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公开(公告)号：EP3536780A1
公开(公告)日：2019-09-11
申请号：EP16920642.2
申请日：2016-11-04
申请人： Asymchem Laboratories (Tianjin) Co., Ltd. , Asymchem Life Science (Tianjin) Co., Ltd. , Tianjin Asymchem Pharmaceuticals Co., Ltd. , Asymchem Laboratories (Fuxin) Co., Ltd. , Jilin Asymchem Laboratories Co., Ltd.
发明人： HONG, Hao , JAMES, Gage , LU, Jiangping , ZHANG, Na , YU, Wenyan , LIU, Fang , LI, Yanjun , HUANG, Xin , GAO, Juan , ZHANG, Kejian , MA, Yulei , WEI, Junlu
摘要： Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises (a) a protein having the amino acid sequence as shown in SEQ ID NO: 2; (b) a protein having an amino acid sequence of SEQ HD NO: 2 with a mutation of one or more amino acids and having a proline hydroxylase activity; or(c) a protein retaining the mutation of one or more amino acids as in (b), and having the proline hydroxylase activity and having at least 78% homology with the amino acid sequence of the protein in (b). Protein having the amino acid sequence as shown in SEQ HD NO: 2 and mutants obtained by genetically engineering have higher catalytic specificity or significantly increased catalytic activity when compared to proline hydroxylases in prior art.
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公开(公告)号：EP3974523A1
公开(公告)日：2022-03-30
申请号：EP21193067.2
申请日：2017-11-15
发明人： HONG, Hao , JAMES, Gage , LU, Jiangping , XU, Xingfu , YU, Wenyan , HUANG, Xin , MA, Yulei , CHENG, Yibing
摘要： A transaminase mutant and use hereof, the transaminase mutant having the SEQ ID NO: 1 with one or more amino acid mutations selected from the group consisting of: R416A/C/H/Q/T/S, R416A/C/H/Q/T/S+A417S, R416A/C/H/Q/T/S+C418A/Q/S, R416A/C/H/Q/T/S+ F89Y/W, R416A/C/H/Q/T/S+A417S+ F89Y/W, wherein "/" stands for "or. The enzymatic activity and/or stability of the transaminase mutant is improved.
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公开(公告)号：EP4417690A1
公开(公告)日：2024-08-21
申请号：EP22947559.5
申请日：2022-07-29
发明人： HONG, Hao , JAMES, Gage , ZHANG, Na , JIAO, Xuecheng , MA, Yulei , MU, Huiyan , XUAN, Meijuan , ZHANG, Yanqing , ZHANG, Renkuan
摘要： The present disclosure provides a transaminase mutant and a method for preparing a chiral amine compound. Herein, the transaminase mutant includes: (a) a protein having an amino acid sequence as shown in SEQ ID NO: 1; or (b) a protein having an amino acid sequence as shown in SEQ ID NO: 2 or SEQ ID NO: 3; or (c) a protein that undergoes an amino acid mutation in at least one of the following sites of the amino acid sequence in (b): Y89, L380, N86, Y85, T91, P83, K90, S417, S424, F301, G164, T452 and the like, and has a transaminase function; or (d) a protein having more than 80% of the homology with the amino acid sequence defined in any one of (a), (b) or (c) and having a transaminase function.
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公开(公告)号：EP3712262A1
公开(公告)日：2020-09-23
申请号：EP17932405.8
申请日：2017-11-15
发明人： HONG, Hao , JAMES, Gage , LU, Jiangping , XU, Xingfu , YU, Wenyan , HUANG, Xin , MA, Yulei , CHENG, Yibing
摘要： A transaminase mutant and use hereof, the amino acid sequence of the transaminase mutant is an amino acid sequence in which the amino acid sequence as represented by SEQ ID NO: 1 is mutated, the mutated amino acid position being one or more selected from among F89, K193, P243, V234, I262, Q280, V379, R416, A417 and C418. The enzymatic activity and/or stability of the transaminase mutant is improved.
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公开(公告)号：EP4257679A1
公开(公告)日：2023-10-11
申请号：EP21918810.9
申请日：2021-04-25
发明人： HONG, Hao , JAMES, Gage , ZHANG, Na , JIAO, Xuecheng , MA, Yulei , CAO, Shan , CHENG, Yibing , SUN, Kaihua
摘要： Provided are a cytochrome P450 enzyme mutant and an application thereof. The enzyme activity and anti-Markov oxidation selectivity of P450 derived from a wild-type strain of Bacillus megaterium undergo protein modification by means of directed evolution, thus improving enzyme activity and selectivity, and developing a series of P450 enzyme mutants that may be used for industrial production.
