公开(公告)号：EP0148333A1

公开(公告)日：1985-07-17

申请号：EP84112251.8

申请日：1984-10-11

申请人： CETUS CORPORATION

发明人： Williams, Fred Gardner

IPC分类号： B01L3/02

CPC分类号： B01L3/0279

摘要： A disposable pipette tip (10) is formed for automatic installation and removal with low friction between the tip and pipette body (16) and a predetermined distance from the pipette body end (19) and the tip intake/discharge opening (12). The tip has a conical section (11) whose small end defines the tip intake/discharge opening and a cylindrical collar (14) that receives the pipette body. The tip is formed with an internal pipette seat (17) at the junction of the conical section and cylindrical collar so that the distance between the tip inlet/discharge opening and the pipette body end is fixed. The cylindrical collar also has a circumferential sealing rib (30) axially spaced from the pipette seat to form an air seal to account for manufacturing tolerances in the tip's cylindrical portion as to both diameter and roundness while maintaining low friction for installation and removal.

摘要翻译： 形成一次性吸头（10），用于自动安装和移除尖端和移液管本体（16）之间的低摩擦力并与吸液管本体端部（19）和尖端进入/排出口（12）预定距离。 尖端具有锥形部分（11），其小端限定尖端进入/排出口和容纳移液管主体的圆柱形套环（14）。 尖端在锥形部分和圆柱形套环的接合部处形成有内部移液管座（17），使得尖端入口/排出口与移液管主体端部之间的距离是固定的。 圆柱形套环还具有与移液器座轴向间隔开的周向密封肋（30），以形成空气密封，以便在直径和圆度两者之间考虑尖端圆柱形部分中的制造公差，同时降低用于安装和拆卸的低摩擦力。

公开(公告)号：EP0135159A2

公开(公告)日：1985-03-27

申请号：EP84109813.0

申请日：1984-08-17

申请人： CETUS CORPORATION

发明人： Huang, Chun-Ming ,  Cohen, Stanley Norman

IPC分类号： C12P21/00 ,  C12N5/00 ,  G01N33/577 ,  C12Q1/68 ,  C12N15/00 ,  C07H21/04 ,  C12P19/34

CPC分类号： G01N33/5308 ,  C07K16/18 ,  C12Q1/6804 ,  Y10S435/948 ,  Y10S435/962 ,  Y10S530/809 ,  Y10S530/864

摘要： Described are monoclonal antibodies having conformation-dependent specificity for native dsDNA, as exemplified by the IgM antibody produced by murine hybridoma ATCC No. HB 8329, have been prepared. These antibodies may be bound to double-stranded DNA to form immune complexes and thus are useful in immunodiagnostic tests to detect DNA duplex formation by DNA hybridization and to separate dsDNA from a solution containing it.

摘要翻译： 描述的是具有用于天然双链DNA构象依赖性特异性单克隆抗体，举例说明由鼠杂交瘤ATCC号产生的IgM的抗体 HB 8329，已准备。 这些抗体可以结合到双链DNA，以形成免疫复合物并因此在免疫诊断测试中有用的，以检测通过DNA杂交的DNA双链体形成和与dsDNA从含有它的溶液中分离出来。

公开(公告)号：EP0109748A1

公开(公告)日：1984-05-30

申请号：EP83306221.9

申请日：1983-10-13

申请人： CETUS CORPORATION

发明人： Mark, David F. ,  Lin, Leo S. ,  Yu Lu, Shi-Da

IPC分类号： C12N15/00 ,  C12P21/02 ,  A61K37/02

CPC分类号： C07K14/55 ,  A61K38/00 ,  C07K14/565 ,  C12N15/102 ,  Y10S530/809 ,  Y10S930/141 ,  Y10S930/142

摘要： The invention provides muteins of biologically active proteins such as IFN-B and IL-2 in which cysteine residues that are not essential to biological activity have been deleted or replaced with other amino acids to eliminate sites for intermolecular crosslinking or incorrect intramolecular disulfide bridge formation. These muteins may be made via bacterial expression of mutant genes that encode the muteins and have been synthesized from the genes for the parent proteins by oligonucleotide-directed mutagenesis.

