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15 条记录 (耗时 0.013 秒)

    DEGRADABLE NUCLEIC ACID PROBES AND NUCLEIC ACID DETECTION METHODS
    1.
    发明公开
    DEGRADABLE NUCLEIC ACID PROBES AND NUCLEIC ACID DETECTION METHODS 审中-公开
    可生物降解NUKLEISÄURESONDEN及检测方法FORNUKLEISÄUREN

    公开(公告)号：EP1280938A2

    公开(公告)日：2003-02-05

    申请号：EP01932581.0

    申请日：2001-04-18

    申请人： NAXCOR

    发明人： VANATTA, Reuel ,  ALBAGLI, David ,  WOOD, Michael, L. ,  CHENG, Peter, C.

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6816 ,  C12Q2523/313 ,  C12Q2523/101

    摘要： Nucleic acid probes that are susceptible to chemical or enzymatic degradation are described herein. In addition, assays and methods using such probes in the detection of target nucleic acid sequences are disclosed. The target-specific hybridization region or target-complementary region of the degradable probes can be separated via a degradation process from the detectable region. The remaining portion of the degradable probes can be easily detected. The use of the degradable probes described herein improves the signal-to-noise ratio by reducing background specific or non-specific signal generation in assays and methods of nucleic acid detection.

    COUMARIN DERIVATIVES FOR USE AS NUCLEOTIDE CROSSLINKING REAGENTS
    2.
    发明授权
    COUMARIN DERIVATIVES FOR USE AS NUCLEOTIDE CROSSLINKING REAGENTS 失效
    用作核苷交联试剂的香豆素衍生物

    公开(公告)号：EP0466773B1

    公开(公告)日：1996-06-12

    申请号：EP90905935.4

    申请日：1990-03-23

    申请人： NAXCOR

    发明人： SABA, Don ,  GLASS, Richard, S. ,  YABUSAKI, Kenichi, K.

    IPC分类号： C07H15/26 ,  C07H21/00

    CPC分类号： C07H21/00 ,  C07H15/26

    摘要： A photoactivatable nucleoside analogue is disclosed, comprising a coumarin moiety linked through its phenyl ring to the 1-position of a ribose or deoxyribose sugar moiety. The resulting nucleoside analogue is typically used as a photocrosslinking group when inserted into a polynucleotide as a replacement for one or more of the complementary nucleoside bases present in a probe used in a hybridization assay.

    摘要翻译： 公开了可光活化的核苷类似物，其包含通过其苯环连接至核糖或脱氧核糖糖部分的1-位的香豆素部分。 当插入到多核苷酸中时，所得到的核苷类似物通常用作光交联基团，作为用于杂交测定的探针中存在的一个或多个互补核苷碱基的替代物。

    DOUBLE-STRANDED CONFORMATIONAL POLYMORPHISM ANALYSIS
    4.
    发明公开
    DOUBLE-STRANDED CONFORMATIONAL POLYMORPHISM ANALYSIS 失效
    多态性的AT双构象分析

    公开(公告)号：EP0863998A4

    公开(公告)日：2002-09-25

    申请号：EP96937086

    申请日：1996-11-01

    申请人： NAXCOR

    发明人： WOOD MICHAEL ,  VAN ATTA REUEL ,  ALBAGLI DAVID

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6827 ,  Y10S435/81 ,  C12Q2565/131 ,  C12Q2523/101 ,  C12Q2523/313 ,  C12Q2565/125

    摘要： Double-stranded conformational polymorphism analysis is performed by combining a probe comprising a cross-linking agent and optionally a label with a sample having a target sequence, which may be complementary or have one or a few mismatches with respect to the probe sequence. After sufficient time for hybridization under mild or lesser stringency conditions, hybridized pairs are irradiated to induce cross-link formation by the cross-linking agent. The sample is then analyzed by denaturing gel electrophoresis where the rate of migration depends upon the degree of complementarity between the probe and the target. For corroboration, in a second experiment, the probe may be combined with the sample under high stringency conditions, where it is found that the formation of cross-linked probe/target is substantially lower for pairs having mismatches than for fully matched pairs. After cross-linking, the sample may be separated by gel electrophoresis, and the amount of cross-linked nucleic acid determined.

