利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

1754 条记录 (耗时 0.035 秒)

    Method for the production of high-level soluble human recombinant interferon alpha in e. coli and vectors useful for such a production
    4.
    发明公开
    Method for the production of high-level soluble human recombinant interferon alpha in e. coli and vectors useful for such a production 审中-公开
    一种用于在大肠杆菌中制备hochlöslichem人重组α干扰素在这种制造中有用的载体的过程

    公开(公告)号：EP2000480A1

    公开(公告)日：2008-12-10

    申请号：EP07290712.4

    申请日：2007-06-07

    申请人： INSTITUT PASTEUR ,  INSTITUT PASTEUR DE TUNIS

    发明人： Fathallah, Mohamed Dahmani ,  Rabhi-Essafi, Imen

    IPC分类号： C07K14/56 ,  C12N15/62

    CPC分类号： C07K14/56 ,  C07K2319/00 ,  C07K2319/23 ,  C07K2319/35 ,  C07K2319/50 ,  C12N9/1088 ,  C12Y205/01018

    摘要： Method for the production of high-level soluble human recombinant interferon alpha protein (rhuIFNα) in E. coli and vectors useful for such a production.
    Said method comprises the steps of:
    (1) Transforming an E. coli selected in the group consisting of E. coli protease deficient host strains, and E. coli reductase deficient host strains, with a recombinant expression vector comprising the sequence encoding the glutathione-S-transferase (GST), a junction sequence including a recognition site for a specific protease and a sequence able to encode an interferon alpha (IFN alpha) protein under the control of an inducible promoter, said vector encoding a GST-IFN alpha fusion protein
    (2) Expressing said interferon alpha protein in conditions comprising the induction of the expression with 0.1 mM-0.5 mM IPTG and a growth temperature of 25° and/or 37°C, depending on said E. coli strain and
    (3) Isolating the expressed IFN alpha protein.

    摘要翻译： 对于在大肠杆菌和寻求生产中有用的载体的生产高级的可溶性重组人干扰素α蛋白（rhuIFN±）方法。 所述方法包括以下步骤：（1）变换为选自由大肠杆菌蛋白酶缺陷宿主菌株在选定的大肠杆菌，和大肠杆菌还原酶缺陷型宿主菌株，用包含编码谷胱甘肽的序列的重组表达载体 S-转移酶（GST），连接序列包含一个识别位点特异性蛋白酶，并且能够编码为干扰素α（IFNα的）蛋白诱导型启动子的控制下的序列，所述载体编码GST-IFNα的融合蛋白 （2）表达所述在包含该表达的诱导用0.1mM-0.5mM的IPTG和25°和/或37℃的生长温度条件C的干扰素α蛋白，取决于所述大肠杆菌菌株，（3）分离所述 表达IFNα蛋白。