公开(公告)号：EP3536780A1

公开(公告)日：2019-09-11

申请号：EP16920642.2

申请日：2016-11-04

申请人： Asymchem Laboratories (Tianjin) Co., Ltd. ,  Asymchem Life Science (Tianjin) Co., Ltd. ,  Tianjin Asymchem Pharmaceuticals Co., Ltd. ,  Asymchem Laboratories (Fuxin) Co., Ltd. ,  Jilin Asymchem Laboratories Co., Ltd.

发明人： HONG, Hao ,  JAMES, Gage ,  LU, Jiangping ,  ZHANG, Na ,  YU, Wenyan ,  LIU, Fang ,  LI, Yanjun ,  HUANG, Xin ,  GAO, Juan ,  ZHANG, Kejian ,  MA, Yulei ,  WEI, Junlu

IPC分类号： C12N9/02 ,  C12N15/53 ,  C12N1/21 ,  C12P13/24 ,  C12R1/19

摘要： Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises (a) a protein having the amino acid sequence as shown in SEQ ID NO: 2; (b) a protein having an amino acid sequence of SEQ HD NO: 2 with a mutation of one or more amino acids and having a proline hydroxylase activity; or(c) a protein retaining the mutation of one or more amino acids as in (b), and having the proline hydroxylase activity and having at least 78% homology with the amino acid sequence of the protein in (b). Protein having the amino acid sequence as shown in SEQ HD NO: 2 and mutants obtained by genetically engineering have higher catalytic specificity or significantly increased catalytic activity when compared to proline hydroxylases in prior art.

公开(公告)号：EP3075847B1

公开(公告)日：2019-05-15

申请号：EP14865656.4

申请日：2014-10-31

申请人： Asymchem Laboratories (Tianjin) Co., Ltd ,  Asymchem Life Science (Tianjin) Co., Ltd ,  Tianjin Asymchem Pharmaceutical Co., Ltd ,  Asymchem Laboratories (Fuxin) Co., Ltd ,  Jilin Asymchem Laboratories Co., Ltd

发明人： HONG, Hao ,  GAO, Feng ,  LI, Yanjun ,  ZHANG, Yan ,  LI, Shaohe

IPC分类号： C12N9/10 ,  C12N15/54 ,  C12P17/12 ,  C12P13/00 ,  C12R1/66 ,  C12R1/01

公开(公告)号：EP3075856A1

公开(公告)日：2016-10-05

申请号：EP14865169.8

申请日：2014-10-29

申请人： Asymchem Laboratories (Tianjin) Co., Ltd ,  Asymchem Life Science (Tianjin) Co., Ltd ,  Tianjin Asymchem Pharmaceutical Co., Ltd ,  Asymchem Laboratories (Fuxin) Co., Ltd ,  Jilin Asymchem Laboratories Co., Ltd

发明人： HONG, Hao ,  GAO, Feng ,  LI, Yanjun ,  ZHANG, Yan ,  LI, Shaohe

IPC分类号： C12N15/54 ,  C12N15/31 ,  C12N15/11 ,  C12N9/10 ,  C12P41/00 ,  C07K14/195

CPC分类号： C12N9/1096 ,  C12P13/001 ,  C12P17/12 ,  C12P41/006 ,  C12Y206/01

摘要： An omega-transaminase of R-configuration is provided. The omega-transaminase of R-configuration has the amino acid sequence as shown in SEQ ID NO: 2, or has at least 80% identity to the amino acid sequence as shown in SEQ ID NO: 2, or has the amino acid sequence of proteins which have substituted, deleted or added one or more amino acids and have the activity of an omega-transaminase with a high stereoselectivity R-configuration catalytic activity; and does not have the amino acid sequence encoded by the nucleotide sequence as shown in SEQ ID NO: 4. The high stereoselectivity refers to the content of one of the stereoisomers being at least about 1.1 times that of the other. A use of the omega-transaminase of R-configuration is provided, which can be used for highly efficient synthesis of a chiral amine of R-configuration with a relatively high chiral purity, and is therefore suitable for industrial production of chiral amines.

摘要翻译： 提供R-构型的ω-转氨酶。 R构型的ω-转氨酶具有如SEQ ID NO：2所示的氨基酸序列，或与SEQ ID NO：2所示的氨基酸序列具有至少80％的同一性，或具有SEQ ID NO：2所示的氨基酸序列 具有取代，缺失或添加一个或多个氨基酸并且具有高立体选择性R构型催化活性的ω-转氨酶活性的蛋白质; 并且不具有由SEQ ID NO：4所示的核苷酸序列编码的氨基酸序列。高立体选择性是指一种立体异构体的含量为至少约1.1倍。 提供R-构型的ω-转氨酶的用途，其可用于高效合成手性纯度较高的R-构型手性胺，因此适用于手性胺的工业生产。

公开(公告)号：EP3075847A1

公开(公告)日：2016-10-05

申请号：EP14865656.4

申请日：2014-10-31

申请人： Asymchem Laboratories (Tianjin) Co., Ltd ,  Asymchem Life Science (Tianjin) Co., Ltd ,  Tianjin Asymchem Pharmaceutical Co., Ltd ,  Asymchem Laboratories (Fuxin) Co., Ltd ,  Jilin Asymchem Laboratories Co., Ltd

发明人： HONG, Hao ,  GAO, Feng ,  LI, Yanjun ,  ZHANG, Yan ,  LI, Shaohe

IPC分类号： C12N9/10 ,  C12N15/54 ,  C12P17/12 ,  C12P13/00 ,  C12R1/66 ,  C12R1/01

CPC分类号： C12P13/001 ,  C12N1/20 ,  C12N9/1096 ,  C12N15/70 ,  C12P17/12 ,  C12Y206/01

摘要： Atransaminase and a use thereof are provided. The transaminase has the amino acid sequences as shown in SEQ ID NO: 2 or 4, or has at least 80% identity to the amino acid sequences as shown in SEQ ID NO: 2 or 4, or has amino acid sequences which are obtained by the substitution, deletion or addition of one or more amino acids and have an the activity of an omega-transaminase with high stereoselective R-configuration catalytic activity, wherein the high stereoselective refers to the content of one of the stereoisomers being at least about 1.1 times that of the other. The transaminase can synthesize a chiral amine of R-configuration with a relatively high chiral purity, and is therefore suitable for the industrial use of the synthesis of chiral amines.

摘要翻译： 提供了脱氨酶及其用途。 转氨酶具有如SEQ ID NO：2或4所示的氨基酸序列，或者与SEQ ID NO：2或4所示的氨基酸序列具有至少80％的同一性，或具有通过以下步骤获得的氨基酸序列： 一个或多个氨基酸的取代，缺失或添加并具有高立体选择性R构型催化活性的ω-转氨酶的活性，其中高立体选择性指立体异构体之一的含量为至少约1.1倍 那另一个。 转氨酶可以合成具有较高手性纯度的R-构型的手性胺，因此适用于工业上使用手性胺的合成。