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    T1r2-t1r3 hetero-oligomeric sweet taste receptors and cell lines that express said receptors and use thereof for identification of sweet taste compounds
    86.
    发明授权
    T1r2-t1r3 hetero-oligomeric sweet taste receptors and cell lines that express said receptors and use thereof for identification of sweet taste compounds 有权
    Heterooligomeric T1R2-T1R3甜味受体和这些表达细胞系，并使用它们的甜味风味化合物的鉴定

    公开(公告)号：EP1412750B1

    公开(公告)日：2012-05-23

    申请号：EP02761016.1

    申请日：2002-06-26

    申请人： Senomyx, Inc.

    发明人： ZOLLER, Mark, T. ,  LI, Xiaodong ,  STASZEWSKI, Lena ,  O'CONNELL, Shawn ,  ZOZULYA, Sergey ,  ADLER, Jon, Elliott ,  XU, Hong ,  ECHEVERRI, Fernando

    IPC分类号： C07K14/705

    CPC分类号： C07K14/705 ,  A01K2217/00 ,  A01K2217/05 ,  G01N2333/726

    摘要： The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners. Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli. Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging. Finally, the invention relates to the discovery that some compounds, e.g., lactisole, inhibit both the activities of human T1R2/T1R3 and T1R1/T1R3 receptors, and accordingly the sweet and umami taste, suggesting that these receptors may be the only sweet and umami receptors.