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    Medium for plant protoplast culture
    2.
    发明公开
    Medium for plant protoplast culture 失效
    中等植物原生质体培养。

    公开(公告)号：EP0176637A1

    公开(公告)日：1986-04-09

    申请号：EP84306699.4

    申请日：1984-10-01

    申请人： AGRACETUS

    发明人： Yang, Ning-Sun

    IPC分类号： C12N15/00 ,  C12N5/00

    CPC分类号： C12N5/0025 ,  A01H4/00 ,  C12N5/14 ,  Y10S435/948

    摘要： A culture medium and method for the isolation and culture of plant protoplasts is disclosed which includes in it a quantity of neutral mammalian blood serum. An addition of 1 to 25% serum to protoplast media results in a greater yield of viable protoplast cells, helps to protect the protoplasts in culture, and makes it possiible to obtain and maintain corn protoplasts, a heretofore difficult task.

    GAS DRIVEN GENE DELIVERY INSTRUMENT
    3.
    发明公开
    GAS DRIVEN GENE DELIVERY INSTRUMENT 失效
    气体驱动ELEMENT FOR遗传物质的充放电

    公开(公告)号：EP0690732A4

    公开(公告)日：1996-06-12

    申请号：EP95909280

    申请日：1995-01-20

    申请人： AGRACETUS

    发明人： MCCABE DENNIS E

    IPC分类号： C12N15/09 ,  A61K48/00 ,  A61M5/20 ,  A61M5/28 ,  A61M5/30 ,  A61M5/307 ,  A61M5/31 ,  C12M1/00 ,  C12M3/00 ,  C12N15/63

    CPC分类号： A61M5/3015 ,  A61M5/2053 ,  A61M2005/005 ,  C12M35/00

    摘要： A gas driven apparatus (10) for accelerating particles (16) coated with a genetic material into a target, comprises a reservoir (12) for releasably retaining a gas at a sufficiently high-pressure to detach the particles from the surface of a sample cartridge (14) and to carry the particles through the apparatus (10) toward the target. When leaving the apparatus (10), the particles (16) entrained in the gas stream pass through a substantially conical exit nozzle (24) which causes the pattern of distribution of the particles to greatly expand. Methods for using the apparatus and for preparing the sample cartridges (14) are also described.

    TRANSGENIC COTTON PLANTS PRODUCING HETEROLOGOUS BIOPLASTIC
    4.
    发明公开
    TRANSGENIC COTTON PLANTS PRODUCING HETEROLOGOUS BIOPLASTIC 失效
    异种Biokunstoff生产转基因棉花。

    公开(公告)号：EP0625006A1

    公开(公告)日：1994-11-23

    申请号：EP94902371.0

    申请日：1993-11-19

    申请人： AGRACETUS, INC.

    发明人： MALIYAKAL, John

    IPC分类号： A01H5 ,  C12N9 ,  C12N15 ,  C12P7

    CPC分类号： C12N9/0006 ,  A01H5/10 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/8243 ,  C12P7/625

    摘要： L'invention concerne une plante productrice de fibres comprenant dans son génome une construction génétique hétérologue. Cette construction génétique comprend un promoteur spécifique aux fibres et une séquence codante sélectionnée à partir du groupe constitué par des séquences codant des gènes producteurs de bioplastiques. De préférence, la séquence codante est sélectionnée à partir du groupe constitué par cétothiolase, réductase de acéto-acétyl-CoA et synthase d'acide poly-bêta-hydroxybutyrique. L'invention concerne également des semences de la plante contenant cette construction génétique, ainsi que des cellules de plantes contenant ladite construction.

    Plant transformation process with early identificaton of germ line transformation events
    9.
    发明公开
    Plant transformation process with early identificaton of germ line transformation events 失效
    弗朗西斯科·冯·基恩巴恩转型革命。

    公开(公告)号：EP0444882A2

    公开(公告)日：1991-09-04

    申请号：EP91301545.9

    申请日：1991-02-26

    申请人： AGRACETUS, INC.

    发明人： Christou, Paul ,  McCabe, Dennis E.

    IPC分类号： C12N15/82 ,  C12N15/87

    CPC分类号： C12N15/8207

    摘要： A method is disclosed for making more efficient the particle-mediated germ line genetic transformation of bean species such as soybean. After a particle-mediated transformation event, in the absence of a selectable marker gene, relatively large numbers of plants must be regenerated to find the relatively low likelihood germ line transformation events which have occurred. It has been discovered that using in the transformation process a marker gene linked to the gene of interest, and by excising a segment of the stem of the shoot during the regeneration process and assaying the sequent for the marker gene, certain patterns or phenotypes can be identified in the stem segment which are associated with an increased frequency of germ line transformation events. As the plants are regenerated, other indices of gene expression, at the first trifoliate leaf stage and at the third or fourth trifoliate leaf stage, also serve as markers of the likelihood of germ line transformation. By using these markers in the relatively early stages of plant regeneration to assay for likelihood of germ line events, it is possible to concentrate regeneration efforts on plants most likely to yield germ line events, and to discard the others, so as to lower the burden and effort in achieving a desired number of transformation events.

    摘要翻译： 公开了一种使豆类如大豆的颗粒介导的种系遗传转化更有效的方法。 在颗粒介导的转化事件之后，在没有选择性标记基因的情况下，必须再生相对大量的植物以找到发生的相对较低的可能性种系转化事件。 已经发现，在转化过程中使用与感兴趣的基因连锁的标记基因，并且在再生过程中切除芽的茎段并测定标记基因的后续序列，某些模式或表型可以是 在茎段中鉴定，其与种系转化事件的频率增加相关。 当植物再生时，其他基因表达指数，在第一叶片叶片阶段和第三或第四叶片叶片阶段，也可作为种系转化的可能性的标记。 通过在植物再生的相对早期阶段使用这些标记物来测定种系事件的可能性，可以将再生努力集中在最有可能产生种系事件的植物上，并丢弃其它物质，从而降低其负担 并努力实现所需数量的转型事件。