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107 条记录 (耗时 0.033 秒)

    TREATMENT OF HUMAN SOFT TISSUE SARCOMA WITH ADENOVIRAL VECTOR EXPRESSING TNF-ALPHA
    62.
    发明授权
    TREATMENT OF HUMAN SOFT TISSUE SARCOMA WITH ADENOVIRAL VECTOR EXPRESSING TNF-ALPHA 有权
    SARKOM用于与腺病毒载体TNF-ALPHA人民处理

    公开(公告)号：EP1455840B1

    公开(公告)日：2009-01-07

    申请号：EP02776411.7

    申请日：2002-11-01

    申请人： GENVEC, INC.

    发明人： RASMUSSEN, Henrik, S. ,  CHU, Karen, W.

    IPC分类号： A61K48/00 ,  C12N15/861

    CPC分类号： A61K35/761 ,  A61K38/191 ,  C12N2710/10332 ,  C12N2710/10341 ,  A61K2300/00

    摘要： The present invention provides a method for reducing the size of a tumor in a human comprising (a) directly injecting into the tumor, via multiple injections to different points of the tumor, a dose of a pharmaceutical composition comprising (i) a pharmaceutically acceptable carrier and (ii) a replication-deficient adenoviral vector comprising a nucleic acid sequence encoding TNF-alpha operably linked to a promoter, wherein the dose comprises about 1x107 to about 4x1012 3 particle units (pu) of replication-deficient adenoviral vector, two or more times over a therapeutic period comprising up to 10 weeks, and (b) administering a dose of ionizing radiation over the duration of the therapeutic period, whereby the size of the tumor is reduced.

    Vectors and methods for gene transfer to cells
    67.
    发明公开
    Vectors and methods for gene transfer to cells 失效
    Zenen的Vektoren und Methoden zum Gentransfer

    公开(公告)号：EP1616961A1

    公开(公告)日：2006-01-18

    申请号：EP05021223.2

    申请日：1996-11-27

    申请人： GENVEC, INC.

    发明人： Wickham, Thomas J., c/o EMD Lexigen Bedford Campus ,  Brough, Douglas E. ,  Kovesdi, Imre

    IPC分类号： C12N15/861 ,  C07K14/075 ,  C12N7/01 ,  A61K48/00

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C07K14/005 ,  C07K2319/00 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2810/40 ,  C12N2810/405 ,  C12N2810/60

    摘要： The present invention provides a chimeric adenovirus coat protein, which differs from the wild-type coat protein by the introduction of a nonnative amino acid sequence. Such a chimeric adenovirus coat protein according to the invention is able to direct entry into cells of a vector comprising the coat protein that is more efficient than entry into cells of a vector that is identical except for comprising a wild-type adenovirus coat protein rather than the chimeric adenovirus coat protein. The chimeric coat protein preferably is a fiber, hexon, or penton protein. The present invention also provides an adenoviral vector that comprises the chimeric adenovirus coat protein, as well as methods of constructing and using such a vector.

    摘要翻译： 本发明提供了通过引入非氨基酸序列与野生型外壳蛋白不同的嵌合腺病毒外壳蛋白。 根据本发明的这种嵌合腺病毒外壳蛋白能够直接进入包含外壳蛋白的载体的细胞中，所述载体比除了包含野生型腺病毒外壳蛋白的载体以外的载体的细胞进入更有效，而不是 嵌合腺病毒外壳蛋白。 嵌合外壳蛋白质优选为纤维，六邻体或五倍子蛋白。 本发明还提供了包含嵌合腺病毒外壳蛋白的腺病毒载体，以及构建和使用这种载体的方法。

    TREATMENT OF HUMAN CANCER WITH ADENOVIRAL VECTOR EXPRESSING TNF-ALPHA
    69.
    发明公开
    TREATMENT OF HUMAN CANCER WITH ADENOVIRAL VECTOR EXPRESSING TNF-ALPHA 有权
    SARKOM用于与腺病毒载体TNF-ALPHA人民处理

    公开(公告)号：EP1455840A2

    公开(公告)日：2004-09-15

    申请号：EP02776411.7

    申请日：2002-11-01

    申请人： GENVEC, INC.

    发明人： RASMUSSEN, Henrik, S. ,  CHU, Karen, W.

    IPC分类号： A61K48/00 ,  C12N15/861

    CPC分类号： A61K35/761 ,  A61K38/191 ,  C12N2710/10332 ,  C12N2710/10341 ,  A61K2300/00

    摘要： The present invention provides a method for reducing the size of a tumor in a human comprising (a) directly injecting into the tumor, via multiple injections to different points of the tumor, a dose of a pharmaceutical composition comprising (i) a pharmaceutically acceptable carrier and (ii) a replication-deficient adenoviral vector comprising a nucleic acid sequence encoding TNF-alpha operably linked to a promoter, wherein the dose comprises about 1x107 to about 4x1012 3 particle units (pu) of replication-deficient adenoviral vector, two or more times over a therapeutic period comprising up to 10 weeks, and (b) administering a dose of ionizing radiation over the duration of the therapeutic period, whereby the size of the tumor is reduced.

    METHOD OF PRODUCING ADENOVIRAL VECTOR STOCKS
    70.
    发明公开
    METHOD OF PRODUCING ADENOVIRAL VECTOR STOCKS 有权
    工艺生产腺病毒

    公开(公告)号：EP1268748A1

    公开(公告)日：2003-01-02

    申请号：EP00973440.1

    申请日：2000-10-10

    申请人： GENVEC, INC.

    发明人： LIU, Lee-Cheng ,  LAI, Shoupeng

    IPC分类号： C12N7/00 ,  C12N5/00

    CPC分类号： C12N15/86 ,  C12N2710/10343 ,  C12N2710/10351

    摘要： The present invention provides a method of producing an adenoviral vector stock by providing a culture of cells permissive for growth of adenoviral vectors, wherein the cells are in a medium, culturing the culture under conditions to permit growth of the cells, perfusing fresh medium trough the culture for a period of about 1-6 hours, in an amount of at least about two times the volume of medium in the culture, while the density of the cells in the medium is about 40-70 % of the density of cells obtained in the medium when the growth of the culture is in the stationary phase, contacting the culture with adenoviral vectors under conditions permissive for the infection of the cells, culturing the infected cells to replicate the adenoviral vectors, and harvesting the infected cells to obtain an adenoviral vector stock. A method of obtaining a synchronous culture of cells is also provided.