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公开(公告)号：EP3077419B1
公开(公告)日：2019-08-14
申请号：EP14868266.9
申请日：2014-12-04
IPC分类号： C07K14/575 , C07K14/605 , C12N15/62 , C12N15/13 , G01N33/50
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公开(公告)号：EP3077419A1
公开(公告)日：2016-10-12
申请号：EP14868266.9
申请日：2014-12-04
IPC分类号： C07K19/00 , C07K14/575 , C07K14/605 , C07K16/28 , C12N15/13 , C12N15/62 , C12N15/85 , C12N5/10 , C12Q1/68 , C40B40/02 , C40B50/06 , G01N33/567
CPC分类号： C07K16/2869 , A61K38/00 , C07K14/575 , C07K14/57563 , C07K14/605 , C07K2317/35 , C07K2317/51 , C07K2317/522 , C07K2317/526 , C07K2317/56 , C07K2317/565 , C07K2317/569 , C07K2317/64 , C07K2318/10 , C07K2319/75 , C12N2510/00 , C12Q1/6897 , C40B40/02 , G01N33/5008 , G01N2333/726 , G01N2500/10
摘要： Peptide-grafted antibodies having agonist activity against a Class B GPCR and their uses, and methods for identifying same. Mammalian cell lines for high-throughput screening for agonists of a Class B GPCR are also provided that comprise a stably integrated recombinant nucleic acid construct comprising a reporter gene under control of a cAMP responsive promoter, the reporter gene encoding a cell surface expressed protein, and a stably integrated recombinant nucleic acid construct comprising a sequence encoding a Class B GPCR under control of a constitutive promoter.
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公开(公告)号：EP3383893A1
公开(公告)日：2018-10-10
申请号：EP16869450.3
申请日：2016-12-02
IPC分类号： C07K14/725 , A61K38/17 , A61K47/66 , A61K47/68 , A61K51/08 , C07K19/00 , C12N5/10 , G01N33/567
CPC分类号： C07K16/2833 , A61K38/00 , C07K14/7051 , C07K19/00 , C07K2317/32 , C07K2317/569 , C07K2319/00 , C07K2319/03 , C07K2319/30 , C12N5/0636 , C12N2510/00 , G01N33/505 , G01N2333/7051
摘要： There is provided a single variable domain T-cell receptor (svd-TCR) comprising a first TCR variable domain, the first TCR variable domain specifically binding to an epitope, that is not a superantigen, in the absence of a second TCR variable domain. Also provided are compositions and cells comprising the svd-TCR as well as methods of identifying the svd-TCR.
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公开(公告)号：EP2271758B1
公开(公告)日：2018-09-12
申请号：EP09731924.8
申请日：2009-04-14
CPC分类号： C07K16/00 , C07K2317/56 , C12N15/113 , C12N2310/14
摘要： Compositions and methods are disclosed for generating immunoglobulin structural diversity in vitro, and in particular, for reducing biases in V region and J segment gene utilization, and for generating immunoglobulin V-D-J recombination events in a manner that does not require D-J recombination to precede V-DJ recombination. Selection of advantageous combinations of immunoglobulin gene elements, including introduction of artificial diversity (D) segment genes and optimization of recombination signal sequence (RSS) efficiency, are disclosed.
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公开(公告)号：EP2839009A4
公开(公告)日：2015-10-14
申请号：EP13760461
申请日：2013-03-14
发明人： GALLO MICHAEL , KANG JASPAL SINGH , PIGOTT CRAIG ROBIN , LIN ABBY
IPC分类号： C12N15/63 , C07K14/78 , C07K16/00 , C12N15/00 , C12N15/09 , C12N15/11 , C12N15/12 , C12N15/13 , C12N15/85 , C40B50/06
CPC分类号： C07K14/78 , C07K16/00 , C07K16/005 , C07K16/32 , C07K2317/14 , C07K2317/565 , C07K2318/00 , C07K2318/20 , C07K2319/03 , C07K2319/30 , C12N15/102
摘要： Methods of generating sequence diversity in a protein, such as a ligand-binding protein, are provided. The methods comprise targeted introduction of two or more recombination signal sequences (RSSs) into the protein coding sequence and introduction of the modified protein coding sequence into a recombination-competent host cell, specifically a recombination-competent host cell that is capable of expressing at least RAG-1 and RAG-2, thereby allowing for recombination of the protein coding sequence and expression of variant proteins. Also provided are polynucleotides comprising a nucleic acid sequence encoding a target protein, such as a ligand-binding protein, and comprising two or more RSSs, and compositions and host cells comprising same.
