公开(公告)号：EP2535151A1

公开(公告)日：2012-12-19

申请号：EP11004979.8

申请日：2011-06-17

申请人： EPPENDORF AG

发明人： Schirr, Andreas ,  Graff, Andreas

IPC分类号： B25J7/00 ,  C12N15/89

CPC分类号： G05B15/02 ,  C12M99/00

摘要： The invention relates to an actuator apparatus (1) for generating the motion of a tool, in particular for the work on biological cell material, which provides at least one electrically controlled actuator element (3), a motion section (3a), at which a tool can be arranged and which is linked to the at least one actuator element, an electrical control device (11) for controlling the at least one actuator element, an electrical measurement device (12), which is configured to perform a measuring method for measuring at least one electrical capacitance quantity of the at least one actuator element, wherein the capacitance quantity is usable to provide information on the status of the actuator apparatus. Further, a corresponding method for obtaining and utilizing said information on the actuator apparatus is provided.

摘要翻译： 本发明涉及一种致动器装置（1），用于产生工具的运动，特别是用于生物细胞材料上的工作，其提供至少一个电控致动器元件（3），运动部分（3a） 可以布置工具并且连接到至少一个致动器元件，用于控制至少一个致动器元件的电气控制装置（11），电测量装置（12），其被配置为执行用于 测量所述至少一个致动器元件的至少一个电容量，其中所述电容量可用于提供关于所述致动器装置的状态的信息。 此外，提供了用于在致动器装置上获得和利用所述信息的相应方法。

公开(公告)号：EP2522734A2

公开(公告)日：2012-11-14

申请号：EP12167500.3

申请日：2012-05-10

申请人： IUL, S.A.

发明人： Font Castell, Victor

IPC分类号： C12N15/89 ,  C12M3/00

CPC分类号： C12M35/00

摘要： The invention relates to those apparatus that include a disposable microsyringe, whose tubular body is attached by its rear end into the front starting point of a longitudinal void of an intermediate housing, locating in said longitudinal void a moving head movable upon an axis and, which is connected to the rear end of the syringe plunger. It is characterised in that the moving head (9) is also connected, by means of a longitudinal rod (12), to a moving carriage (13) movable upon an axis along the same direction as the moving head (9), and being said moving carriage (13) guided within an elongated tube (8) connected to at the rear end of the intermediate housing (7), as a continuation thereof, incorporating to said elongated tube (8) a motor element, whose actuation drives the plunger (10) by means of a moving head (9), a longitudinal rod (12) and a moving carriage (13).

摘要翻译： 本发明涉及包括一次性微量注射器的那些装置，其管状体通过其后端连接到中间壳体的纵向空隙的前起始点，位于所述纵向空隙中，移动头可在轴线上移动，并且其中 连接到注射器柱塞的后端。 其特征在于，移动头（9）还通过纵向杆（12）连接到沿着与移动头（9）相同方向的轴线上可移动的移动滑架（13），并且 所述移动滑架（13）在连接到中间壳体（7）的后端的细长管（8）中引导，作为其延续，并入所述细长管（8）中的马达元件，其致动驱动柱塞 （10）通过移动头（9），纵向杆（12）和移动滑架（13）。

公开(公告)号：EP2452192A1

公开(公告)日：2012-05-16

申请号：EP10734358.4

申请日：2010-07-08

申请人： ZF SCREENS B.V. ,  Universiteit Leiden

发明人： SPAINK, Herman, Pieter ,  DIRKS, Ronald, Petronella, Hubertus

IPC分类号： G01N33/50 ,  C12N15/89 ,  C12M3/00

CPC分类号： G01N33/5082 ,  G01N33/5011 ,  G01N33/5023 ,  G01N33/5088

摘要： Provided is a method and system for screening chemical compounds or compositions, wherein replicating entities are introduced into the yolk of an (un)fertilized egg or embryo. The method may be extended to elucidate the mechanism-of-action of functional chemical compounds or compositions in the same method and system. The method and system may also be employed for identifying marker genes, marker proteins or marker metabolites.

公开(公告)号：EP2135951B1

公开(公告)日：2012-05-16

申请号：EP08715174.2

申请日：2008-03-07

申请人： Staidson (Beijing) BioPharmaceutical Co., Ltd

发明人： ZHOU, Zhiwen ,  CHEN, Hongbo ,  ZHANG, Hongshan

IPC分类号： C12N15/12 ,  A01K67/027 ,  C07K14/48 ,  C12N15/63 ,  C12N15/85 ,  C12N15/89 ,  C12N15/90 ,  A61K38/18 ,  A61P25/00

CPC分类号： C07K14/475 ,  A01K67/0275 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/072 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  A61K38/00 ,  C07K14/48 ,  C12N15/8509

摘要： Transgenic rodents having NGF beta gene mutants in their genomes express NGF beta mutant proteins. The preparation methods of the transgenic rodents, the methods of utilizing the transgenic animals to prepare NGF beta mutant proteins and the resulting NGF beta mutant proteins are provided. The transgenic rodents are useful in preparing human NGF and in the study of the functions of NGF beta mutants and their receptors in the whole animal level, and also useful for screening and purifying NGF beta mutants which have high activity and high security.

公开(公告)号：EP1105469B1

公开(公告)日：2010-09-29

申请号：EP99941019.4

申请日：1999-08-10

申请人： UNIVERSITY OF HAWAII

发明人： YANAGIMACHI, Ryuzo

IPC分类号： C12N15/00 ,  A01K67/027 ,  A01K67/033 ,  C12N15/87 ,  C12N15/89

CPC分类号： A01K67/0275 ,  A01K67/0335 ,  A01K67/0338 ,  A01K2217/05 ,  C12N15/87 ,  C12N15/89

摘要： Coinjection of unfertilized mouse oocytes with sperm heads and exogenous nucleic acid encoding a transgene results in transgene-expressing embryos, reflecting nucleic acid-sperm head association before coinjection. Nonselective transfer to surrogate mothers of embryos resulting from coinjection produced offspring expressing the integrated transgene.