摘要翻译： 本发明提供了生物活性蛋白的突变蛋白：例如IFN-β和IL-2，其中半胱氨酸残基确实不是已被删除或用其它氨基酸取代，以消除分子间交联的或不正确的分子内二硫键形成位点至关重要的生物活性。 这些突变蛋白可通过突变体的基因的细菌表达制成并编码突变蛋白，并从基因通过寡核苷酸定向诱变的亲本蛋白质havebeen合成。

公开(公告)号：EP0103408A2

公开(公告)日：1984-03-21

申请号：EP83304661.8

申请日：1983-08-11

申请人： CETUS CORPORATION

发明人： Neidleman, Saul Lewis ,  Ervin, Jacqueline Lutricia

IPC分类号： C12P7/64 ,  C10M105/34 ,  C10M129/70 ,  C12R1/01

CPC分类号： C12P7/6463 ,  C10M105/34 ,  C10M129/70 ,  C10M2207/281 ,  C10M2207/282 ,  C10M2207/283 ,  C10M2207/286 ,  C10N2240/402 ,  C10N2240/403 ,  C10N2240/404 ,  C10N2240/405 ,  C10N2240/406 ,  C10N2240/407 ,  C10N2240/408 ,  C10N2240/409 ,  Y10S435/822 ,  Y10S435/859

摘要： A process for producing wax esters having the general structural formula RCO 2 R', where R and R' are radicals selected from the group consisting of CH 3 (CH 2 ) a CH=CH(CH 2 ) b - and CH 3 (CH 2 ) c - where a + b = 12-14 for R and 13-15 for R' and where c = 14-16 for R and 15-17 for R'. The method includes aerobically incubating a culture of microorganisms of the genus Acinetobacter species HOI-N, in an aqueous mineral salts solution containing ethanol as a primary food source. Incubation is performed at a temperature known to produce a desired percentage of diene, monoene and saturated R and R' moieties in the mixture.

公开(公告)号：EP0098864A1

公开(公告)日：1984-01-25

申请号：EP83900460.0

申请日：1983-01-11

申请人： CETUS CORPORATION

发明人： INNIS, Michael A.

IPC分类号： C07K14 ,  A61K38

CPC分类号： C07K14/56 ,  A61K38/00 ,  Y02A50/473

摘要： New polypeptide, called IFN- alpha 74, produced by E.coli transformed with a newly isolated and characterized human IFN- alpha gene. The polypeptide exhibits interferon activities such as antiviral activity, cell growth regulation, and regulation of production of cell-produced substances.

公开(公告)号：EP0098863A1

公开(公告)日：1984-01-25

申请号：EP83900459.0

申请日：1983-01-11

申请人： CETUS CORPORATION

发明人： INNIS, Michael A.

IPC分类号： C07K14 ,  A61K38

CPC分类号： C07K14/56 ,  A61K38/00 ,  Y02A50/473 ,  Y10S435/811

摘要： A new polypeptide, called IFN- alpha 61, produced by E. coli transformed with a newly isolated and characterized human IFN- alpha gene is described. The polypeptide exhibits interferon activities such as antiviral activity, cell growth regulation, and regulation of production of cell-produced substances.

公开(公告)号：EP0096586A2

公开(公告)日：1983-12-21

申请号：EP83303293.1

申请日：1983-06-07

申请人： CETUS CORPORATION

发明人： Gelfand, David Harow ,  Strange, Carolyn Jan ,  Watson, Robert Malcolm

IPC分类号： C12N15/63 ,  C12N1/20 ,  C12P21/00

CPC分类号： C07K14/565 ,  C12N15/69 ,  C12N15/70

摘要： The invention provides plasmids of ColEl type parentage which have a temperature sensitive replication system such that the plasmid copy number in a given host cell may be controlled by a temperature shift. Such plasmids permit the use of temperature as a switch for controlling gene amplification and hence the production of corresponding protein in a host cell.

