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1.发明公开IMPROVED METHODS FOR MAKING AND USING POLYNUCLEOTIDE SEQUENCES IN THE SYNTHESIS OF ALKALOID COMPOUNDS 审中-公开改进方法生物碱合成的制备和核苷酸的使用
公开(公告)号：EP3137618A1
公开(公告)日：2017-03-08
申请号：EP15785253.4
申请日：2015-04-27
申请人： Epimeron Inc.
IPC分类号： C12P17/12 , C12N15/00 , C12N15/52 , C12N15/54 , C12N15/62 , C12N15/63 , C12N13/00 , C12P13/00 , C12P17/00 , C12Q1/68 , C40B20/00 , C40B30/00
CPC分类号： C12P17/12 , C12N15/1082 , C12N15/1096 , C12N15/63 , C12Y201/01 , C40B10/00 , C40B20/00
摘要： Novel methods that may be used for the manufacture of plant alkaloid compounds and novel polynucleotide compounds are provided. The plant alkaloid compounds are useful as medicinal compounds.
摘要翻译： 新方法也可用于植物生物碱化合物和新的多核苷酸的化合物的制造中提供。 所述植物生物碱化合物用作药用化合物是有用的。
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2.发明公开NEGATIVE SELECTION AND STRINGENCY MODULATION IN CONTINUOUS EVOLUTION SYSTEMS 有权负选择而产生STRINGENZMODULATION不断发展中，系统
公开(公告)号：EP3097196A2
公开(公告)日：2016-11-30
申请号：EP15759185.0
申请日：2015-01-20
CPC分类号： C12N15/1058 , C12N7/00 , C12N15/01 , C12N15/1024 , C12N15/1037 , C12N15/64 , C12N15/70 , C12N2795/14121 , C12N2795/14152 , C40B10/00 , C40B50/06
摘要： Strategies, systems, methods, reagents, and kits for phage-assisted continuous evolution are provided herein. These include strategies, systems, methods, reagents, and kits allowing for stringency modulation to evolve weakly active or inactive biomolecule variants, negative selection of undesired properties, and/or positive selection of desired properties.
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公开(公告)号：EP2137308A2
公开(公告)日：2009-12-30
申请号：EP08727167.2
申请日：2008-03-26
申请人： CODON DEVICES, INC
CPC分类号： G01N33/566 , C07K2319/035 , C12N15/1037 , C40B10/00 , C40B30/04 , C40B40/02 , C40B40/08 , G01N2333/705
摘要： Aspects of the invention provide compositions and methods for displaying engineered polypeptides on a cell surface. According to aspects of the invention, immobilized polypeptides can be screened to identify one or more variants having one or more functional or structural properties of interest. Aspects of the invention provide composition and methods for producing engineered protein or protein variants having a functional or a structural property of interest.
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公开(公告)号：EP1268801A2
公开(公告)日：2003-01-02
申请号：EP01925538.9
申请日：2001-04-04
发明人： OHLIN, Mats , SODERLIND, Eskil , CARLSSON, Roland
CPC分类号： C12N15/1058 , C07K16/00 , C07K2317/56 , C07K2317/565 , C07K2317/567 , C07K2317/622 , C40B10/00
摘要： The present invention provides a method for producing a polynucleotide sequence encoding an antibody variable domain, the variable domain comprising complementarity-determining regions (CDRs) located within a selected framework (the 'master framework'), the method comprising the steps of (a) providing at least one nucleic acid molecule encoding one or more CDRs and associated framework regions (the 'original framework'), (b) amplifying at least one CDR-encoding portion of the nucleic acid molecule(s) of step (a) using one or more pairs of oligonucleotides as amplification primers and (c) assembling a polynucleotide sequence encoding an antibody variable domain by combining the amplified CDR-encoding nucleotide sequences produced in step (b) with nucleotide sequences encoding said master framework, wherein the oligonucleotide primers of step (b) comprise nucleotide sequences which differ from the corresponding nucleotide sequences encoding said master framework. The invention further provides an antibody library, such as a phage display library, and methods of making the same.
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公开(公告)号：EP4421812A2
公开(公告)日：2024-08-28
申请号：EP24160629.2
申请日：2015-11-25
申请人： Biolojic Design Ltd.
