公开(公告)号：EP2198018A2

公开(公告)日：2010-06-23

申请号：EP08830789.7

申请日：2008-09-13

申请人： Codexis, Inc.

发明人： LIANG, Jack ,  JENNE, Stephane, J. ,  MUNDORFF, Emily ,  CHING, Charlene ,  GRUBER, John, M. ,  KREBBER, Anke ,  HUISMAN, Gjalt, W.

IPC分类号： C12N9/04

CPC分类号： C12N9/0006 ,  C12P7/22 ,  C12Y101/01 ,  C12Y101/01184 ,  Y02E50/17 ,  Y02P20/52

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

摘要翻译： 本公开提供了与天然存在的野生型酮还原酶相比具有改进性质的工程化酮还原酶。 还提供了编码工程化酮还原酶的多核苷酸，能够表达工程化酮还原酶的宿主细胞，以及使用工程化酮还原酶合成各种手性化合物的方法。

公开(公告)号：EP1940456A2

公开(公告)日：2008-07-09

申请号：EP06825457.2

申请日：2006-10-04

申请人： THE OHIO STATE UNIVERSITY RESEARCH FOUNDATION

发明人： CROCE, Carlo, M. ,  FABBRI, Muller ,  TRAPASSO, Francesco

IPC分类号： A61K38/44 ,  A61K38/43 ,  C12N15/00

CPC分类号： A61K38/443 ,  A61K48/005 ,  A61K48/0075 ,  C12N15/86 ,  C12N2710/10343 ,  C12Y101/01

摘要： The present invention provides novel methods and compositions for the diagnosis, prognosis and treatment of cancer in a subject, by administering to the subject a polynucleotide encoding a functional WWOX gene product.

公开(公告)号：EP1761628A2

公开(公告)日：2007-03-14

申请号：EP05752264.1

申请日：2005-06-17

申请人： HENKEL KOMMANDITGESELLSCHAFT AUF AKTIEN

发明人： BESSLER, Cornelius ,  FEESCHE, Jörg ,  EVERS, Stefan ,  MAURER, Karl-Heinz ,  EHRENREICH, Armin ,  VEITH, Birgit ,  LIESEGANG, Heiko ,  HENNE, Anke ,  HERZBERG, Christina ,  GOTTSCHALK, Gerhard

IPC分类号： C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/12 ,  C12N9/78 ,  C12N9/88

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1217 ,  C12N9/16 ,  C12N9/78 ,  C12N9/80 ,  C12N9/84 ,  C12N9/88 ,  C12N9/93 ,  C12N15/75 ,  C12P7/16 ,  C12P7/40 ,  C12P7/42 ,  C12P7/52 ,  C12P13/001 ,  C12Y101/01 ,  C12Y101/01035 ,  C12Y101/01157 ,  C12Y102/04002 ,  C12Y103/99 ,  C12Y203/01019 ,  C12Y206/01042 ,  C12Y207/02007 ,  C12Y305/01001 ,  C12Y305/01011 ,  C12Y401/01018 ,  C12Y401/01019 ,  C12Y402/01017 ,  C12Y402/01055 ,  C12Y602/01 ,  C12Y602/01001 ,  Y02E50/10 ,  Y02P20/52

摘要： The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived therefrom and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and/or isobutyric acid (as part of the catabolism of valine and/or isoleucine), 3) butanol and/or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and/or 5) cadaverine and/or putrescine (as parts of the catabolism of lysine and/or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesised via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their respective biochemical properties.

摘要翻译： 二十五中细菌的生物合成所涉及的核酸序列（A），特别是在异戊酸，2-甲基，和异丁propylic酸，丁醇，尸胺和/或腐胺的芽孢杆菌licheniformisDSM 13是新的。 因此独立权利要求中包括了：（1）的基因产物（B）（A）; （2）在其中的微生物发酵的至少一个基因，参与代谢途径（ⅰ）异戊酸，（ⅱ）2-甲基和/或异丁酸，（III）丁醇和/或丁酸，（ⅳ ）propylic酸或（v）尸胺和/或腐胺，已经失活; （3）使用用于（i）的合成代谢途径的功能性失活的特定基因的 - 上述（v）的; （4），其中至少一个（A）的微生物已经失活; 和（5）的微生物发酵（4）。