公开(公告)号：EP1947459A2

公开(公告)日：2008-07-23

申请号：EP08075097.9

申请日：2001-08-24

Applicant: NANOGEN, INC.

Inventor： Shi, Qinwei

IPC: G01N33/58 ,  G01N33/543 ,  G01N33/542 ,  G01N33/532 ,  G01N33/68 ,  G01N33/573 ,  C07K14/00

CPC classification number: G01N33/532 ,  G01N33/542 ,  G01N33/54306 ,  G01N33/573 ,  G01N33/581 ,  G01N33/6887

Abstract: The invention provides assay methods and kits that in general measure the level of a first analyte in a sample reduced by the level of a second analyte present in the same sample. In one embodiment, where levels of a first analyte from a first source is desirably determined and first analyte in the sample released from a second source is accompanied by proportional co-release of a second analyte, the assay identifies the level of first analyte released only from the first source. For analytes within bodily fluids, the assay can differentiate between elevated levels of analyte specific to the particular physiological or pathological state and elevated levels not specific to the particular state, providing single tests with diagnostic utility.

Abstract translation: 本发明提供了测定方法和试剂盒，其通常测量样品中第一分析物的水平降低了同一样品中存在的第二分析物的水平。 在一个实施方案中，期望确定来自第一来源的第一分析物的水平，并且从第二来源释放的样品中的第一分析物伴随着第二分析物的比例共释放，所述测定确定仅释放的第一分析物的水平 从第一个来源。 对于体液中的分析物，该测定可以区分特定于特定生理或病理状态的特异性分析物的升高水平和不特定于特定状态的升高水平，提供具有诊断效用的单个测试。

公开(公告)号：EP1379802A4

公开(公告)日：2005-11-02

申请号：EP02707838

申请日：2002-02-22

Applicant: BECTON DICKINSON CO ,  NANOGEN INC

Inventor： WINGER THOMAS ,  EVANS JOHN ,  HARVEY NOEL

IPC: B81B3/00 ,  F03G7/00 ,  F15C5/00 ,  F16K7/12 ,  F16K7/14 ,  F16K31/02 ,  F16K99/00

CPC classification number: F16K99/0001 ,  B01L3/502738 ,  F15C5/00 ,  F16K7/123 ,  F16K7/14 ,  F16K31/02 ,  F16K99/0015 ,  F16K99/0051 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0084

Abstract: A microactuator device (12) includes a base (20) with at least one electrode pad (24) and a permeation membrane (18). Permeation membrane (18) is typically a water-permeable membrane that is able to deform by applying an electric charge to the electrode pad (24). The actuator device (12) can be incorporated into valve assembly (10) to open and close the valve. The valve assembly can have a reciprocating valve member (146) operated by the deforming of the water-permeable member. Alternatively, the valve assembly can have an opening (46) positioned to cooperate with the water-permeable membrane (18) so that the deformation of the membrane closes the opening.

公开(公告)号：EP1573040A3

公开(公告)日：2005-09-28

申请号：EP03771899.6

申请日：2003-07-25

Applicant: NANOGEN, INC.

Inventor： RADTKEY, Ray, R. ,  HELD, Lance, C. ,  WALLACE, Bruce ,  MENGE, Karen ,  CANTER, David

IPC: C12Q1/00

CPC classification number: C12Q1/6827 ,  C12Q1/6837 ,  Y10T436/143333 ,  C12Q2537/149 ,  C12Q2525/161 ,  C12Q2535/131 ,  C12Q2565/518 ,  C12Q2525/186

公开(公告)号：EP1240512A2

公开(公告)日：2002-09-18

申请号：EP00992315.2

申请日：2000-10-31

Applicant: Nanogen, Inc.

Inventor： HAVENS, John, R. ,  WINGER, Theodore, M. ,  KROTZ, Jain ,  SMOLKO, Dan ,  ONOFREY, Thomas, J.

IPC: G01N33/48 ,  C12Q1/68

CPC classification number: G01N27/40 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B40/06 ,  C40B40/10 ,  G01N33/5438

Abstract: Electronically addressable microchips having covalently bound permeation layers and methods of making such covalently bonded permeation layers to microchips are provided. The covalent bonding is derived from combining the use of electrodes with silane derivatives. Such chemistry provides the ability to apply an electronic bias to the electrodes of the microchip while preventing permeation layer delaminating from the electrode surface.

