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公开(公告)号：EP2825653A4
公开(公告)日：2016-01-20
申请号：EP13761957
申请日：2013-03-14
IPC分类号： C07K14/575 , C07K14/47 , C07K14/52 , C07K14/54 , C07K14/605 , C07K14/71 , C07K14/78 , C07K16/28 , C07K16/32 , C12N15/62 , C12N15/85
CPC分类号： C12N15/625 , C07K14/4723 , C07K14/522 , C07K14/5421 , C07K14/57563 , C07K14/605 , C07K14/71 , C07K14/78 , C07K16/00 , C07K16/2839 , C07K16/2866 , C07K16/2869 , C07K16/32 , C07K2317/565 , C07K2318/10 , C07K2319/00 , C07K2319/01 , C07K2319/03 , C12N15/62 , C12N15/85 , C12N2800/30
摘要： Methods of generating fusion protein variants are provided that comprise introducing sequence diversity at the junction region or regions in the fusion and allows for the generation of variants having a desired activity. Examples include immunoglobulins comprising a domain or polypeptide inserted into, or replacing, a CDR. Also provided are polynucleotides encoding a fusion protein and comprising two or more RSSs, and compositions and host cells comprising same, as well as fusion proteins variants produced by the described methods.
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公开(公告)号：EP2839009A4
公开(公告)日：2015-10-14
申请号：EP13760461
申请日：2013-03-14
发明人： GALLO MICHAEL , KANG JASPAL SINGH , PIGOTT CRAIG ROBIN , LIN ABBY
IPC分类号： C12N15/63 , C07K14/78 , C07K16/00 , C12N15/00 , C12N15/09 , C12N15/11 , C12N15/12 , C12N15/13 , C12N15/85 , C40B50/06
CPC分类号： C07K14/78 , C07K16/00 , C07K16/005 , C07K16/32 , C07K2317/14 , C07K2317/565 , C07K2318/00 , C07K2318/20 , C07K2319/03 , C07K2319/30 , C12N15/102
摘要： Methods of generating sequence diversity in a protein, such as a ligand-binding protein, are provided. The methods comprise targeted introduction of two or more recombination signal sequences (RSSs) into the protein coding sequence and introduction of the modified protein coding sequence into a recombination-competent host cell, specifically a recombination-competent host cell that is capable of expressing at least RAG-1 and RAG-2, thereby allowing for recombination of the protein coding sequence and expression of variant proteins. Also provided are polynucleotides comprising a nucleic acid sequence encoding a target protein, such as a ligand-binding protein, and comprising two or more RSSs, and compositions and host cells comprising same.
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3.发明公开SEQUENCE DIVERSITY GENERATION IN IMMUNOGLOBULINS 审中-公开ERZEUGUNG VONSEQUENZDIVERSITÄTBEI IMMUNGLOBULINEN
公开(公告)号：EP2271758A4
公开(公告)日：2012-05-23
申请号：EP09731924
申请日：2009-04-14
IPC分类号： C12N15/13 , C07K16/18 , C12N15/113 , C12N15/63
CPC分类号： C07K16/00 , C07K2317/56 , C12N15/113 , C12N2310/14
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4.发明公开G-PROTEIN COUPLED RECEPTOR AGONISTS AND METHODS 有权G-PROTEIN-GEKOPPELTE REZEPTORAGONISTEN UND VERFAHREN
公开(公告)号：EP3077419A4
公开(公告)日：2017-05-17
申请号：EP14868266
申请日：2014-12-04
IPC分类号： C07K19/00 , C07K14/575 , C07K14/605 , C07K16/28 , C12N5/10 , C12N15/13 , C12N15/62 , C12N15/85 , C12Q1/68 , C40B40/02 , C40B50/06 , G01N33/567
CPC分类号： C07K16/2869 , A61K38/00 , C07K14/575 , C07K14/57563 , C07K14/605 , C07K16/28 , C07K2317/51 , C07K2317/526 , C07K2317/56 , C07K2317/565 , C07K2318/10 , C07K2319/75 , C12N2510/00 , C12Q1/68 , C12Q1/6897 , C40B40/02 , C40B50/06 , G01N33/5008 , G01N33/567 , G01N2333/726 , G01N2500/10
摘要： Peptide-grafted antibodies having agonist activity against a Class B GPCR and their uses, and methods for identifying same. Mammalian cell lines for high-throughput screening for agonists of a Class B GPCR are also provided that comprise a stably integrated recombinant nucleic acid construct comprising a reporter gene under control of a cAMP responsive promoter, the reporter gene encoding a cell surface expressed protein, and a stably integrated recombinant nucleic acid construct comprising a sequence encoding a Class B GPCR under control of a constitutive promoter.
摘要翻译： 具有针对B类GPCR的激动剂活性的肽移植抗体及其用途，以及鉴定它们的方法。 还提供了用于B类GPCR激动剂的高通量筛选的哺乳动物细胞系，其包含稳定整合的重组核酸构建体，所述稳定整合的重组核酸构建体包含处于cAMP响应性启动子控制下的报道基因，编码细胞表面表达蛋白的报道基因和 包含编码在组成型启动子控制下的B类GPCR的序列的稳定整合的重组核酸构建体。
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