摘要:
Genetically modified strains of C. tropicalis, which will not revert to wild-type activity at the POX 4 and/or POX 5 locus, are disclosed. The strains are β-oxidation blocked and have been transformed through homologous recombination with a construct which deletes a portion of the POX 4 and/or POX 5 gene. The modified strains may be used to increase yields of dicarboxylic acids produced in host cells of the strains. Methods for blocking the β-oxidation pathway in a C. tropicalis host cell are also provided.
摘要:
A process for detecting low levels of a predetermined quality trait in an inhomogeneously distributed particulate substrate (22) involving the steps of: (a) providing a particulate substrate (22) to be analyzed; (b) providing a spectrometer (12) with an electromagnetic detector capable of performing spectroscopic measurements with electromagnetic radiation; (c) providing a rotatable sample holder (14) having a transparent area through which electromagnetic radiation may pass; (d) providing a tumbling member (18) located within the rotatable sample holder (14) for tumbling the particulate substrate (22) contained therein; (e) introducing the particulate substrate (22) into the rotatable sample holder (14); (f) simultaneously rotating and tumbling the particulate substrate (22) contained within the rotatable sample holder (14); and (g) activating the spectrophotometer (12).
摘要:
Polyesters are described which are substantially the condensation products of at least one dicarboxylic acid, at least one volatile diol and at least one hydroxyl-functional component with functionality greater than 2 which controls ultimate viscosity. The products are suitable vehicles for the formulated thixotropic gels for centrifugal separation of fluids such as serum into high and low density components of, for example, separation of mixtures of cells on the basis of density.
摘要:
The invention is a method for improving the color and color stability of oleic acid by a two column distillation process. The oleic acid to be color improved is introduced into a first distillation zone (1) containing an enriching section (2) and a stripping section (3) providing a minor amount of a topping product and a major amount of underflow containing the oleic acid. Passing the oleic acid to a bottom portion of a second distillation (24) zone having an enriching section (25) to recover the improved oleic acid with improved color and color stability as a top product from the second distillation zone.
摘要:
Processes for purifying compounds are disclosed which include reacting (I) a mixture of at least one tocopherol compound or sterol compound, and an alcohol having from about 10 to 30 carbons with (ii) one or more boron-containing compounds to form a second mixture of orthoborate esters, wherein the mole ratio of tocopherol, sterol and alcohol to boron is at least about 2.5:1; heating the second mixture to form a residue containing the orthoborate esters; contacting the residue with a compound capable of solvolyzing the orthoborate esters to form a third mixture including the boron-containing compound(s), the alcohol and the tocopherol and/or sterol compound(s); and recovering the tocopherol and/or sterol compound(s). Another process for purifying such compounds includes an activated carbon treatment, which may alternatively follow the other process steps; formation of borate esters; heating to remove low boiling point components; formation of a residue comprising the borate esters; contacting the residue with a compound capable of solvolyzing the borate esters; and separation of the remaining components. Compositions including mixtures of alkyl/tocopherol/sterol orthoborate esters are also disclosed.
摘要:
A gene has been isolated which encodes cytochrome b5 (CYTb5) protein of the omega-hydroxylase complex of C. tropicalids 20336. Vectors including this gene, and transformed host cells are provided. Methods of increasing the production of a CYTb5 protein are also provided which involve transforming a host cell with a gene encoding this protein and culturing the cells. Methods of increasing the production of a dicarboxylic acid are also provided which involve increasing in the host cell the number of genes encoding this protein.
摘要:
A substantially water-free, water-washable, energy-curable composition includes an epoxy oligomer and/or urethane oligomer having at least two ethylenically unsaturated moieties, at least one alkoxylated polyol monomer having at least two ethylenically unsaturated moieties and capable of being copolymerized with the oligomer, and a sugar surfactant capable of rendering the uncured composition dispersible in water. Optionally, the composition can contain a photoinitiator. The composition is self-dispersible in water and is especially suitable for use as a coating material for a printing screen.