公开(公告)号：EP1809733B1

公开(公告)日：2015-01-07

申请号：EP04794243.8

申请日：2004-10-06

申请人： Agentase, LLC

发明人： LEJEUNE, Keith, E. ,  MYSLIWCZYK, Richard, J. ,  HOLZAPFEL, Paul, L. ,  ERBELDINGER, Markus

IPC分类号： C12M1/34 ,  C12Q1/34 ,  C12Q1/46

CPC分类号： G01N21/80 ,  C12Q1/001 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/46 ,  G01N1/2035 ,  G01N1/2247 ,  G01N33/0031 ,  G01N33/0062 ,  G01N35/00871 ,  G01N35/1095

公开(公告)号：EP1928560A2

公开(公告)日：2008-06-11

申请号：EP06851338.1

申请日：2006-04-06

申请人： Verenium Corporation ,  Agentase, LLC ,  Life Science Research Israel Ltd.

发明人： ROBERTSON, Dan, E. ,  RICHARDSON, Toby ,  KUSTEDJO, Karen ,  AMITAI, Gabriel ,  LEJEUNE, Keith ,  BERBERICH, Jason ,  CHAPLIN, Jennifer, Ann ,  SINCLAIR, Jessica

IPC分类号： A62D3/02 ,  A62D101/04

CPC分类号： A62D3/02 ,  A62D2101/02 ,  C12N9/00 ,  C12N9/16

摘要： The invention provides novel enzymes and methods for enzyme-based active decontamination, e.g., nerve agent detoxification. In one aspect, the invention provides enzymes having non-heme chloroperoxidase (nhCPOs) activity, and methods for bleaching and degradation of lignin. The invention provides polypeptides, nucleic acids, infective vehicles, transduced or infected cells or plants and/or transgenic plants to, and methods of using them, e.g., provide self-protecting, pesticide-resistant and/or herbicide-resistant cells or plants, where in one aspect the polypeptides of the invention act to hydrolyzing P-S or P-F bonds and detoxify acetylcholinesterases or butyrylcholinesterases. In one aspect, the invention provides an enzyme mixture or composition that provides broad-spectrum, agent-specific oxidation and hydrolysis activities as well as hypohalite generation for oxidation or hydrolysis of V-agents, G-agents and H-agents and which generates oxidants such as chlorine dioxide and reactive radicals for killing of biological agents, including anthrax spores.

公开(公告)号：EP1543051B1

公开(公告)日：2011-11-02

申请号：EP03784929.6

申请日：2003-08-06

申请人： Agentase, LLC

发明人： LEJEUNE, Keith, E. ,  ALLINSON, Bryan

IPC分类号： C12N11/08 ,  C08G18/10 ,  C08G18/64

CPC分类号： C12N11/08 ,  C08G18/10 ,  C08G18/6446 ,  C08G18/64 ,  C08G18/302

摘要： A polyurethane polymer includes at least one buffer selected to adjust pH to a pH within a desired range. The buffer compound is immobilized within the polymer, and the polymer has a buffer capacity in excess of 3 micromoles of acid or base per gram polymer. A polymer includes at least one enzyme that is selected to catalyze a reaction of a substance. The enzyme is immobilized within the polymer. The polymer also includes at least one buffer selected to adjust the pH in the vicinity of the enzyme to a pH within a desired range. The buffer compound is also immobilized within the polymer. The polymer preferably has enzyme activity and a buffer capacity in excess of 3 micromoles acids or base per gram polymer. In one embodiment, each of the enzymes(s) and the buffer(s) are covalently bonded to the polymer.

公开(公告)号：EP2153220A2

公开(公告)日：2010-02-17

申请号：EP08826547.5

申请日：2008-04-21

申请人： Agentase, LLC

发明人： WALKER, Jeremy, P. ,  WASHBURN, Jon, M., Jr. ,  ERBELDINGER, Markus

IPC分类号： G01N33/00

CPC分类号： G01N21/78 ,  Y10T436/172307

摘要： A device for continuous detection of the presence of a cyanide analyte and/or cyanogen analyte in an aqueous sample which relies upon continuous sampling and controlled delivery of reagents for a chemical reaction which forms a colored dye in the presence of the analyte(s). The device employs a single chemical detection pathway which detects both cyanide and cyanogen and demonstrates continuous user- free operational stability over at least a one month period of time. The continuous monitoring device optionally may include a command post computer interface for enabling the remote monitoring of one or more devices, a wireless communication module for providing real-time data monitoring of the devices to a central monitoring facility, and a global positioning system module to enable the determination of an exact location of the analyte contamination in a water network. A method for detecting these analytes is also provided.

公开(公告)号：EP1543051A4

公开(公告)日：2009-08-12

申请号：EP03784929

申请日：2003-08-06

申请人： AGENTASE LLC

发明人： LEJEUNE KEITH E ,  ALLINSON BRYAN

IPC分类号： C12N11/08 ,  C08G18/10 ,  C08G18/64

CPC分类号： C12N11/08 ,  C08G18/10 ,  C08G18/6446 ,  C08G18/64 ,  C08G18/302

摘要： A polyurethane polymer includes at least one buffer selected to adjust pH to a pH within a desired range. The buffer compound is immobilized within the polymer, and the polymer has a buffer capacity in excess of 3 micromoles of acid or base per gram polymer. A polymer includes at least one enzyme that is selected to catalyze a reaction of a substance. The enzyme is immobilized within the polymer. The polymer also includes at least one buffer selected to adjust the pH in the vicinity of the enzyme to a pH within a desired range. The buffer compound is also immobilized within the polymer. The polymer preferably has enzyme activity and a buffer capacity in excess of 3 micromoles acids or base per gram polymer. In one embodiment, each of the enzymes(s) and the buffer(s) are covalently bonded to the polymer.

