘要： Kits and methods for measuring enzyme activities and metabolites using NADH and NADPH analogs are disclosed. The analogs have extended stability in aqueous solutions and can be used as replacements for NADH or NADPH cofactors in analytical procedures. Preferred aspects of the invention include kits containing the NADH and NADPH analogs for use in the measurement of ALT activity, AST activity, Urea, Ammonia, Salicylate, Triglycerides, Pyrubic Acid, Sorbitol Dehydrogenase activity, 5'-Nucleotidase activity, Creatine Kinase activity, 2,3-Diphosphoglyceric Acid, Adenosine 5'-triphosphate, α-Hydroxybutyrate Dehydrogenase activity, Lactate Dehydrogenase activity and the Carbon Dioxide content in analytical samples.

公开(公告)号：EP0458901B1

公开(公告)日：1997-12-17

申请号：EP90904105.5

申请日：1990-02-14

申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  HARVARD UNIVERSITY ,  THE GENERAL HOSPITAL CORPORATION ,  AMIRA, INC.

发明人： KADDURAH-DAOUK, Rima ,  DAOUK, Ghaleb ,  SCHIMMEL, Paul, R. ,  KINGSTON, Robert ,  LILLIE, James, W. ,  GREEN, Michael ,  PUTNEY, Scott, D.

IPC分类号： A61K38/55 ,  C12Q1/50

CPC分类号： C12N15/1137 ,  A61K31/00 ,  A61K31/195 ,  A61K31/415 ,  A61K31/505 ,  A61K31/66 ,  A61K38/00 ,  A61K48/00 ,  C07K14/82 ,  C12N9/12 ,  C12N9/1223 ,  C12Q1/50 ,  C12Y207/0102 ,  C12Y207/03002 ,  C12Y207/04003 ,  C12Y406/01001 ,  G01N33/573 ,  G01N33/57484 ,  G01N2333/01

摘要： A method of inhibiting growth rate, transformation or metastasis of mammalian cells, particularly epithelial cells, in which activity of at least one enzyme which participates in purine metabolism and regulation of nucleotide levels is elevated. In particular, a method of inhibiting transformation of mammalian cells by a DNA tumor virus, a DNA tumor virus factor or other factor which has an equivalent effect on cells.

公开(公告)号：EP0437254B1

公开(公告)日：1995-12-27

申请号：EP91100241.8

申请日：1991-01-09

申请人： BOEHRINGER MANNHEIM GMBH

发明人： Siedel, Joachim, Dr. ,  Vogt, Bernd, Dr.

IPC分类号： C12Q1/34 ,  C12Q1/50

CPC分类号： C12Q1/34 ,  C12Q1/50 ,  G01N2333/978 ,  G01N2333/98

公开(公告)号：EP0351117B1

公开(公告)日：1995-02-22

申请号：EP89306725.6

申请日：1989-07-03

申请人： DADE INTERNATIONAL INC.

发明人： Posner, Alan ,  Romero, Pedro

IPC分类号： C12Q1/32 ,  C12Q1/50

CPC分类号： G01N33/96 ,  C12Q1/007 ,  C12Q1/32 ,  C12Q1/50 ,  G01N2496/05 ,  G01N2496/80 ,  Y10T436/10 ,  Y10T436/105831 ,  Y10T436/108331

公开(公告)号：EP0467782A1

公开(公告)日：1992-01-22

申请号：EP91401996.3

申请日：1991-07-17

申请人： INTERNATIONAL IMMUNOASSAY LABORATORIES, INC.

发明人： Shah, Vipin D. ,  Yen, Shing-Erh ,  Anchin, Gerald M.

IPC分类号： G01N33/573 ,  C12Q1/50

CPC分类号： G01N33/573 ,  C07K16/40

摘要： Methods and reagents for determining the lapse of time since an acute disease event, such as the occurrence of a myocardial infarction, are presented. A serum or plasma sample is assayed to determine the concentration of two analyte sets. From these measurements, the time of the acute event can be more accurately determined. Novel antibodies, labeled and insolubilized derivatives of these antibodies, labeled proteins, and kits containing one or more of these reagents are also described.

摘要翻译： 提出了用于确定急性疾病事件之后的时间流逝的方法和试剂，例如心肌梗死的发生。 测定血清或血浆样品以确定两个分析物组的浓度。 从这些测量可以更准确地确定急性事件的时间。 还描述了这些抗体的新型抗体，标记和不溶性衍生物，标记的蛋白质和含有一种或多种这些试剂的试剂盒。

公开(公告)号：EP0401641A2

公开(公告)日：1990-12-12

申请号：EP90110104.8

申请日：1990-05-28

申请人： ABBOTT LABORATORIES

发明人： Abbott, William A.

IPC分类号： C12Q1/00 ,  C12Q1/32 ,  C12Q1/50 ,  C12Q1/42 ,  C12Q1/52

CPC分类号： C12Q1/00 ,  C12Q1/32

摘要： The present invention discloses a rapid assay method for quantitatively determining the complete isoenzyme profile of a biological fluid and a multiple-assay reagent system for carrying out the method. In one preferred embodiment, a multiple-assay reagent system is disclosed for quantifying the complete isoenzyme profile of lactate dehydrogenase in blood serum, based on two measured parameters and three performed assays.

摘要翻译： 本发明公开了一种用于定量测定生物体液的完全同工酶谱和用于实施该方法的多重测定试剂系统的快速测定方法。 在一个优选实施方案中，公开了用于定量血清中乳酸脱氢酶的完全同工酶谱的多重测定试剂系统，基于两个测量参数和三个进行的测定。

公开(公告)号：EP0227440A3

公开(公告)日：1989-01-18

申请号：EP86309906.5

申请日：1986-12-18

申请人： E.I. DU PONT DE NEMOURS AND COMPANY

发明人： Pankratz, Thomas John

IPC分类号： G01N33/573 ,  G01N33/543 ,  C12Q1/50

CPC分类号： G01N33/542 ,  G01N33/573

摘要： An immunoassay for CK-MB is provided based on the sequential immunoinhibition first by an immobilized and then by a soluble antibody of either the CK-M or the CK-B sub-units in CK-MM, CK-MB and CK-BB, followed by an enzymatic determination of the unbound sub-unit in CK-MB (the unbound CK-BB or CK-MM having been previously removed).