摘要:
Easily split enzyme substrates for the quantification of proteases having the general formula wherein
R 1 = H or a protective group, preferably t-butyl-oxycarbonyl, benzyloxycarbonyl; A, - Gly, Ala, Val, Leu, Ile. Ser, Thr, Pro, Pip, Phe or Tyr; A 2 - Arg or Lys; R 2 = an aromatic, possibly substituted, hydrocarbon group, wherein -NH-R 2 is a physico-chemically determinable group, preferably a chromogenic or fluorogenic group which is split by a present enzyme and then forms a cleavage product of the formula H 2 N - R 2 the amount of which can be quantified.
Process for the production of said substrates. Method in the laboratory diagnostics of proteases using said substrates.
摘要:
Hemiron-enriched amino acid preparation consisting of aggregates of hemprotein fragments with hem bonded to histidin groups present in the fragment and a method for preparing this amino acid preparation from hemproteins, especially hemoglobin, wherein the hemprotein in a manner known per se at first is hemolyzed and denaturated and subsequently split with the aid of proteolytic enzymes to the formation of one hemiron-enriched and one fraction which does not contain hemiron and separation of these fractions from each other. The amino acid preparation is suitable for iron enrichment of for instance foodstuffs and for use in pharmaceutical iron preparations.
摘要:
A process for the manufacturing of crumb products comprising preparation of a first raw material flow F1 containing components or part flows f1 - f4, whereof f2 and f4 are optional but preferably present, of which f1 contains liquid milk or milk products such as completely or partly defatted fresh milk, defatted or whole condensed milk, whey, completely or partly desalted whey, butter milk, casein or caseinate, whey protein concentrate or combinations thereof, at a dry solids content of 20-70% by weight, especially 30-45% by weight, suitably at a temperature of about 50°C; f2 contains sugar raw materials in the form of tetroses, pentoses, hexoses such as glucose, fructose, galactose, sugar alcohols such as sorbitol, disaccharides such as lactose, maltose, dextromaltose, partially hydrolyzed starch, syrup products, xylitol or different combinations thereof, suitably in an aqueous solution;
f3 contains dry protein raw materials such as vegetable protein, dry milk solids, whey powder, butter milk powder, which suitably are admixed with water and treated in a colloid mill, at the same dry solids contents as stated for f1; f4 contains one or more amino acids, preferably in combination with one or more reducing sugars, suitably in an aqueous solution; and a second flow F2 consisting of part flows f5 and optionally f6, of which f5 contains fatty components in the form of cream, suitably with a fat content of 12-35% by weight, butter, butter oil, vegetable and/or animal fat products or fat-containing products or mixtures thereof; f6 consists of an emulsifier for the fat phase, which promotes the formation of emulsions of the type oil-in-water, e.g. glyceryl monostearate;
and that the flow F1 at a dry solids content of 20-70% by weight and a pH in the range 4-8, preferably 6.5-7.5, is heat-treated at a temperature of 100-130°C during a period of time of about 7-2 minutes for performing a Maillard reaction, and subsequently is cooled to a temperature of suitably 80-95°C; whereupon the flows F1 at the said temperature and F2 suitably at a temperature of 65-75°C are metered into an emulsifying vessel to the formation of an emulsion of F2 in F1, which emulsion after an optional adjustment of the water content is submitted to a homogenizing treatment in a homogenizer, whereupon the crumb emulsion formed is either used directly or dried to the formation of a powder which optionally can be submitted to a further heat-treatment such as in a fluidized bed for a completing Mailiard reaction, further drying to a water content of suitably 1.5-6° a by weight. especially 2-3.5° by weight, and sieving for obtaining a desired fraction