发明人： OFRAN, Yanay , NIMROD, Guy , FISCHMAN, Sharon , HERMAN, Asael
IPC分类号： G16B35/20
CPC分类号： C40B10/00 , G01N33/6878 , C07K2317/56520130101 , G16B20/00 , G16B15/00 , G16B35/00 , G16C20/60 , G16B35/20 , G16B20/30 , G16B15/30 , G16B20/50
摘要： A library comprising variants of an antigen-binding template molecule, wherein (a) the antigen-binding template molecule does not specifically bind the epitope of interest but is known to specifically bind another epitope; (b) the antigen-binding template molecule has at least one of the following characteristics: (i) a predicted shape complementarity to the epitope of interest, (ii) a predicted physico-chemical complementarity to the epitope of interest, wherein it is predicted that upon mutation at least one amino acid residue position within the antigen-binding template molecule forms a chemical bond with an atom in the epitope of interest, or (iii) a predicted free energy of the interaction with the epitope of interest, as determined by computational methods such as but not limited to protein docking or free energy calculations; and (c) the variants have at least one residue comprising a substitution of an amino acid residue compared to the amino acid residue present in the antigen-binding template molecule.
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公开(公告)号：EP2137308B1
公开(公告)日：2016-08-03
申请号：EP08727167.2
申请日：2008-03-26
申请人： Agenus Inc.
CPC分类号： G01N33/566 , C07K2319/035 , C12N15/1037 , C40B10/00 , C40B30/04 , C40B40/02 , C40B40/08 , G01N2333/705
摘要： Aspects of the invention provide compositions and methods for displaying engineered polypeptides on a cell surface. According to aspects of the invention, immobilized polypeptides can be screened to identify one or more variants having one or more functional or structural properties of interest. Aspects of the invention provide composition and methods for producing engineered protein or protein variants having a functional or a structural property of interest.
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7.发明授权METHOD FOR MODULATING THE EVOLUTION OF A POLYPEPTIDE ENCODED BY A NUCLEIC ACID SEQUENCE 有权方法用于调制的同位素标记序列编码多肽发展的
公开(公告)号：EP1799823B1
公开(公告)日：2009-11-25
申请号：EP05850658.5
申请日：2005-09-19
申请人： INSTITUT PASTEUR
发明人： MAZEL, Didier , CAMBRAY, Guillaume
CPC分类号： C12N9/003 , C12N15/01 , C12N15/102 , C12N15/1058 , C40B10/00 , G06F19/22 , Y10T436/143333
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公开(公告)号：EP1268801B1
公开(公告)日：2004-06-23
申请号：EP01925538.9
申请日：2001-04-04
发明人： OHLIN, Mats , SODERLIND, Eskil , CARLSSON, Roland
CPC分类号： C12N15/1058 , C07K16/00 , C07K2317/56 , C07K2317/565 , C07K2317/567 , C07K2317/622 , C40B10/00
摘要： The present invention provides a method for producing a polynucleotide sequence encoding an antibody variable domain, the variable domain comprising complementarity-determining regions (CDRs) located within a selected framework (the 'master framework'), the method comprising the steps of (a) providing at least one nucleic acid molecule encoding one or more CDRs and associated framework regions (the 'original framework'), (b) amplifying at least one CDR-encoding portion of the nucleic acid molecule(s) of step (a) using one or more pairs of oligonucleotides as amplification primers and (c) assembling a polynucleotide sequence encoding an antibody variable domain by combining the amplified CDR-encoding nucleotide sequences produced in step (b) with nucleotide sequences encoding said master framework, wherein the oligonucleotide primers of step (b) comprise nucleotide sequences which differ from the corresponding nucleotide sequences encoding said master framework. The invention further provides an antibody library, such as a phage display library, and methods of making the same.
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9.发明公开
公开(公告)号：EP3384075A1
公开(公告)日：2018-10-10
申请号：EP16870141.5
申请日：2016-11-18
发明人： RAGHAVAN, Varadarajan , SAHOO, Anusmita , KHARE, Shruti , JAIN, Pankaj , AHMED, Shahbaz , GUPTA, Kritika
CPC分类号： C12N15/10 , C12N15/1058 , C40B10/00 , C40B20/00
摘要： The present disclosure relates to a method of protein structure and amino acid residue interaction prediction based on saturation suppressor mutagenesis screening of a protein of interest. The method of the instant disclosure can be adapted for multi- protein complexes, and is useful where crystal structure of a protein of interest is not available.
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10.发明授权NEW MICRO-ORGANISMS FOR THE PRODUCTION OF 1,2-PROPANEDIOL OBTAINED BY A COMBINATION OF EVOLUTION AND RATIONAL DESIGN. 有权新的微生物为1,2-丙二醇的生产过程中获得按组合演化与合理设计
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