公开(公告)号：EP1204853A1

公开(公告)日：2002-05-15

申请号：EP00951038.9

申请日：2000-06-09

Applicant: NANOGEN, INC.

Inventor： HAVENS, John, R. ,  KRIHAK, Michael, K. ,  GREEF, Charles, H. ,  RAYMOND, Daniel, E. ,  HELLER, Michael, J.

IPC: G01N15/00

CPC classification number: B01L3/502761 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00689 ,  B01J2219/00713 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01J2219/00853 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/508 ,  B01L3/5085 ,  B01L2200/0647 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/069 ,  B01L2400/0415 ,  B01L2400/0421 ,  B82Y5/00 ,  B82Y10/00 ,  C07B2200/11 ,  C07H21/00 ,  C07K1/04 ,  C07K1/045 ,  C07K1/047 ,  C07K1/24 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B60/14 ,  G11C13/0014 ,  G11C13/0019 ,  G11C13/04 ,  G11C19/00 ,  H01L24/95 ,  H01L25/50 ,  H01L51/0595 ,  H01L2924/10253 ,  H01L2924/14 ,  C12Q2565/515 ,  C12Q2565/607 ,  H01L2924/00

Abstract: The present invention pertains to a method of, and a device created by, depositing an inorganic permeation layer on a micro-electronic device for molecular biological reactions. The permeation layer is preferably sol-gel. The sol-gel permeation layer can be created with pre-defined porosity, pore size distribution, pore morphology, and surface area. The sol-gel permeation layer may also function as the attachment layer of the micro-electric device.

公开(公告)号：EP1120156A3

公开(公告)日：2001-10-24

申请号：EP01106840.0

申请日：1994-10-26

Applicant: NANOGEN, INC.

Inventor： Heller, Michael J. ,  Tu, Eugene

IPC: G01N33/543 ,  C12Q1/68 ,  B01J19/00

CPC classification number: B01L3/5085 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00689 ,  B01J2219/00713 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01L3/502707 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2400/0421 ,  B82Y5/00 ,  B82Y10/00 ,  C07B2200/11 ,  C07H21/00 ,  C07K1/04 ,  C07K1/045 ,  C07K1/047 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B60/14 ,  G01N27/3276 ,  G11C13/0014 ,  G11C13/0019 ,  G11C19/00 ,  H01L24/95 ,  H01L25/50 ,  H01L29/6656 ,  H01L29/6659 ,  H01L29/66628 ,  H01L29/7834 ,  H01L2224/45124 ,  H01L2224/45144 ,  H01L2224/95085 ,  H01L2224/95145 ,  H01L2924/00014 ,  H01L2924/09701 ,  H01L2924/10253 ,  H01L2924/12033 ,  H01L2924/12043 ,  H01L2924/15788 ,  Y10S435/808 ,  Y10S435/814 ,  Y10S436/805 ,  Y10T436/25125 ,  C12Q2565/515 ,  C12Q2565/607 ,  H01L2924/00 ,  H01L2224/48

Abstract: The invention provides for a method for electronically controlled hybridization of DNA from a solution containing specific binding and non-specific binding DNA sequences to a binding location.

公开(公告)号：EP1019711A4

公开(公告)日：2001-06-13

申请号：EP97938378

申请日：1997-08-18

Applicant: NANOGEN INC

Inventor： SOSNOWSKI RONALD GEORGE ,  BUTLER WILLIAM FRANK ,  TU EUGENE ,  NERENBERG MICHAEL IRVING ,  HELLER MICHAEL JAMES

IPC: G01N33/53 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N27/447 ,  G01N33/566 ,  G01N27/26 ,  C12N15/00

CPC classification number: H01L29/6656 ,  C12Q1/68 ,  C12Q1/6832

Abstract: The following inventions relate to discoveries concerning the various parameters, electrolytes (buffers), and other conditions which improve or optimize the speed of DNA transport, the efficiency of DNA hybridization reactions, and the overall hybridization specificity in microelectronic chips and devices. In particular, this invention relates to the discovery that low conductance zwitterionic buffer solutions, especially those containing the amino acid Histidine prepared at concentrations of ∩50 mM and at or near the pI (isoelectric point ∩pH 7.47), provide optimal conditions for both rapid electrophoretic DNA transport and efficient hybridization reactions. Hybridization efficiencies of at least a factor of 10 relative to the next best known buffer, Cysteine, are achieved. Test data demonstrate an approximately 50,000 fold increase in hybridization efficiency compared to Cysteine.