公开(公告)号：EP1809733A2

公开(公告)日：2007-07-25

申请号：EP04794243.8

申请日：2004-10-06

申请人： Agentase, LLC

发明人： LEJEUNE, Keith, E. ,  MYSLIWCZYK, Richard, J. ,  HOLZAPFEL, Paul, L. ,  ERBELDINGER, Markus

IPC分类号： C12M1/34 ,  C12Q1/34 ,  C12Q1/46

CPC分类号： G01N21/80 ,  C12Q1/001 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/46 ,  G01N1/2035 ,  G01N1/2247 ,  G01N33/0031 ,  G01N33/0062 ,  G01N35/00871 ,  G01N35/1095

摘要： A sensor for the intermittent or continuous detection of the presence of at least one analyte in an environmental sample includes at least one enzyme that is selected to either (i) catalyze a reaction of the analyte to chemically convert the analyte to a product compound or (ii) be inhibited by the analyte in the presence of a substrate compound. The sensor also includes at least one sensor for monitoring or at least one indicator compound selected to produce a measurable change of state as a result of the interaction of the analyte and the enzyme. Optionally, each of the enzyme and the indicator compound are incorporated within a single polymer.

公开(公告)号：EP1809733A4

公开(公告)日：2010-04-21

申请号：EP04794243

申请日：2004-10-06

申请人： AGENTASE LLC

发明人： LEJEUNE KEITH E ,  MYSLIWCZYK RICHARD J ,  HOLZAPFEL PAUL L ,  ERBELDINGER MARKUS

IPC分类号： C12M1/34 ,  C12Q1/34 ,  C12Q1/46

CPC分类号： G01N21/80 ,  C12Q1/001 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/46 ,  G01N1/2035 ,  G01N1/2247 ,  G01N33/0031 ,  G01N33/0062 ,  G01N35/00871 ,  G01N35/1095

公开(公告)号：EP1234051A1

公开(公告)日：2002-08-28

申请号：EP00990989.6

申请日：2000-11-10

申请人： Agentase, LLC

发明人： LEJEUNE, Keith, E. ,  RUSSELL, Alan, J.

IPC分类号： C12Q1/34 ,  C12M1/00

CPC分类号： C12Q1/58 ,  C12Q1/34 ,  C12Q1/46 ,  G01N33/521

摘要： A sensor for detecting the presence of at least one analyte includes at least one enzyme that is selected to either (i) catalyze a reaction of the analyte to chemically convert the analyte to a product compound or (ii) be inhibited by the analyte in the presence of a substrate compound. The sensor also includes at least one indicator compound selected to produce a measurable change of state as a result of the interaction of the analyte and the enzyme. Each of the enzyme and the indicator compound are incorporated within a single polymer. The figure illustrates an embodiment of a reaction schematic for biopolymer synthesis.

公开(公告)号：EP2861267A2

公开(公告)日：2015-04-22

申请号：EP13804843.4

申请日：2013-06-12

申请人： Agentase, LLC

发明人： LEECH, Anna M. ,  WALKER, Jeremy P. ,  DONAHUE, Scott ,  JOHNSON, Jermaine D. ,  SINCLAIR, Jessica J.

IPC分类号： A61L15/38 ,  A61L15/60 ,  C12Q1/26

CPC分类号： A61L15/38 ,  A61F13/00059 ,  A61F13/00063 ,  A61K9/06 ,  A61K9/7007 ,  A61K38/443 ,  A61L15/26 ,  A61L15/425 ,  A61L15/44 ,  A61L15/46 ,  A61L2300/404 ,  A61L2300/434 ,  A61L2420/06 ,  C08L75/04

摘要： A smart antimicrobial material and dressing to inhibit microbial growth is provided. Endogenous chemicals, such as metabolites produced from bacteria are utilized as chemical substrates and converted by enzymes to produce a disinfecting compound that will in turn inhibit the targeted microorganism. The material shall remain passive until such time as it encounters a microbe which expresses and/or secretes specific metabolites or markers. The enzyme or enzymes embedded in the smart material converts the metabolite into a disinfecting compound, which in turn either kills the microorganism or prevents it from multiplying on the surface of the material.

公开(公告)号：EP1234051B1

公开(公告)日：2008-08-13

申请号：EP00990989.6

申请日：2000-11-10

申请人： Agentase, LLC

发明人： LEJEUNE, Keith, E. ,  RUSSELL, Alan, J.

IPC分类号： C12Q1/34 ,  C12M1/00

CPC分类号： C12Q1/58 ,  C12Q1/34 ,  C12Q1/46 ,  G01N33/521

摘要： A sensor for detecting the presence of at least one analyte includes at least one enzyme that is selected to either (i) catalyze a reaction of the analyte to chemically convert the analyte to a product compound or (ii) be inhibited by the analyte in the presence of a substrate compound. The sensor also includes at least one indicator compound selected to produce a measurable change of state as a result of the interaction of the analyte and the enzyme. Each of the enzyme and the indicator compound are incorporated within a single polymer. The figure illustrates an embodiment of a reaction schematic for biopolymer synthesis.