公开(公告)号：EP3461894B1

公开(公告)日：2020-05-13

申请号：EP18200117.2

申请日：2016-08-05

申请人： Caribou Biosciences, Inc.

发明人： DONOHOUE, Paul, Daniel ,  MAY, Andrew, Paul

IPC分类号： C12N15/11 ,  C12N15/113 ,  C12N9/22 ,  C12N9/96

公开(公告)号：EP3320092B1

公开(公告)日：2018-10-17

申请号：EP16753538.4

申请日：2016-08-05

申请人： Caribou Biosciences, Inc.

发明人： DONOHOUE, Paul, Daniel ,  MAY, Andrew, Paul

IPC分类号： C12N15/11 ,  C12N15/113

CPC分类号： C12N15/87 ,  C12N9/22 ,  C12N9/96 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/3513 ,  C12N2310/531

摘要： The present specification discloses engineered Type II CRISPR-Cas9 systems comprising split-nexus Cas9-associated polynucleotides (sn-casPNs), including systems comprising three split-nexus Cas9-associated polynucleotides (sn1-casPN/sn2-casPN/sn3-casPN) and systems comprising two split-nexus Cas9-associated polynucleotides (sn1-casPN/sn2-casPN). Together with a Cas9 protein, the sn-casPNs facilitate site-specific modifications, including cleavage and mutagenesis, of a target polynucleotide in vitro and in vivo. Furthermore, the engineered Type II CRISPR-Cas9 systems comprising sn-casPNs are useful in methods of regulating expression of a target nucleic acid. Methods are described herein for the creation of a variety of engineered Type II CRISPR-Cas9 systems comprising two or more sn-casPNs. Polynucleotide sequences, expression cassettes, vectors, compositions, and kits for carrying out a variety of methods are also described. Furthermore, the present specification provides genetically modified cells, compositions of modified cells, transgenic organisms, pharmaceutical compositions, as well as a variety of compositions and methods involving the engineered Type II CRISPR-Cas9 systems.

公开(公告)号：EP3320092A1

公开(公告)日：2018-05-16

申请号：EP16753538.4

申请日：2016-08-05

申请人： Caribou Biosciences, Inc.

发明人： DONOHOUE, Paul, Daniel ,  MAY, Andrew, Paul

IPC分类号： C12N15/11 ,  C12N15/09 ,  C12N15/63

CPC分类号： C12N15/87 ,  C12N9/22 ,  C12N9/96 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/10 ,  C12N2310/20 ,  C12N2310/3513 ,  C12N2310/531

摘要： The present specification discloses engineered Type II CRISPR-Cas9 systems comprising split-nexus Cas9-associated polynucleotides (sn-casPNs), including systems comprising three split-nexus Cas9-associated polynucleotides (sn1-casPN/sn2-casPN/sn3-casPN) and systems comprising two split-nexus Cas9-associated polynucleotides (sn1-casPN/sn2-casPN). Together with a Cas9 protein, the sn-casPNs facilitate site-specific modifications, including cleavage and mutagenesis, of a target polynucleotide in vitro and in vivo. Furthermore, the engineered Type II CRISPR-Cas9 systems comprising sn-casPNs are useful in methods of regulating expression of a target nucleic acid. Methods are described herein for the creation of a variety of engineered Type II CRISPR-Cas9 systems comprising two or more sn-casPNs. Polynucleotide sequences, expression cassettes, vectors, compositions, and kits for carrying out a variety of methods are also described. Furthermore, the present specification provides genetically modified cells, compositions of modified cells, transgenic organisms, pharmaceutical compositions, as well as a variety of compositions and methods involving the engineered Type II CRISPR-Cas9 systems.

公开(公告)号：EP2984175A1

公开(公告)日：2016-02-17

申请号：EP14800396.5

申请日：2014-04-11

申请人： Caribou Biosciences, Inc.

发明人： VAN DER OOST, John ,  SWARTS, Daniël, Christianus ,  MAY, Andrew, Paul ,  HAURWITZ, Rachel, E.

IPC分类号： C12Q1/00

CPC分类号： C12N15/902 ,  C12N15/10 ,  C12N15/102 ,  G01N33/542

摘要： This disclosure provides for compositions and methods for the use of designed nucleic acid-targeting nucleic acids, Argonautes, and complexes thereof.

摘要翻译： 本公开提供了用于使用设计的核酸靶向核酸，Argonautes及其复合物的组合物和方法。 在一个方面，本公开提供了一种组合物，其包含：复合物，其包含：Argonaute和设计的靶向核酸的核酸; 和靶核酸，其中所述设计的靶向核酸的核酸与靶核酸杂交。 在一些实施方案中，靶核酸是双链的。 在一些实施方案中，Argonaute与原核Argonaute包含至少30％的氨基酸同一性。 在一些实施方案中，Argonaute与细菌Argonaute包含至少30％的氨基酸同一性。 在一些实施方案中，Argonaute与古菌Argonaute具有至少30％的氨基酸同一性。 在一些实施方案中，Argonaute与来自嗜中温的Argonaute具有至少30％的氨基酸同一性。 在一些实施方案中，Argonaute与来自嗜热生物的Argonaute具有至少30％的氨基酸同一性。

公开(公告)号：EP4423502A1

公开(公告)日：2024-09-04

申请号：EP22809301.9

申请日：2022-10-24

申请人： Caribou Biosciences, Inc.