公开(公告)号：EP1088101A1

公开(公告)日：2001-04-04

申请号：EP00919981.1

申请日：2000-03-28

Applicant: NANOGEN, INC.

Inventor： GILES, Patrick, N. ,  DILLON, Patrick, J. ,  WU, David, J. ,  FOSTER, Charles B. ,  CHANOCK, Stephen J.

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/6825 ,  C12Q1/6837

Abstract: A rapid assay for single nucleotide polymorphism (SNP) detection that utilizes electronic circuitry on silicon microchips is disclosed. The method provides accurate discrimination of amplified DNA samples following electronic assisted transport, concentration, and attachment of DNA to selected electrodes (test sites). The test sites make up organized arrays of samples that are distinguished by using internal controls of dual labeled reporters comprising wild-type and mismatched sequences to validate the SNP genotype. This method has been used to discriminate the complex quadra-allelic SNP of mannose binding protein.

公开(公告)号：EP1053055A1

公开(公告)日：2000-11-22

申请号：EP99903423.4

申请日：1999-01-26

Applicant: NANOGEN, INC.

Inventor： CHENG, Jing ,  SHELDON, Edward, L., III ,  WU, Lei ,  O'CONNELL, James, P.

IPC: B01J19/00 ,  B01L3/00 ,  B03C5/02 ,  G01N33/53 ,  C12Q1/68 ,  C12N13/00

CPC classification number: B01L3/5027 ,  B01D57/02 ,  B01J19/0093 ,  B01J2219/00853 ,  B01J2219/00914 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0496 ,  B01L2400/0644 ,  B03C5/026 ,  B03C5/028 ,  C12N13/00 ,  G01N27/44743 ,  H01L29/6656

Abstract: The present invention comprises devices and methods for performing channel-less separation of cell particles by dielectrophoresis, DC high voltage-pulsed electronic lysis of separated cells, separation of desired components from crude mixtures such as cell lysates, and/or enzymatic reaction of such lysates, all of which can be conducted on a single bioelectronic chip. A preferred embodiment of the present invention comprises a cartridge (10) including a microfabricated silicon chip (12) on a printed circuit board (14) and a flow cell (16) mounted to the chip (12) to form a flow chamber. The cartridge (10) also includes output pins (22) for electronically connecting the cartridge (10) to an electronic controller. The chip (12) includes a plurality of circular microelectrodes (24) which are preferably coated with a protective permeation layer which prevents direct contact between any electrode and a sample introduced into the flow chamber. The permeation layer also helps to reduce cell adhesion at field minima, and enables immobilization of specific antibodies for specific cell capture. Specific cells from various cell mixtures were separated, lysed, and enzymatically digested on the chip.

公开(公告)号：EP2215450A4

公开(公告)日：2011-06-29

申请号：EP07751240

申请日：2007-02-21

Applicant: NANOGEN INC

Inventor： BOOKER DAVID DICKSON ,  LIDGARD GRAHAM PETER ,  EGAN RICHARD LASWELL ,  JOHNSON CHRISTOPHER JOHANN

IPC: G01N33/543 ,  C07H21/02 ,  C12Q1/00 ,  C12Q1/68 ,  C12Q1/70 ,  G01N15/06 ,  G01N33/00 ,  G01N33/53 ,  G01N33/58

CPC classification number: G01N33/56983 ,  B01L3/5023 ,  B01L3/5029 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0832 ,  B01L2300/087 ,  B01L2400/0478 ,  B01L2400/0683 ,  C12Q1/701 ,  G01N33/558 ,  G01N2333/11 ,  G01N2469/10 ,  Y10S435/81 ,  Y10S436/808 ,  Y10S436/81