    ASSAYS USING CROSSLINKABLE IMMOBILIZED NUCLEIC ACIDS
    5.
    发明公开
    ASSAYS USING CROSSLINKABLE IMMOBILIZED NUCLEIC ACIDS 审中-公开
    测试程序使用固定NUCLEIC联

    公开(公告)号：EP1105539A2

    公开(公告)日：2001-06-13

    申请号：EP99945105.7

    申请日：1999-08-23

    申请人： NAXCOR

    发明人： HUAN, Bingfang ,  ALBAGLI, David ,  WOOD, Michael, L. ,  VAN ATTA, Reuel, B. ,  CHENG, Peter, C.

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q1/6832 ,  C12Q1/6837 ,  C12Q1/6841 ,  C12Q2537/143 ,  C12Q2565/518 ,  C12Q2545/114 ,  C12Q2523/101

    摘要： Improved methods for in situ hybridization assays of cellular and subcellular systems and tissue sections, and immobilization-based assay techniques such as Northern blotting, Southern blotting, dot blots, and the like, and assay techniques wherein the probes are bound to substrates are disclosed. The subject invention employs crosslinker containing hybridization probes capable of forming covalent bonds between the probe and the target nucleic acid. Upon activation, the crosslinker will, if the probe has hybridized with its essentially complementary target, form covalent bonds with the complementary strand to covalently crosslink the probe to the target. Subsequently, stringent wash conditions may be employed to reduce background signals due to non-specific absorption or probes or targets, while retaining all crosslinked probe/target hybrids. Also disclosed are diagnostic kits for use in clininical and diagnostic laboratories.

    NUCLEIC ACID SEQUENCE DETECTION EMPLOYING AMPLIFICATION PROBES
    7.
    发明公开
    NUCLEIC ACID SEQUENCE DETECTION EMPLOYING AMPLIFICATION PROBES 失效
    NUCLEIC检测中放大探针

    公开(公告)号：EP0796346A4

    公开(公告)日：2004-06-23

    申请号：EP95944246

    申请日：1995-12-22

    申请人： NAXCOR

    发明人： ALBAGLI DAVID ,  VANATTA REUEL ,  WOOD MICHAEL

    IPC分类号： G01N33/58 ,  B01L7/00 ,  C07H21/04 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  C07H21/02 ,  G01N15/06

    CPC分类号： C12Q1/6816 ,  B01L7/52 ,  B01L2300/1883 ,  Y10S435/81 ,  C12Q2525/301 ,  C12Q2523/101 ,  C12Q2537/119 ,  C12Q2523/313

    摘要： Methods and compositions are provided for detecting nucleic acid sequences. In particular, pairs of probes are employed, where the pair defines a substantially contiguous sequence on a target nucleic acid. Each of the pairs has a side chain which forms a stem of the two side chains which non-covalently binds and is capable of forming a cross-link upon activation, when the probes and sample nucleic acid are base paired. Cross-linking of the stems when unbound to complementary DNA is inhibited. Each of the nucleic acids is initially present as single stranded nucleic acid to allow for base pairing, so that the probes bind to homologous target nucleic acid. The assay mixture is activated to provide cross-linking, the double stranded nucleic acid melted, and the process of base pairing, activation and melting repeated, a sufficient number of cycles, to provide a detectable amount of cross-linked probes. To inhibit background cross-linking, the side chains may provide for duplex formation, where a portion of the side chain binds to a different portion of the side chain or the portion of the probe homologous to the target. Also provided are kits comprising reagents, as well as automatic devices, for carrying out the subject method.