METHOD OF OBTAINING INDOLOCARBAZOLES USING BIOSYNTHETIC REBECCAMYCIN GENES
    4.
    发明公开
    METHOD OF OBTAINING INDOLOCARBAZOLES USING BIOSYNTHETIC REBECCAMYCIN GENES 有权
    VERFAHREN ZUR GEWINNUNG VON INDOLOCARBAZOLEN MIT HILFE VON REBECCAMYCIN-BIOSYNTHESEGENEN

    公开(公告)号:EP1443113A1

    公开(公告)日:2004-08-04

    申请号:EP02801342.3

    申请日:2002-10-17

    IPC分类号: C12N15/52 C12P19/28 C07H19/04

    CPC分类号: C12P19/28 C07H19/04 C12N15/52

    摘要: Method for production of indolocarbazoles by using rebeccamycin biosynthetic genes. The invention is based on utilization of rebeccamycin biosynthetic genes from Saccharothrix aerocolonigenes for production of indolocarbazoles in related microorganisms ( Streptomyces spp .) . Method includes the isolation of a DNA fragment from Saccharothrix aerocolonigenes ATCC39243 containing the rebeccamycin biosynthesis gene cluster and the expression of these genes in Streptomyces albus , achieving production of rebeccamycin and derivatives.
    To be applied in the pharmaceutical field.

    摘要翻译: 使用雷贝霉素生物合成基因生产吲哚卡巴唑的方法。 本发明是基于利用来自产气荚膜梭菌的雷贝霉素生物合成基因来生产相关微生物(链霉菌)中的吲哚卡巴唑。 方法包括分离含有重新卡非霉素生物合成基因簇的重组核酸菌(Saccharothrix aerocolonigenes)ATCC39243的DNA片段和这些基因在链霉菌(Streptomyces albus)中的表达,实现了雷贝卡霉素和衍生物的生产。 适用于制药领域。

    Vaccine against rabbit hemorrhagic disease virus
    5.
    发明公开
    Vaccine against rabbit hemorrhagic disease virus 失效
    Impfstoff gegenHämorrhagie-Vi​​rus aus Kaninchen

    公开(公告)号:EP0704529A2

    公开(公告)日:1996-04-03

    申请号:EP95500099.7

    申请日:1995-07-06

    摘要: Production of the structural antigen (VP60) of the virus of the hemorrhagic disease of the rabbits, RHDV, and its use as a vaccine against said disease. The production of the antigen and the method of vaccination comprise the following stages: a) isolation of the gene that code the structural protein (VP60) of the RHDV; b) cloning of said gene in the baculovirus transfer vector pAcYM1; c) obtention and isolation of the recombinant baculovirus AcVP60; d) infection of Sf9 insect cells with the recombinant baculovirus AcVP60; e) obtention and isolation of the protein VP60 produced by the recombinant baculovirus AcVP60; f) inoculation of the rabbits with the antigen.

    摘要翻译: 产生兔子出血性疾病病毒的结构抗原(VP60),RHDV,以及其作为针对所述疾病的疫苗的用途。 抗原的生成和接种方法包括以下阶段:a)分离编码RHDV的结构蛋白(VP60)的基因; b)在杆状病毒转移载体pAcYM1中克隆所述基因; c)获得并分离重组杆状病毒AcVP60; d)用重组杆状病毒AcVP60感染Sf9昆虫细胞; e)获得并分离由重组杆状病毒AcVP60产生的蛋白质VP60; f)用抗原接种兔子。

    ABSOLUTE METHOD FOR THE QUANTIFICATION OF ORGANIC COMPOUNDS
    9.
    发明公开
    ABSOLUTE METHOD FOR THE QUANTIFICATION OF ORGANIC COMPOUNDS 审中-公开
    绝对VERFAHREN ZUR QUANTIFIZIERUNG ORGANISCHER VERBINDUNGEN

    公开(公告)号:EP1939617A1

    公开(公告)日:2008-07-02

    申请号:EP06807961.5

    申请日:2006-10-11

    IPC分类号: G01N30/72 G01N31/12

    摘要: The invention relates to the quantification of organic compounds originating from a chromatographic system that does not involve the use of patterns, comprising the continuous conversion of the carbon of said compounds into carbon dioxide (CO2) involving a chemical reaction and the mixing of same, before or after separation, with a known, constant flow of a carbon compound, preferably CO2, which is enriched with carbon isotope-13 ( 13 C). The mixture is introduced into a mass spectrometer in which the isotopic ratio 12 C/ 13 C is continuously measured either in the carbon or in any characteristic ion originating from same. Said isotopic ratio is converted into a carbon mass flow measurement using in-line isotopic dilution.

    摘要翻译: 本发明涉及来自不涉及图案使用的色谱系统的有机化合物的定量,其包括将所述化合物的碳连续转化为涉及化学反应的二氧化碳(CO 2),并将其混合,然后 或分离后,用已知的恒定流量的碳化合物,优选富含碳同位素-13(13C)的CO 2。 将混合物引入质谱仪中,其中在碳或任何来自其的特征离子中连续测量同位素比12 C / 13 C。 使用在线同位素稀释将所述同位素比转化为碳质量流量测量。