METHOD OF PROLIFERATING PLURIPOTENT STEM CELL
    5.
    发明公开
    METHOD OF PROLIFERATING PLURIPOTENT STEM CELL 有权
    VERFAHREN ZUR PROLIFEATION EINER PLURPOTENTEN STAMMZELLE

    公开(公告)号:EP1734112A1

    公开(公告)日:2006-12-20

    申请号:EP05727500.0

    申请日:2005-03-23

    CPC分类号: C12N5/0606 C12N2533/50

    摘要: A novel growth method is provided for pluripotent stem cells such as ES cells. The method of the invention is a pluripotent stem cell growing method and gene transfer method in which pluripotent stem cells are cultured under conditions that maintain their undifferentiated state and pluripotency, the method being characterized by using a liquid medium and a culturing vessel having immobilized or coated on a substrate solid phase surface a molecule which is adhesive to the pluripotent stem cells in a fixed concentration, to grow the pluripotent stem cells in a dispersed state while maintaining their undifferentiated state and pluripotency, without using feeder cells, or to transfer and express a gene therein.

    摘要翻译: 为多能干细胞如ES细胞提供了一种新的生长方法。 本发明的方法是多能干细胞生长方法和基因转移方法,其中多能干细胞在保持其未分化状态和多能性的条件下培养,该方法的特征在于使用液体培养基和具有固定或涂覆的培养容器 在底物固相表面上以固定浓度与多能干细胞粘合的分子,以分散状态生长多能干细胞,同时保持其未分化状态和多能性,而不使用饲养细胞,或转移和表达 基因。

    METHOD OF GROWING MYOCARDIAL CELLS
    7.
    发明公开
    METHOD OF GROWING MYOCARDIAL CELLS 有权
    VERFAHREN ZUR ANZUCHT VON MYOKARDZELLEN

    公开(公告)号:EP1693451A1

    公开(公告)日:2006-08-23

    申请号:EP04818986.4

    申请日:2004-11-19

    摘要: The proliferation of cardiomyocytes is induced by expressing cyclin and CDK in the cardiomyocytes, and by suppressing the function or action of a Cip/Kip family protein or inhibiting the production of a Cip/Kip family protein. Among the Cip/Kip family proteins, it is preferable to suppress the function of p27 Kip1 or inhibiting the production thereof. As a recombinant vector to be used therefor, there is provided a vector comprising: (1) a cyclin gene; (2) a cyclin-dependent kinase gene; and (3) one or a plurality selected from the group consisting of a gene encoding a factor that inhibits the function or action of a Cip/Kip family protein and a nucleic acid sequence that inhibits the production of Cip/Kip family protein.

    摘要翻译: 通过在心肌细胞中表达细胞周期蛋白和CDK,抑制Cip / Kip家族蛋白的功能或作用或抑制Cip / Kip家族蛋白的产生来诱导心肌细胞的增殖。 在Cip / Kip族蛋白质中,优选抑制p27 Kip1的功能或抑制其生产。 作为用于其的重组载体,提供了载体,其包含:(1)细胞周期蛋白基因; (2)细胞周期蛋白依赖性激酶基因; 和(3)选自由编码抑制Cip / Kip家族蛋白的功能或作用的因子的基因和抑制Cip / Kip家族蛋白的产生的核酸序列组成的组中的一种或多种。