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公开(公告)号：EP3957724A1
公开(公告)日：2022-02-23
申请号：EP19925142.2
申请日：2019-04-17
发明人： HONG, Hao , JAMES, Gage , LU, Jiangping , ZHANG, Na , MA, Yulei , CHENG, Yibing , MU, Huiyan
摘要： Disclosed are a monooxygenase mutant and use thereof. An amino acid sequence of a monooxygenase mutant is an amino acid sequence obtained by mutating an amino acid sequence as shown in SEQ ID NO: 1. Mutation includes at least one of the following mutation sites: site 49, site 60, site 61, site 144, site 145, site 146, site 147, site 167, site 169, site 189, site 246, site 247, site 280, site 284, site 285, site 286, site 287, site 328, site 330, site 332, site 382, site 427, site 428, site 429, site 430, site 431, site 432, site 433, site 434, site 435, site 436, site 438, site 441, site 493, site 494, site 508, site 509, site 510, site 511, site 512, and site 513 sites and the like. The monooxygenase mutant has the advantage of greatly improved stereoselectivity, and the enzyme activity is also improved correspondingly.
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公开(公告)号：EP3733689A1
公开(公告)日：2020-11-04
申请号：EP18903278.2
申请日：2018-02-05
发明人： HONG, Hao , JAMES, Gage , LU, Jiangping , XU, Xingfu , CUI, Yuxia , ZHANG, Na , DONG, Xuewu , YU, Wenyan , HUANG, Xin , HAO, Mingmin , MA, Yulei , CHENG, Yibing , ZHAO, Jiadong
IPC分类号： C07K14/195 , C12N9/10 , C12N15/54 , C12N15/63 , C12P41/00
摘要： The invention provides a transaminase mutant and application thereof, wherein the amino acid sequence of the transaminase mutant is formed after mutation of the amino acid sequence as shown in SEQ ID NO: 1, and mutated amino acid sites comprise T7C+S47C sites. The transaminase mutant having the mutation sites can be further prepared into an immobilized enzyme through an immobilization technology, the immobilized enzyme has relatively high activity and high stability, can be recycled for multiple times, and is applicable to continuous flow reaction in a packed bed.
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公开(公告)号：EP3744836A1
公开(公告)日：2020-12-02
申请号：EP18901235.4
申请日：2018-01-22
发明人： HONG, Hao , JAMES, Gage , LU, Jiangping , XU, Xingfu , YU, Wenyan , ZHANG, Na , MA, Yulei , CHENG, Yibing , MU, Huiyan
摘要： A ketoreductase mutant and use thereof are provided. The amino acid sequence of the ketoreductase mutant is an amino acid sequence obtained by mutation of the amino acid sequence shown in SEQ ID NO: 1, wherein the mutation at least comprises one of the following mutation sites: position 6, position 94, position 96, position 117, position 144, position 156, position 193, position 205, position 224, position 176, position 85 and position 108; alternatively, the amino acid sequence of the ketoreductase mutant has a mutation site in a mutated amino acid sequence and an amino acid sequence having 80% or more homology with the mutated amino acid sequence. The ketoreductase mutants have high stability, especially the resistance to acetone and isopropanol such that when the mutants are used in the preparation of a chiral hydroxyl heterocyclic substance, the components of the reaction system are simplified, costs are lowered, and the prepared chiral alcohol has an ee value up to 99.8%.
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公开(公告)号：EP4144837A1
公开(公告)日：2023-03-08
申请号：EP20933800.3
申请日：2020-05-29
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , JIAO, Xuecheng , MA, Yulei , MOU, Huiyan , CAO, Shan
摘要： Provided are a transaminase mutant and an application thereof. Compared with an amino acid sequence shown in SEQ ID NO:1, an amino acid sequence of the transaminase mutant includes at least one of the following mutation sites: L166, K149, K146, A168, H73, F133, H82, E24, V194, T294, A295, G235 and F236. The mutant of the present invention has the improved catalytic activity for a transammonization reaction of ketone substrates, and is suitable for industrial production of chiral amines.
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公开(公告)号：EP4276188A1
公开(公告)日：2023-11-15
申请号：EP21924111.4
申请日：2021-07-06
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , JIAO, Xuecheng , MA, Yulei , MOU, Huiyan , WANG, Zujian , SUN, Kaihua , JIA, Ru , LIU, Fang , LIU, Wenjing
摘要： Provided is a method for synthesizing a chiral amine compound. A transaminase is used to transaminate a ketone substrate under the action of an amino donor, to obtain the chiral amine compound; and the conserved amino acid sequence region of the transaminase at least includes a region 1 (MAGLWCVN) and a region 2 (YNTFFKT). With the transaminase with the specific conserved amino acid sequence region to synthesize a large sterically hindered chiral amine, the enzyme catalytic reaction volume is small, the synthesizing route is short, the product yield is high, a high-cost noble metal is not required for catalysis under the synthesizing conditions, three wastes are reduced, and the production cost is saved.
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