公开(公告)号：EP1752542B1

公开(公告)日：2009-05-27

申请号：EP05735152.0

申请日：2005-04-04

申请人： Consejo Superior de Investigaciones Cientificas ,  Instituto Nacional De Investigacion y Tecnologia agraria y Alimentaria (INIA)

发明人： VENTURA OLIVEIRA MOREIRA, Pedro Nuno ,  GUTIERREZ ADÁN, Alfonso ,  MOTOLIU JOSE , Lluís, CENTRO NAC. DE BIOTECNOLOGIA

IPC分类号： C12N15/89 ,  A01K67/027

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/03 ,  C12N15/8775 ,  C12N2800/206

公开(公告)号：EP1063287A4

公开(公告)日：2006-12-06

申请号：EP99939150

申请日：1999-03-12

申请人： TOUDAI TLO LTD

发明人： KARUBE ISAO ,  SAITOH TAKASHI

IPC分类号： C12M3/00 ,  C12N13/00 ,  C12N15/89 ,  C12M1/00 ,  C12N5/06

CPC分类号： C12N15/89 ,  C12M35/00 ,  C12N13/00

摘要： Techniques for regulating membrane denaturation reactions without resort to any physical shear force. Namely, a method which comprises reacting a stimulus (light, etc.) with a compound which is activated by this stimulus on a membrane such as a biomembrane to thereby induce membrane destruction at a specific site; and membrane structures (cells, etc.) with membrane destruction at a specific site obtained thereby. Use of these membrane structures makes it possible to transfer genes, etc. Materials for causing membrane destruction at a specific site. Use of these techniques makes it possible to easily pierce cell membranes with ultrafine members constituting, for example, microinjectors, micromanipulators or microelectrodes which are hardly usable in piercing cell membranes by the conventional techniques.

公开(公告)号：EP1693444A1

公开(公告)日：2006-08-23

申请号：EP06250278.6

申请日：2006-01-19

申请人： FUJITSU LIMITED

发明人： Youoku, Sachihiro, Fujitsu Limited ,  Ando, Moritoshi, Fujitsu Limited ,  Ito, Akio, Fujitsu Limited

IPC分类号： C12M3/00 ,  C12N15/89

CPC分类号： A61K48/0075 ,  C12M35/00

摘要： A particle fixing unit fixes a particle (19). A needle (12) is arranged in such a manner that an angle between a surface on a side of the particle fixing unit on which the particle is fixed and a longitudinal center axis of the needle becomes equal to or more than 45 degrees and equal to or less than 135 degrees. An image forming unit forms, when observing at least one of the particle and the needle across the particle fixing unit, at least one of an image of the particle and an image of the needle (12), based on a light transmitted through the particle fixing unit.

摘要翻译： 颗粒固定单元固定颗粒（19）。 针（12）的布置方式是使得固定有颗粒的颗粒固定单元的一侧的表面与针的纵向中心轴之间的角度等于或大于45度并且等于 或小于135度。 当通过颗粒定影单元观察颗粒和针中的至少一个时，图像形成单元基于透过颗粒的光而形成颗粒的图像和针（12）的图像中的至少一个 固定单元。

公开(公告)号：EP1211307A4

公开(公告)日：2006-05-10

申请号：EP00956956

申请日：2000-09-06

申请人： TOUDAI TLO LTD

发明人： SAITO TAKASHI

IPC分类号： C12M3/00 ,  C12N15/87 ,  C12N15/89 ,  C12M1/00 ,  C12N15/00

CPC分类号： C12N15/89 ,  C12M35/00 ,  C12N15/87

摘要： A method which comprises bringing a membrane denaturing material, which undergoes a membrane denaturation reaction induced by a stimulus, into contact with at least a part of a membrane (10) or close thereto; thereby stimulating the membrane denaturing material so as to denature the membrane (10); and piercing the membrane (10) by using a membrane breaking member (1); wherein the stimulus is imparted via the membrane breaking member (10). Thus, the effect of the membrane breaking agent on the substance, in which the stimulus is transmembranely injected, can be prevented as far as possible. It is also possible to favorably stimulate a specific site, though the apparatus has a simple constitution.

公开(公告)号：EP1546348A1

公开(公告)日：2005-06-29

申请号：EP03751872.7

申请日：2003-08-20

申请人： Cellectricon AB

发明人： ORWAR, Owe ,  CHIU, Daniel ,  PIHL, Johan ,  SINCLAIR, Jon ,  OLOFSSON, Jessica ,  KARLSSON, Mattias ,  JARDEMARK, Kent ,  FARRE, Cecilia

IPC分类号： C12N15/89 ,  C12M3/00

CPC分类号： G01N33/54373 ,  C12M35/02 ,  G01N33/48728

摘要： The invention provides a system, system components, and a method for rapidly obtaining and stably maintaining a cell in optimal contact with the cell-contacting surface of a sensor in a cell-based biosensor. In one aspect, the system maximizes the seal between a whole cell and the cell-contact surface of a patch clamp micropipette, maximizing the efficiency of a whole cell patch clamp recording.

摘要翻译： 本发明提供了用于在基于细胞的生物传感器中快速获得并稳定地维持细胞与传感器的细胞接触表面最佳接触的系统，系统组件和方法。 一方面，该系统使膜片钳微量移液器的全细胞与细胞接触表面之间的密封最大化，从而使全细胞膜片钳记录的效率最大化。