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    Process for controlling contamination of nucleic acid amplification reactions
    63.
    发明公开
    Process for controlling contamination of nucleic acid amplification reactions 失效
    Verfahren zur Beherrschung von Kontamination derNukleinsäure-Amplifikation。

    公开(公告)号：EP0401037A2

    公开(公告)日：1990-12-05

    申请号：EP90306001.0

    申请日：1990-06-01

    申请人： LIFE TECHNOLOGIES INC.

    发明人： Hartley, James L.

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/708 ,  C12Q1/6848 ,  C12Q2531/113 ,  C12Q2525/101

    摘要： An amplification procedure is performed on a first sample in which one or more of the four normal ribonucleoside triphosphates(rNTPs) or deoxyribonucleoside triphosphates (dNTPs) is replaced with an exo-sample nucleotide that is not present in nucleic acid to be amplified. After amplification, any contaminating amplified product that may be remaining is subjected to a physical, chemical, enzymatic, or biological treatment which renders nucleic acid containing the exo-sample nucleotide substantially unamplifiable. The treatment may be done as a separate step or it may be done in the presence of a second sample containing nucleic acid sequences to be amplified. The amplified nucleic acid sequences derived from the first sample which contaminate the second sample are not further substantially amplified during amplification of nucleic acid sequences of the second sample.

    摘要翻译： 对其中四种正常核糖核苷三磷酸（rNTP）或脱氧核糖核苷三磷酸（dNTP）中的一种或多种被不存在于待扩增的核酸中的外切核苷酸替代的第一样品进行扩增程序。 扩增后，可能残留的任何污染的扩增产物进行物理，化学，酶学或生物处理，使含有外切核苷酸的核酸基本上不可扩增。 治疗可以作为单独的步骤进行，或者可以在含有待扩增的核酸序列的第二个样品的存在下进行。 衍生自污染第二样品的第一样品的扩增的核酸序列在第二样品的核酸序列的扩增期间不被进一步基本上扩增。

    A composition for use in the treatment of fish material during the preparation of a processed fish product, to a method of preparing such a fish product and to an improved fish product
    64.
    发明公开
    A composition for use in the treatment of fish material during the preparation of a processed fish product, to a method of preparing such a fish product and to an improved fish product 失效
    一种用于鱼产品，这样的鱼产品的制备方法的制备过程中在鱼材料的处理组合物的使用和改进的鱼制品。

    公开(公告)号：EP0401004A2

    公开(公告)日：1990-12-05

    申请号：EP90305897.2

    申请日：1990-05-31

    申请人： LIFE TECHNOLOGIES INC.

    发明人： Lorier, Michel Albert ,  Aitken, Brian Lawrence

    IPC分类号： A23L1/325

    CPC分类号： A23B4/20 ,  A23L17/70 ,  C07K14/8107

    摘要： A large number of fish species exhibit protease activity and are therefore unacceptable for preparing processed fish products such as surimi. The present invention is directed to a composition containing alpha-2-macroglobulin for inhibiting protease activity contained in the fish material during the preparation of a processed fish product. Thus, the invention provides suitable proteases inhibitors to treat a number of fish species which were previously unsuitable for preparing processed fish products such as surimi.
    The present invention also relates to a method of preparing a processed fish product comprising contacting the fish material with alpha-2-macroglobulin in an amount sufficient to neutralize or inhibit the protease contained in the fish material.
    In addition, the present invention is directed to a fish product prepared using the composition of this invention and to a fish product prepared according to the method of this invention.

    摘要翻译： 大量的鱼类的表现出蛋白酶活性，并因此用于制备加工的鱼产品不可接受的：如鱼糜。 本发明涉及含有α-2巨球蛋白一个加工鱼产品的制备过程中用于抑制包含在鱼材料蛋白酶活性的组合物。 因此，本发明提供了一种适合的蛋白酶抑制剂治疗许多种鱼类这在以前是不适合用于制备加工的鱼制品：如鱼糜。 因此，本发明涉及一种制备经加工的鱼产品，其包括使与α-2巨球蛋白的鱼材料量足以中和或抑制包含在鱼材料蛋白酶的方法。 另外，使用本发明的一个鱼产品的组合物制备的鱼产物制备雅丁本发明的方法中，本发明涉及。