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6.发明公开GENERATING TARGETED SEQUENCE DIVERSITY IN PROTEINS 审中-公开埃泽尔恩EERER GEZIELTENFREQUENZDIVERSITÄTIN PROTEINEN
公开(公告)号：EP2839009A1
公开(公告)日：2015-02-25
申请号：EP13760461.7
申请日：2013-03-14
IPC分类号： C12N15/63 , C07K14/78 , C07K16/00 , C12N15/00 , C12N15/09 , C12N15/11 , C12N15/12 , C12N15/13 , C12N15/85 , C40B50/06
CPC分类号： C07K14/78 , C07K16/00 , C07K16/005 , C07K16/32 , C07K2317/14 , C07K2317/565 , C07K2318/00 , C07K2318/20 , C07K2319/03 , C07K2319/30 , C12N15/102
摘要： Methods of generating sequence diversity in a protein, such as a ligand-binding protein, are provided. The methods comprise targeted introduction of two or more recombination signal sequences (RSSs) into the protein coding sequence and introduction of the modified protein coding sequence into a recombination-competent host cell, specifically a recombination-competent host cell that is capable of expressing at least RAG-1 and RAG-2, thereby allowing for recombination of the protein coding sequence and expression of variant proteins. Also provided are polynucleotides comprising a nucleic acid sequence encoding a target protein, such as a ligand-binding protein, and comprising two or more RSSs, and compositions and host cells comprising same.
摘要翻译： 提供了在蛋白质如配体结合蛋白质中产生序列多样性的方法。 所述方法包括将两个或多个重组信号序列（RSS）有针对性地引入到蛋白质编码序列中，并将修饰的蛋白质编码序列导入可复性的宿主细胞，特别是能够至少表达重组的宿主细胞 RAG-1和RAG-2，从而允许蛋白质编码序列的重组和变体蛋白的表达。 还提供了包含编码靶蛋白的核酸序列的多核苷酸，例如配体结合蛋白，并且包含两个或更多个RSS，以及包含其的组合物和宿主细胞。
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公开(公告)号：EP2825653A1
公开(公告)日：2015-01-21
申请号：EP13761957.3
申请日：2013-03-14
IPC分类号： C12N15/63 , C07K14/575 , C07K14/605 , C07K16/00 , C07K19/00 , C12N15/09 , C12N15/11 , C12N15/13 , C12N15/62 , C12N15/85
CPC分类号： C12N15/625 , C07K14/4723 , C07K14/522 , C07K14/5421 , C07K14/57563 , C07K14/605 , C07K14/71 , C07K14/78 , C07K16/00 , C07K16/2839 , C07K16/2866 , C07K16/2869 , C07K16/32 , C07K2317/565 , C07K2318/10 , C07K2319/00 , C07K2319/01 , C07K2319/03 , C12N15/62 , C12N15/85 , C12N2800/30
摘要： Methods of generating fusion protein variants are provided that comprise introducing sequence diversity at the junction region or regions in the fusion and allows for the generation of variants having a desired activity. Examples include immunoglobulins comprising a domain or polypeptide inserted into, or replacing, a CDR. Also provided are polynucleotides encoding a fusion protein and comprising two or more RSSs, and compositions and host cells comprising same, as well as fusion proteins variants produced by the described methods.
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8.发明公开SEQUENCE DIVERSITY GENERATION IN IMMUNOGLOBULINS 审中-公开ERZEUGUNG VONSEQUENZDIVERSITÄTBEI IMMUNGLOBULINEN
公开(公告)号：EP2271758A4
公开(公告)日：2012-05-23
申请号：EP09731924
申请日：2009-04-14
IPC分类号： C12N15/13 , C07K16/18 , C12N15/113 , C12N15/63
CPC分类号： C07K16/00 , C07K2317/56 , C12N15/113 , C12N2310/14
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公开(公告)号：EP2825653A4
公开(公告)日：2016-01-20
申请号：EP13761957
申请日：2013-03-14
IPC分类号： C07K14/575 , C07K14/47 , C07K14/52 , C07K14/54 , C07K14/605 , C07K14/71 , C07K14/78 , C07K16/28 , C07K16/32 , C12N15/62 , C12N15/85
CPC分类号： C12N15/625 , C07K14/4723 , C07K14/522 , C07K14/5421 , C07K14/57563 , C07K14/605 , C07K14/71 , C07K14/78 , C07K16/00 , C07K16/2839 , C07K16/2866 , C07K16/2869 , C07K16/32 , C07K2317/565 , C07K2318/10 , C07K2319/00 , C07K2319/01 , C07K2319/03 , C12N15/62 , C12N15/85 , C12N2800/30
摘要： Methods of generating fusion protein variants are provided that comprise introducing sequence diversity at the junction region or regions in the fusion and allows for the generation of variants having a desired activity. Examples include immunoglobulins comprising a domain or polypeptide inserted into, or replacing, a CDR. Also provided are polynucleotides encoding a fusion protein and comprising two or more RSSs, and compositions and host cells comprising same, as well as fusion proteins variants produced by the described methods.
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公开(公告)号：EP2839009B1
公开(公告)日：2019-08-21
申请号：EP13760461.7
申请日：2013-03-14
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