发明人： SMITH, Stephen

IPC分类号： G01N33/542 ,  C12Q1/25 ,  C12Q1/6823

CPC分类号： C12Q1/6823 ,  G01N33/542 ,  G01N2333/92220130101 ,  C12Q1/34

公开(公告)号：EP4219720A2

公开(公告)日：2023-08-02

申请号：EP22208929.4

申请日：2019-06-12

申请人： Caribou Biosciences, Inc.

发明人： CAMERON, Peter, Sean ,  GRADIA, Scott, David ,  KLOMPE, Sanne, Eveline ,  STERNBERG, Samuel, Henry ,  THOMPSON, Matthew, Scott

IPC分类号： C12N15/62 ,  C12N9/16 ,  A61K48/00

摘要： The present disclosure provides engineered Class 1 Type I CRISPR-Cas (Cascade) systems that comprise multi-protein effector complexes, nucleoprotein complexes comprising Type I CRISPR-Cas subunit proteins and nucleic acid guides, polynucleotides encoding Type I CRISPR-Cas subunit proteins, and guide polynucleotides. Also, disclosed are methods for making and using the engineered Class 1 Type I CRISPR-Cas systems of the present invention.

公开(公告)号：EP3620534A1

公开(公告)日：2020-03-11

申请号：EP19188624.1

申请日：2014-03-12

申请人： Caribou Biosciences, Inc.

发明人： MAY, Andrew, Paul ,  HAURWITZ, Rachel, E. ,  DOUDNA, Jennifer, A. ,  BERGER, James, M. ,  CARTER, Matthew, Merrill ,  DONOHOUE, Paul

IPC分类号： C12Q1/6818 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12N9/22 ,  C12N15/113 ,  C12N15/52 ,  C12N15/10

摘要： This disclosure provides for compositions and methods for the use of nucleic acid-targeting nucleic acids and complexes thereof. Genome engineering can refer to altering the genome by deleting, inserting, mutating, or substituting specific nucleic acid sequences. The altering can be gene or location specific. Genome engineering can use nucleases to cut a nucleic acid thereby generating a site for the alteration. Engineering of non-genomic nucleic acid is also contemplated.

公开(公告)号：EP3526324A1

公开(公告)日：2019-08-21

申请号：EP18720431.8

申请日：2018-03-27

申请人： Caribou Biosciences, Inc.

发明人： CARTER, Matthew Merrill ,  DONOHOUE, Paul Daniel

IPC分类号： C12N9/22 ,  C12N15/11

公开(公告)号：EP3420080B1

公开(公告)日：2019-08-21

申请号：EP17711029.3

申请日：2017-02-21

申请人： Caribou Biosciences, Inc.

发明人： CAPURSO, Daniel ,  MAY, Andrew P. ,  VAN OVERBEEK, Megan

IPC分类号： C12N15/10 ,  C12N15/11 ,  C12N15/90

公开(公告)号：EP3461894A1

公开(公告)日：2019-04-03

申请号：EP18200117.2

申请日：2016-08-05

申请人： Caribou Biosciences, Inc.

发明人： DONOHOUE, Paul, Daniel ,  MAY, Andrew, Paul

IPC分类号： C12N15/11 ,  C12N15/113

摘要： The present specification discloses engineered Type II CRISPR-Cas9-associated split-nexus polynucleotide (sn-casPNs) systems, including systems comprising three split- nexus polynucleotides (snl-casPN/sn2-casPN/sn3casPN) and systems comprising two split-nexus polynucleotides (snl-casPN/sn2-casPN). Together with a Cas9 protein, the sn-casPNs facilitate site-specific modifications, including cleavage and mutagenesis, of a target polynucleotide in vitro and in vivo. Furthermore, the engineered Type II CRISPR-Cas9-associated split-nexus polynucleotide systems comprising sn-casPNs are useful in methods of regulating expression of a target nucleic acid. Methods are described herein for the creation of a variety of engineered Type II CRISPR- Cas9-associated split-nexus polynucleotide systems comprising two or more sn-casPNs. Polynucleotide sequences, expression cassettes, vectors, compositions, and kits for carrying out a variety of methods are also described. Furthermore, the present specification provides genetically modified cells, compositions of modified cells, transgenic organisms, pharmaceutical compositions, as well as a variety of compositions and methods involving the engineered Type II CRISPR-Cas9-associated split-nexus polynucleotide systems.