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    PROCESS FOR PRODUCING APELIN
    83.
    发明公开
    PROCESS FOR PRODUCING APELIN 审中-公开
    VERFAHREN ZUR HERSTELLUNG VON APELIN

    公开(公告)号：EP1138772A1

    公开(公告)日：2001-10-04

    申请号：EP99961284.9

    申请日：1999-12-09

    申请人： Takeda Chemical Industries, Ltd.

    发明人： SUENAGA, Masato ,  ITO, Takashi ,  NISHIMURA, Osamu

    IPC分类号： C12N15/62 ,  C12N15/12 ,  C12N15/18 ,  C12P21/02 ,  C12N1/21 ,  C07K19/00 ,  C07K14/47 ,  C07K14/50 ,  C07K1/107 ,  C07K1/12

    CPC分类号： C07K14/47 ,  C07K2319/00

    摘要： The present invention provides a production method advantageous in producing Apelin on an industrial scale in large amounts.
    The present invention relates to a method for producing Apelin or a salt thereof, comprising subjecting a fused protein or peptide consisting of a protein or peptide having N-terminal cysteine with Apelin optionally having an optionally oxidized methionine residue at the N-terminus thereof joined to the N-terminus of the protein or peptide to a cleavage reaction for the peptide bond on the amino group side of the cysteine residue.
    Using the production method of the present invention, it is possible to produce on an industrial scale in large amounts Apelin that can be used as a therapeutic/preventive agent for such diseases as various forms of dementia, including senile dementia and cerebrovascular dementia, depression, hyperkinetic (minimal brain damage) syndrome, disturbance of consciousness, anxiety disorder, schizophrenia, phobia, impairments of growth hormone secretion, hyperphagia, polyphagia, hypercholesterolemia, hyperglyceridemia, hyperlipidemia, hyperprolactinemia, hypoglycemia, hypopituitarism, pituitary dwarfism, diabetes mellitus, cancers, pancreatitis, renal diseases, Turner's syndrome, neurosis, rheumatoid arthritis, spinal injury, transient cerebral ischemic attack, amyotrophic lateral sclerosis, acute myocardial infarction, spinocerebellar deformation, bone fractures, wounds, atopic dermatitis, osteoporosis, asthma, epilepsy, infertility, arteriosclerosis, pulmonary emphysema, pulmonary edema, and milk secretion insufficiency, and can also be used as a hypnotic sedative, a postoperative nutritional status improving agent, a hypertensive, a hypotensive, a preventive or therapeutic drug for HIV infection, AIDS, etc., and the like.

    摘要翻译： 本发明提供了在工业规模上大量生产Apelin的生产方法。 本发明涉及一种生产Apelin或其盐的方法，其包括将具有N-末端半胱氨酸的蛋白质或肽组成的融合蛋白或肽与任选具有任选氧化的甲硫氨酸残基的Apelin在其N末端连接至 蛋白质或肽的N-末端为半胱氨酸残基的氨基侧上的肽键的裂解反应。 使用本发明的制造方法，可以以工业规模生产大量的可以用作各种形式的老年痴呆症，诸如老年性痴呆，脑血管性痴呆，抑郁症等疾病的治疗预防剂的Apelin， 超动力学（最小脑损伤）综合征，意识障碍，焦虑障碍，精神分裂症，恐惧症，生长激素分泌的损伤，食欲不振，多食，高胆固醇血症，高甘油血症，高脂血症，高催乳素血症，低血糖症，垂体功能减退症，垂体侏儒症，糖尿病， ，肾脏疾病，特纳综合征，神经症，类风湿关节炎，脊髓损伤，短暂性脑缺血发作，肌萎缩性侧索硬化，急性心肌梗死，脊髓小脑变形，骨折，创伤，特应性皮炎，骨质疏松症，哮喘，癫痫，不孕，动脉硬化，肺 肺气肿，肺水肿， d乳分泌不足，也可用作催眠镇静剂，术后营养状态改善剂，高血压药，降血糖药，艾滋病毒感染预防或治疗药物，艾滋病等。

    Dna encoding a growth factor specific for epithelial cells
    89.
    发明公开
    Dna encoding a growth factor specific for epithelial cells 失效
    德国科学院院士Wechstumsfaktor

    公开(公告)号：EP1016716A2

    公开(公告)日：2000-07-05

    申请号：EP00103140.0

    申请日：1990-01-30

    申请人： RUBIN, Jeffrey S ,  FINCH, Paul W ,  AARONSON, Stuart A

    发明人： RUBIN, Jeffrey S ,  FINCH, Paul W ,  AARONSON, Stuart A

    IPC分类号： C12N15/12 ,  C07K14/50 ,  A61K38/18 ,  C12N15/62 ,  C07K16/22

    CPC分类号： C12Q1/6876 ,  A61K38/00 ,  C07K14/50 ,  C07K14/501 ,  C07K16/22 ,  C07K2317/73 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/75 ,  C12N15/70 ,  C12N15/79 ,  C12Q2600/158

    摘要： Discoveries are disclosed that show particular aspect or recombinant DNA technology can be used successfully to produce hitherto unknown human keratinocyte growth factor (KGF) protein free of other polypeptides. These proteins can be produced in various functional forms from spontaneously secreting cells or from DNA segments introduced into cells. These forms variously enable biochemical and functional studies of this novel protein as well as product of antibodies. Means are described fro determining the level of expression of genes for the KGF protein, for example by measuring mRNA levels in cells or by measuring antigen secreted in extracellular or body fluids.

    摘要翻译： 公开了显示特定方面的发现，或重组DNA技术可以成功地用于产生迄今为止未知的人角质形成细胞生长因子（KGF）蛋白，而不含其他多肽。 这些蛋白质可以从自发分泌的细胞或引入细胞的DNA片段以各种功能形式产生。 这些形式不同地使得这种新型蛋白质的生物化学和功能研究以及抗体的产物。 描述了确定KGF蛋白基因表达水平的方法，例如通过测量细胞中的mRNA水平或通过测量在细胞外或体液中分泌的抗原。