公开(公告)号：EP2439266B1

公开(公告)日：2017-02-01

申请号：EP11189291.5

申请日：2006-11-28

Applicant: DSM IP Assets B.V.

Inventor： De Swaaf, Maximiliaan Peter Marie ,  Van Dijk, Albertus Alard ,  Edens, Luppo ,  Dekker, Petrus Jacobus Theodorus

IPC: C12N9/16 ,  C12N15/55 ,  A23C9/12 ,  C12N9/62 ,  C12N9/38 ,  A23C19/032 ,  A23C9/127

CPC classification number: A23C9/127 ,  A23C9/1206 ,  A23C19/0328 ,  C12N9/16 ,  C12N9/2402 ,  C12N9/2468 ,  C12N9/2471 ,  C12N9/62 ,  C12Y301/06001 ,  C12Y302/01023 ,  C12Y302/01108

Abstract: The present invention describes an intracellular produced lactase which comprises less than 40 units arylsulfatase activity per NLU of lactase activity. The invention also provides a process comprising treating a substrate with an enzyme preparation, wherein the enzyme preparation is substantially free from arylsulfatase.

公开(公告)号：EP2844751A2

公开(公告)日：2015-03-11

申请号：EP13724535.3

申请日：2013-05-03

Applicant: DSM IP Assets B.V.

Inventor： DEKKER, Petrus Jacobus Theodorus ,  JONG, DE, René Marcel ,  MUIJLWIJK, Cornelis Marinus

IPC: C12N9/64 ,  C12N15/59 ,  A23C19/04 ,  C12N1/19

CPC classification number: C12N9/6483 ,  A23C19/032 ,  A23C19/0326 ,  A23C19/0684 ,  A23C19/072 ,  A23C2220/202 ,  A23C2250/15 ,  C12Y304/23004

Abstract: The invention relates to a polypeptide having chymosin activity which: a. is capable of hydrolysing bovine alpha s1-casein at position F23F24 so as to form αs1-I CN (f24-199) more rapidly than camel chymosin; and b. has a C/P ratio higher than the C/P ratio of bovine chymosin.

Abstract translation: 本发明涉及具有凝乳酶活性的多肽，其：a。 能够在F23F24位水解牛α1酪蛋白，从而比骆驼凝乳酶更快地形成αs1-CN（f24-199）; 和b。 C / P比高于牛乳蛋白的C / P比。

公开(公告)号：EP1954808B1

公开(公告)日：2014-01-01

申请号：EP06819802.7

申请日：2006-11-28

Applicant: DSM IP Assets B.V.

Inventor： SWAAF, DE, Maximiliaan Peter Marie ,  DIJK, VAN, Albertus, Alard ,  EDENS, Luppo ,  DEKKER, Petrus, Jacobus, Theodorus

IPC: A23C9/12 ,  C12N9/38

CPC classification number: A23C9/127 ,  A23C9/1206 ,  A23C19/0328 ,  C12N9/16 ,  C12N9/2402 ,  C12N9/2468 ,  C12N9/2471 ,  C12N9/62 ,  C12Y301/06001 ,  C12Y302/01023 ,  C12Y302/01108

Abstract: The present invention describes an intracellular produced lactase which comprises less than 40 units arylsulfatase activity per NLU of lactase activity. The invention also provides a process comprising treating a substrate with an enzyme preparation, wherein the enzyme preparation is substantially free from arylsulfatase.

公开(公告)号：EP2113027A2

公开(公告)日：2009-11-04

申请号：EP08709070.0

申请日：2008-02-18

Applicant: DSM IP Assets B.V.

Inventor： DIJK, VAN, Albertus, Alard ,  DEKKER, Petrus, Jacobus, Theodorus ,  EFIMOVA, Yulia M.

IPC: C12N9/24

CPC classification number: A23C19/0328 ,  C12N9/2402 ,  C12Y302/01018

Abstract: The present invention relates to an isolated polypeptide which has sialidase activity, selected from the group consisting of: (a) a polypeptide which has an amino acid sequence which has at least 60% amino acid sequence identity with amino acids 1 to 407 of SEQ ID NO: 3; (b) a polypeptide which is encoded by a polynucleotide which hybridizes under low stringency conditions with (i) the nucleic acid sequence of SEQ ID NO: 1 or 2 which is at least 80% or 90% identical over 60, preferably over 100 nucleotides, more preferably at least 90% identical over 200 nucleotides, or (ii) a nucleic acid sequence complementary to the nucleic acid sequence of SEQ ID NO: 1 or 2.

公开(公告)号：EP3077517A1

公开(公告)日：2016-10-12

申请号：EP14843223.0

申请日：2014-12-02

Applicant: DSM IP Assets B.V.

Inventor： JONG, DE, René Marcel ,  DEKKER, Petrus Jacobus Theodorus

IPC: C12N15/81 ,  C07K14/39

CPC classification number: C07K14/39 ,  A23G9/38 ,  A23V2002/00 ,  C12N15/81

Abstract: The present invention relates to a nucleic acid construct comprising: a nucleic acid sequence encoding an ice structuring protein (ISP) comprising the sequence set out in SEQ ID NO: 1 or a sequence at least 80% identical thereto or comprising the sequence set out in amino acids 21 to 261 of SEQ ID NO: 1 or a sequence at least 80% identical thereto; and, linked operably thereto, control sequences permitting expression of the nucleic acid sequence in a filamentous fungal host cell. The construct may be used in a method for the production of an ISP, wherein the ISP is expressed in a filamentous fungal host cell.

