公开(公告)号：EP1902126A1

公开(公告)日：2008-03-26

申请号：EP06787366.1

申请日：2006-07-12

申请人： CODON DEVICES, INC

发明人： BAYNES, Brian, M.

IPC分类号： C12N1/00

CPC分类号： C12N15/1086 ,  C12N15/1093 ,  C40B30/06

摘要： Provided herein are compositions and methods for metabolic pathway engineering. The methods involve combining two or more cells expressing potential pathway proteins extracellularly in the presence of reactants. Also provided are librarie of cells expressing a plurality of pathway components and/or a plurality of variants of a given pathway component extracellularly.

公开(公告)号：EP1812598A1

公开(公告)日：2007-08-01

申请号：EP05815025.1

申请日：2005-10-18

申请人： Codon Devices, Inc.

发明人： AFEYAN, Noubar ,  CHURCH, George ,  JACOBSON, Joseph ,  BAYNES, Brian, M. ,  NESMITH, Kenneth, Gabriel ,  CHAPMAN, Brad, Alan ,  STRACK-LOGUE, Bettina

IPC分类号： C12Q1/68

CPC分类号： C12N15/66 ,  C12N15/10 ,  C12Q1/6844 ,  C12Q2531/113 ,  C12Q2521/514 ,  C12Q2533/107 ,  C12Q2521/307 ,  C12Q2533/101

摘要： Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of nucleic acid polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments, the methods involve pre­amplification of one or more oligonucleotides using 'universal' primers, reduction of the error rate in oligonucleotide and/or nucleic acid products, and sequence optimization and oligonucleotides design. Also provided are low-purity arrays of nucleic acids and methods for assembling nucleic acids using oligonucleotides obtained from low-purity arrays.

公开(公告)号：EP1848801A1

公开(公告)日：2007-10-31

申请号：EP06718493.7

申请日：2006-01-13

申请人： Codon Devices, Inc.

发明人： CHURCH, George ,  BAYNES, Brian

IPC分类号： C12N15/10 ,  C12Q1/68

CPC分类号： C12N15/1089 ,  C12N15/1031 ,  C12N15/66 ,  C40B30/02 ,  G06F19/16 ,  G06F19/22

摘要： In certain aspects the present invention provides methods and compositions related to rational protein design.

公开(公告)号：EP2155868A2

公开(公告)日：2010-02-24

申请号：EP08743057.5

申请日：2008-04-19

申请人： CODON DEVICES, INC

发明人： LIPPOW, Shaun ,  LIPOVSEK, Dasa ,  AHA, Patricia, M.

IPC分类号： C12N9/22

CPC分类号： C12N9/22 ,  C12N15/62

摘要： Aspects of the invention provide engineered endonucleases that are characterized by both a long recognition sequence and specific cleavage outside of the recognition site. Engineered endonucleases of the invention are useful for manipulating long pieces of DNA.

公开(公告)号：EP2137308A2

公开(公告)日：2009-12-30

申请号：EP08727167.2

申请日：2008-03-26

申请人： CODON DEVICES, INC

发明人： RAKESTRAW, James, A. ,  LIPOVSEK, Dasa

IPC分类号： C12N15/10 ,  C40B40/02

CPC分类号： G01N33/566 ,  C07K2319/035 ,  C12N15/1037 ,  C40B10/00 ,  C40B30/04 ,  C40B40/02 ,  C40B40/08 ,  G01N2333/705

摘要： Aspects of the invention provide compositions and methods for displaying engineered polypeptides on a cell surface. According to aspects of the invention, immobilized polypeptides can be screened to identify one or more variants having one or more functional or structural properties of interest. Aspects of the invention provide composition and methods for producing engineered protein or protein variants having a functional or a structural property of interest.

公开(公告)号：EP2078077A2

公开(公告)日：2009-07-15

申请号：EP07839344.4

申请日：2007-10-04

申请人： CODON DEVICES, INC

发明人： BAYNES, Brian M. ,  DANNER, John P. ,  LIPOVSEK, Dasa ,  BASU, Subhayu

IPC分类号： C12N15/10

CPC分类号： C12N15/1027 ,  C12N15/1034 ,  C12N15/1093 ,  C12N15/64 ,  C12N15/66 ,  C40B40/06 ,  C40B40/08 ,  C40B50/08

摘要： Aspects of the invention relate to the design and synthesis of nucleic acid libraries containing non-random mutations or variants. Aspects of the invention provide methods for assembling libraries containing high densities of predetermined variant sequences. Certain embodiments relate to the design and synthesis of nucleic acid libraries that express a predetermined polypeptide from a library of nucleic acids having silent sequence variants. Certain embodiments relate to the design and synthesis of nucleic acid libraries that express predetermined RNA variants that encode the same polypeptide sequence.