摘要翻译： 本发明提供了Co1E1型亲子其中具有检测温度敏感型复制系统做了在给定的宿主细胞中的质粒拷贝数可通过温度变化来控制的质粒。 搜索质粒允许使用温度的作为用于控制基因扩增和因此生产的宿主细胞中相应蛋白质的开关。

公开(公告)号：EP0060045A3

公开(公告)日：1983-08-24

申请号：EP82300865

申请日：1982-02-22

申请人： CETUS CORPORATION

发明人： GELFAND, DAVID HARROW

IPC分类号： C12N15/09 ,  C07H21/04 ,  C07K14/005 ,  C07K14/195 ,  C12N1/00 ,  C12N15/00 ,  C12N15/69 ,  C12N15/70 ,  C12P21/00 ,  C12P21/02 ,  C12P21/04

CPC分类号： C12N15/70 ,  C12N15/69

摘要： The problem of producing stable DNA plasmids of high copy number may be solved by selecting mutants in which an altered repressor gene leads to high copy number replication. The plasmids are modifed by placing a suitable transcriptional "stop" signal after an inserted heterogenous gene so that the readthrough expression of the heterologous DNA will not continue into the replication primer strand. Deletions resulting from interference with replication primer strand transcription are thereby avoided.
Such plasmids may be used to produce a desired protein by introducing them into a host microorganism and allowing the host to express phenotypically its genotype (including the plasmids).

摘要翻译： 通过选择其中改变的阻遏物基因导致高拷贝数复制的突变体，可以解决产生高拷贝数的稳定DNA质粒的问题。 通过在插入的异源基因之后放置合适的转录“停止”信号来修饰质粒，使得异源DNA的读取表达不会继续进入复制引物链。 从而避免了由于干扰复制引物链转录而导致的缺失。 这样的质粒可用于通过将其引入宿主微生物并使宿主表达其基因型（包括质粒）来产生所需的蛋白质。

公开(公告)号：EP0084796A3

公开(公告)日：1983-08-10

申请号：EP83100175

申请日：1983-01-11

申请人： CETUS CORPORATION

发明人： Erlich, Henry A.

IPC分类号： C12N15/00 ,  C12Q01/68

CPC分类号： C12Q1/6881 ,  C07K14/70539 ,  C12Q1/683 ,  C12Q2600/156 ,  C12Q2600/172

摘要： HLA typing based on restriction length polymorphism is carried out by: digesting an individual's HLA DNA with a restriction endonuclease that produces a polymorphic digestion pattern with HLA DNA; subjecting the digest to genomic blotting using a labeled cDNA hybridization probe that is complementary to an HLA DNA sequence involved in the polymorphism; and comparing the resulting genomic blotting pattern with a standard This technique may be adapted to make paternity or transplant or transfusion compatibility determinations or to make disease association correlations to diagnose diseases or predict susceptibility to diseases. Locus specific cDNA hybridization probes, particularly probes for genes of Class II loci (D and OR loci), for use in the typing procedure are described.

公开(公告)号：EP0084796A2

公开(公告)日：1983-08-03

申请号：EP83100175.5

申请日：1983-01-11

申请人： CETUS CORPORATION

发明人： Erlich, Henry A.

IPC分类号： C12N15/00 ,  C12Q1/68

CPC分类号： C12Q1/6881 ,  C07K14/70539 ,  C12Q1/683 ,  C12Q2600/156 ,  C12Q2600/172

摘要： HLA typing based on restriction length polymorphism is carried out by: digesting an individual's HLA DNA with a restriction endonuclease that produces a polymorphic digestion pattern with HLA DNA; subjecting the digest to genomic blotting using a labeled cDNA hybridization probe that is complementary to an HLA DNA sequence involved in the polymorphism; and comparing the resulting genomic blotting pattern with a standard This technique may be adapted to make paternity or transplant or transfusion compatibility determinations or to make disease association correlations to diagnose diseases or predict susceptibility to diseases. Locus specific cDNA hybridization probes, particularly probes for genes of Class II loci (D and OR loci), for use in the typing procedure are described.

摘要翻译： 基于限制性长度多态性的HLA分型是通过用限制性内切核酸酶消化个体的HLA DNA，其产生具有HLA DNA的多态性消化模式; 使用与涉及多态性的HLA DNA序列互补的标记的cDNA杂交探针对消化片进行基因组印迹; 并将得到的基因组印迹图案与标准物进行比较。 这种技术可以适应于做出亲子鉴定或移植或输血相容性测定，或者使疾病关联相关性诊断疾病或预测对疾病的易感性。 描述了用于敲入程序的特异性cDNA杂交探针，特别是用于II类基因座（D和DR基因座）的基因的探针。