Abstract translation: 本发明涉及一种核酸构建体，其包含：编码冰结构化蛋白（ISP）的核酸序列，所述冰结构化蛋白包含SEQ ID NO：1中所示的序列或与其至少80％相同的序列或包含序列 SEQ ID NO：1的氨基酸21至261或与其至少80％相同的序列; 并且与其可操作地连接，允许核酸序列在丝状真菌宿主细胞中表达的控制序列。 该构建体可用于生产ISP的方法中，其中ISP在丝状真菌宿主细胞中表达。

公开(公告)号：EP2126060B1

公开(公告)日：2016-10-05

申请号：EP08717945.3

申请日：2008-03-18

Applicant: DSM IP Assets B.V.

Inventor： DIJK, VAN, Albertus, Alard ,  DEKKER, Petrus, Jacobus, Theodorus ,  WESTERLAKEN, Ilja ,  BAKHUIS, Janna Gardina

IPC: C12N9/06 ,  C12P21/06 ,  C12P21/00 ,  A23J3/08 ,  A23J3/14 ,  A23J3/22

CPC classification number: A23J3/08 ,  A23J3/14 ,  A23J3/22 ,  A23L7/107 ,  A23L29/06 ,  C12N9/0022 ,  C12Y104/03013

公开(公告)号：EP1373491B1

公开(公告)日：2015-12-30

申请号：EP02759791.3

申请日：2002-04-03

Applicant: DSM IP Assets B.V.

Inventor： BECKHOVEN VAN, Rudolf Franciscus Wilhelmus C. ,  PARIDON, VAN, Petrus, Andreas

IPC: A23C9/12 ,  C12N9/38

CPC classification number: A23C9/1206 ,  A23C2210/208 ,  C12N9/2402 ,  C12N9/2471 ,  C12Y302/01023 ,  C12Y302/01108

Abstract: The present invention relates to a lactase solution comprising a lactase solution comprising less than 10 g/kg of poly and oligosaccharides, a process for the production of such a lactase solution from an untreated lactase solution, a sterilized lactase solution and to a process for the production of milk containing sterilized lactase, whereby such lactose is filter sterilized in-line with the milk production process.

公开(公告)号：EP2736351A1

公开(公告)日：2014-06-04

申请号：EP12733111.4

申请日：2012-07-05

Applicant: DSM IP Assets B.V.

Inventor： WNUKOWSKI, Piotr ,  SMOLDERS, Gerardus Johannes Franciscus ,  VEERMAN, Cecile

IPC: A23J1/14 ,  A23J3/14 ,  A23J3/16

CPC classification number: A23J1/14 ,  A23J1/142

Abstract: A process to isolate protein from the meal or oil cake comprising the following steps: • extracting the meal with water to obtain an aqueous solution; • concentrating the aqueous extract to an aqueous solution comprising 5 to 30 wt% protein, preferably 10 to 30 wt% protein; • adding a water-soluble solvent to the concentrated aqueous solution to obtain a protein precipitate; and • separating the protein precipitate from the liquid fraction.

公开(公告)号：EP2439266A3

公开(公告)日：2012-05-09

申请号：EP11189291.5

申请日：2006-11-28

Applicant: DSM IP Assets B.V.

Inventor： De Swaaf, Maximiliaan Peter Marie ,  Van Dijk, Albertus Alard ,  Edens, Luppo ,  Dekker, Petrus Jacobus Theodorus

IPC: C12N9/38 ,  A23C9/12 ,  C12N15/55 ,  C12N9/62

CPC classification number: A23C9/127 ,  A23C9/1206 ,  A23C19/0328 ,  C12N9/16 ,  C12N9/2402 ,  C12N9/2468 ,  C12N9/2471 ,  C12N9/62 ,  C12Y301/06001 ,  C12Y302/01023 ,  C12Y302/01108

Abstract: The present invention describes an intracellular produced lactase which comprises less than 40 units arylsulfatase activity per NLU of lactase activity. The invention also provides a process comprising treating a substrate with an enzyme preparation, wherein the enzyme preparation is substantially free from arylsulfatase.

公开(公告)号：EP2004820A1

公开(公告)日：2008-12-24

申请号：EP07728023.8

申请日：2007-04-12

Applicant: DSM IP Assets B.V.

Inventor： HAAN, DE, André ,  CAUSSETTE, Mylene ,  SCHOONEVELD-BERGMANS, Margot, Elisabeth, Francoise

IPC: C12N9/96

CPC classification number: C12N9/96 ,  A23C19/0326 ,  C12N9/6413 ,  C12N9/6478 ,  C12N9/6483 ,  C12Y304/00 ,  C12Y304/23004

Abstract: The present invention provides a liquid composition comprising: (i) an aspartic protease; and (ii) an inorganic salt and/or a polyalcohol; in which composition; the sum concentration of sorbate, benzoate and alkyl esters of para- hydroxybenzoate is less than 0.010 mol/l; the standard plate count