公开(公告)号：EP3377657B1

公开(公告)日：2020-11-11

申请号：EP16867305.1

申请日：2016-11-18

申请人： 10X Genomics, Inc.

发明人： RYVKIN, Paul ,  UNDERWOOD, Jason ,  SCHNALL-LEVIN, Michael ,  MIKKELSEN, Tarjei Sigurd ,  HINDSON, Benjamin

IPC分类号： C12Q1/6806 ,  C12P19/34 ,  C12P19/30 ,  C40B40/06 ,  C12N15/10

公开(公告)号：EP3625715A1

公开(公告)日：2020-03-25

申请号：EP18802746.0

申请日：2018-05-18

申请人： 10X Genomics, Inc.

发明人： WONG, Alexander, Y. ,  MELLEN, Jeffrey ,  WU, Kevin ,  RYVKIN, Paul

IPC分类号： G06F19/26 ,  G06F19/10 ,  G06F19/24

公开(公告)号：EP3978622A1

公开(公告)日：2022-04-06

申请号：EP21194770.0

申请日：2017-12-22

申请人： 10X Genomics, Inc.

发明人： BELGRADER, Phillip ,  BENT, Zachary ,  GOPALAN, Vijay Kumar Sreenivasa ,  HARADA, Josephine ,  HINDSON, Christopher ,  LENJI, Mohammad Rahimi ,  MCDERMOTT, Geoffrey ,  MEER, Elliott ,  MIKKELSEN, Tarjei Sigurd ,  O'KEEFFE, Christopher Joachim ,  PFEIFFER, Katherine ,  PRICE, Andrew D. ,  RYVKIN, Paul ,  SAXONOV, Serge ,  STUELPNAGEL, John R. ,  TERRY, Jessica Michele

IPC分类号： C12Q1/6804 ,  C12N15/10 ,  C12Q1/6806

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for tagging analytes for characterization.

公开(公告)号：EP4043582A1

公开(公告)日：2022-08-17

申请号：EP22158749.6

申请日：2017-12-22

申请人： 10X Genomics, Inc.

发明人： BELGRADER, Phillip ,  BENT, Zachary ,  GOPALAN, Vijay Kumar Sreenivasa ,  HARADA, Josephine ,  HINDSON, Christopher ,  LENJI, Mohammad Rahimi ,  MCDERMOTT, Geoffrey ,  MEER, Elliott ,  MIKKELSEN, Tarjei Sigurd ,  O'KEEFFE, Christopher Joachim ,  PFEIFFER, Katherine ,  PRICE, Andrew D ,  RYVKIN, Paul ,  SAXONOV, Serge ,  STUELPNAGEL, John R. ,  TERRY, Jessica Michele

IPC分类号： C12Q1/6804 ,  C12N15/10 ,  C12Q1/6806

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for tagging analytes for characterization.

公开(公告)号：EP3913067A1

公开(公告)日：2021-11-24

申请号：EP21175267.0

申请日：2017-12-22

申请人： 10X Genomics, Inc.

发明人： BELGRADER, Phillip ,  BENT, Zachary ,  GOPALAN, Vijay Kumar Sreenivasa ,  HARADA, Josephine ,  HINDSON, Christopher ,  LENJI, Mohammad Rahimi ,  MCDERMOTT, Geoffrey ,  MEER, Elliott ,  MIKKELSEN, Tarjei Sigurd ,  O'KEEFFE, Christopher Joachim ,  PFEIFFER, Katherine ,  PRICE, Andrew D. ,  RYVKIN, Paul ,  SAXONOV, Serge ,  STUELPNAGEL, John R. ,  TERRY, Jessica Michele

IPC分类号： C12Q1/6804 ,  C12N15/10 ,  C12Q1/6806

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for tagging analytes for characterization.

公开(公告)号：EP3625361A1

公开(公告)日：2020-03-25

申请号：EP18812579.3

申请日：2018-11-15

申请人： 10X Genomics, Inc.

发明人： BENT, Zachary ,  MEER, Elliott ,  RIORDAN, Daniel ,  RYVKIN, Paul ,  SRINIVAS, Niranjan ,  TERRY, Jessica ,  GAGNON, Alex

IPC分类号： C12Q1/6806

公开(公告)号：EP3559265A2

公开(公告)日：2019-10-30

申请号：EP17885003.8

申请日：2017-12-22

申请人： 10X Genomics, Inc.

发明人： BELGRADER, Phillip ,  BENT, Zachary ,  GOPALAN, Vijay Kumar Sreenivasa ,  HARADA, Josephine ,  HINDSON, Christopher ,  LENJI, Mohammad Rahimi ,  MCDERMOTT, Geoffrey ,  MEER, Elliott ,  MIKKELSEN, Tarjei Sigurd ,  O'KEEFFE, Christopher Joachim ,  PFEIFFER, Katherine ,  PRICE, Andrew D. ,  RYVKIN, Paul ,  SAXONOV, Serge ,  STUELPNAGEL, John R. ,  TERRY, Jessica Michele

IPC分类号： C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6886 ,  C12Q1/6883 ,  C12P19/34 ,  C12N15/10

公开(公告)号：EP3954782A1

公开(公告)日：2022-02-16

申请号：EP21174805.8

申请日：2018-11-15

申请人： 10X Genomics, Inc.

发明人： BENT, Zachary ,  MEER, Elliott ,  RIORDAN, Daniel ,  RYVKIN, Paul ,  SRINIVAS, Niranjan ,  TERRY, Jessica ,  GAGNON, Alex

IPC分类号： C12Q1/6806

摘要： The present disclosure provides methods of generating supports (e.g., beads) comprising barcode molecules coupled thereto. A barcode molecule coupled to a support may comprise a barcode sequence and a functional sequence. A barcode molecule may be generated using two or more ligation reactions in a combinatorial fashion. A support comprising two or more different barcode molecules may be useful for analyzing or processing one or more analytes such as nucleic acid molecules, proteins, and/or perturbation agents.

公开(公告)号：EP3896171A1

公开(公告)日：2021-10-20

申请号：EP21169849.3

申请日：2017-12-22

申请人： 10X Genomics, Inc.

发明人： BELGRADER, Phillip ,  BENT, Zachary ,  GOPALAN, Vijay Kumar Sreenivasa ,  HARADA, Josephine ,  HINDSON, Christopher ,  LENJI, Mohammad Rahimi ,  MCDERMOTT, Geoffrey ,  MEER, Elliott ,  MIKKELSEN, Tarjei Sigurd ,  O'KEEFFE, Christopher Joachim ,  PFEIFFER, Katherine ,  PRICE, Andrew D. ,  RYVKIN, Paul ,  SAXONOV, Serge ,  STUELPNAGEL, John R. ,  TERRY, Jessica Michele

IPC分类号： C12Q1/6804 ,  C12N15/10 ,  C12Q1/6806

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for tagging analytes for characterization.

公开(公告)号：EP3775271A1

公开(公告)日：2021-02-17

申请号：EP19721887.8

申请日：2019-04-01

申请人： 10X Genomics, Inc.

发明人： BENT, Zachary ,  RYVKIN, Paul ,  TERRY, Jessica Michele ,  MCDERMOTT, Geoffrey ,  FREY, Meghan

IPC分类号： C12Q1/6806 ,  C12Q1/6869