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公开(公告)号：EP3536780A1
公开(公告)日：2019-09-11
申请号：EP16920642.2
申请日：2016-11-04
申请人： Asymchem Laboratories (Tianjin) Co., Ltd. , Asymchem Life Science (Tianjin) Co., Ltd. , Tianjin Asymchem Pharmaceuticals Co., Ltd. , Asymchem Laboratories (Fuxin) Co., Ltd. , Jilin Asymchem Laboratories Co., Ltd.
发明人： HONG, Hao , JAMES, Gage , LU, Jiangping , ZHANG, Na , YU, Wenyan , LIU, Fang , LI, Yanjun , HUANG, Xin , GAO, Juan , ZHANG, Kejian , MA, Yulei , WEI, Junlu
摘要： Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises (a) a protein having the amino acid sequence as shown in SEQ ID NO: 2; (b) a protein having an amino acid sequence of SEQ HD NO: 2 with a mutation of one or more amino acids and having a proline hydroxylase activity; or(c) a protein retaining the mutation of one or more amino acids as in (b), and having the proline hydroxylase activity and having at least 78% homology with the amino acid sequence of the protein in (b). Protein having the amino acid sequence as shown in SEQ HD NO: 2 and mutants obtained by genetically engineering have higher catalytic specificity or significantly increased catalytic activity when compared to proline hydroxylases in prior art.
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公开(公告)号：EP3712262A1
公开(公告)日：2020-09-23
申请号：EP17932405.8
申请日：2017-11-15
发明人： HONG, Hao , JAMES, Gage , LU, Jiangping , XU, Xingfu , YU, Wenyan , HUANG, Xin , MA, Yulei , CHENG, Yibing
摘要： A transaminase mutant and use hereof, the amino acid sequence of the transaminase mutant is an amino acid sequence in which the amino acid sequence as represented by SEQ ID NO: 1 is mutated, the mutated amino acid position being one or more selected from among F89, K193, P243, V234, I262, Q280, V379, R416, A417 and C418. The enzymatic activity and/or stability of the transaminase mutant is improved.
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公开(公告)号：EP3974523A1
公开(公告)日：2022-03-30
申请号：EP21193067.2
申请日：2017-11-15
发明人： HONG, Hao , JAMES, Gage , LU, Jiangping , XU, Xingfu , YU, Wenyan , HUANG, Xin , MA, Yulei , CHENG, Yibing
摘要： A transaminase mutant and use hereof, the transaminase mutant having the SEQ ID NO: 1 with one or more amino acid mutations selected from the group consisting of: R416A/C/H/Q/T/S, R416A/C/H/Q/T/S+A417S, R416A/C/H/Q/T/S+C418A/Q/S, R416A/C/H/Q/T/S+ F89Y/W, R416A/C/H/Q/T/S+A417S+ F89Y/W, wherein "/" stands for "or. The enzymatic activity and/or stability of the transaminase mutant is improved.
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公开(公告)号：EP3733689A1
公开(公告)日：2020-11-04
申请号：EP18903278.2
申请日：2018-02-05
发明人： HONG, Hao , JAMES, Gage , LU, Jiangping , XU, Xingfu , CUI, Yuxia , ZHANG, Na , DONG, Xuewu , YU, Wenyan , HUANG, Xin , HAO, Mingmin , MA, Yulei , CHENG, Yibing , ZHAO, Jiadong
IPC分类号： C07K14/195 , C12N9/10 , C12N15/54 , C12N15/63 , C12P41/00
摘要： The invention provides a transaminase mutant and application thereof, wherein the amino acid sequence of the transaminase mutant is formed after mutation of the amino acid sequence as shown in SEQ ID NO: 1, and mutated amino acid sites comprise T7C+S47C sites. The transaminase mutant having the mutation sites can be further prepared into an immobilized enzyme through an immobilization technology, the immobilized enzyme has relatively high activity and high stability, can be recycled for multiple times, and is applicable to continuous flow reaction in a packed bed.
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