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公开(公告)号：EP3536780A1
公开(公告)日：2019-09-11
申请号：EP16920642.2
申请日：2016-11-04
申请人： Asymchem Laboratories (Tianjin) Co., Ltd. , Asymchem Life Science (Tianjin) Co., Ltd. , Tianjin Asymchem Pharmaceuticals Co., Ltd. , Asymchem Laboratories (Fuxin) Co., Ltd. , Jilin Asymchem Laboratories Co., Ltd.
发明人： HONG, Hao , JAMES, Gage , LU, Jiangping , ZHANG, Na , YU, Wenyan , LIU, Fang , LI, Yanjun , HUANG, Xin , GAO, Juan , ZHANG, Kejian , MA, Yulei , WEI, Junlu
摘要： Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises (a) a protein having the amino acid sequence as shown in SEQ ID NO: 2; (b) a protein having an amino acid sequence of SEQ HD NO: 2 with a mutation of one or more amino acids and having a proline hydroxylase activity; or(c) a protein retaining the mutation of one or more amino acids as in (b), and having the proline hydroxylase activity and having at least 78% homology with the amino acid sequence of the protein in (b). Protein having the amino acid sequence as shown in SEQ HD NO: 2 and mutants obtained by genetically engineering have higher catalytic specificity or significantly increased catalytic activity when compared to proline hydroxylases in prior art.
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公开(公告)号：EP3141601A1
公开(公告)日：2017-03-15
申请号：EP14891354.4
申请日：2014-08-04
申请人： Asymchem Laboratories (Tianjin) Co., Ltd , Asymchem Life Science (Tianjin) Co., Ltd , Tianjin Asymchem Pharmaceutical Co. Ltd. , Asymchem Laboratories (Fuxin) Co., Ltd , Jilin Asymchem Laboratories Co., Ltd
发明人： HONG, Hao , GAGE, James , GAO, Feng , LIU, Lihui , YU, Wenyan , LIU, Fang , GUO, Lina , ZHANG, Na
CPC分类号： C12N9/0006 , C12N5/10 , C12N15/63 , C12P7/62 , C12Y101/01184
摘要： The invention provides a Diketoreductase (DKR) mutant, its nucleotide coding sequence, and an expression cassette, recombinant vector and host cell containing the sequence, as well as a method for application of the mutant to the preparation of 3R,5S-dicarbonyl compound. An ee value of the obtained 3R,5S-dicarbonyl compound is higher than 99%, and a de value is about 90%. The DKR mutant is a key pharmaceutical intermediate, and particularly provides an efficient catalyst for synthesis of a chiral dicarbonyl hexanoic acid chain of a statin drug.
摘要翻译： 本发明提供了二酮还原酶（DKR）突变体，其核苷酸编码序列，以及含有该序列的表达盒，重组载体和宿主细胞，以及将该突变体应用于制备3R，5S-二羰基化合物的方法。 所得3R，5S-二羰基化合物的ee值高于99％，de值约为90％。 DKR突变体是关键的药物中间体，并且特别提供了用于合成他汀类药物的手性二羰基己酸链的有效催化剂。
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公开(公告)号：EP3141601B1
公开(公告)日：2019-06-12
申请号：EP14891354.4
申请日：2014-08-04
申请人： Asymchem Laboratories (Tianjin) Co., Ltd , Asymchem Life Science (Tianjin) Co., Ltd , Tianjin Asymchem Pharmaceutical Co. Ltd. , Asymchem Laboratories (Fuxin) Co., Ltd , Jilin Asymchem Laboratories Co., Ltd
发明人： HONG, Hao , GAGE, James , GAO, Feng , LIU, Lihui , YU, Wenyan , LIU, Fang , GUO, Lina , ZHANG, Na
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公开(公告)号：EP4394035A1
公开(公告)日：2024-07-03
申请号：EP21956530.6
申请日：2021-10-13
发明人： HONG, Hao , JAMES, Gage , ZHANG, Na , JIAO, Xuecheng , LIU, Fang , JIA, Ru , LIU, Wenjing
摘要： Provided are a lipase mutant and an application thereof. Specifically, one or more mutations selected from A262H, A338V, V364I, A158P/V, and I159N are generated on the basis of an amino acid sequence as shown in SEQ ID NO: 1; and compared with a parental lipase, a change in the structure and function of a protein occurs in the lipase mutant, and the stereoselectivity is improved, such that a usage amount of an enzyme is decreased to a certain extent, the post-treatment difficulty is reduced, and the lipase mutant is suitable for industrial production.
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公开(公告)号：EP3964571A1
公开(公告)日：2022-03-09
申请号：EP19931297.6
申请日：2019-05-30
发明人： HONG, Hao , JAMES, Gage , ZHANG, Na , LIU, Fang , YAN, Junjie , LIU, Ye , WANG, Zujian
摘要： Provided are a transaminase mutant and a method for producing a chiral amine using the same. An amino acid sequence of the transaminase mutant is an amino acid sequence obtained by mutation occurred in an amino acid sequence as shown in SEQ ID NO: 1, and the mutation includes at least one of the following mutation sites: position 3, position 5, position 8, position 25, position 32, position 45, position 56, position 59, position 60, position 84, position 86, position 164, position 176, position 178, position 180, position 187, position 197, position 206, position 207, position 242, position 245, position 319 and position 324.
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公开(公告)号：EP4368732A2
公开(公告)日：2024-05-15
申请号：EP24165738.6
申请日：2020-07-08
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , LIU, Fang , WANG, Zujian , SHI, Wanming , YAN, Junjie , ZHANG, Mujiao
IPC分类号： C12R1/19
CPC分类号： C12N9/0004 , C12N15/70 , C12P7/02 , C12Y101/01 , C12R2001/1920210501
摘要： Provided is a ketoreductase mutant and applications thereof. Compared with an amino acid sequence shown in SEQ ID NO: 1, the amino acid sequence of the ketoreductase mutant includes at least one of the following mutation sites: L198P, S96W/I/L/V, M194L or L1526, herein "/" means "or." The ketoreductase mutant provides high selectivity and high activity with respect to a substrate ketone, and the application of the mutant in a catalytic reduction reaction of a ketone substrate may increase the production efficiency of a chiral alcohol compound corresponding thereto.
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公开(公告)号：EP4368733A2
公开(公告)日：2024-05-15
申请号：EP24165741.0
申请日：2020-07-08
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , LIU, Fang , WANG, Zujian , SHI, Wanming , YAN, Junjie , ZHANG, Mujiao
IPC分类号： C12R1/19
CPC分类号： C12N9/0004 , C12N15/70 , C12P7/02 , C12Y101/01 , C12R2001/1920210501
摘要： Provided is a ketoreductase mutant and applications thereof. Compared with an amino acid sequence shown in SEQ ID NO: 1, the amino acid sequence of the ketoreductase mutant includes at least one of the following mutation sites: L198P, S96W/I/L/V, M194L or L1526, herein "/" means "or." The ketoreductase mutant provides high selectivity and high activity with respect to a substrate ketone, and the application of the mutant in a catalytic reduction reaction of a ketone substrate may increase the production efficiency of a chiral alcohol compound corresponding thereto.
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公开(公告)号：EP4368733A3
公开(公告)日：2024-10-23
申请号：EP24165741.0
申请日：2020-07-08
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , LIU, Fang , WANG, Zujian , SHI, Wanming , YAN, Junjie , ZHANG, Mujiao
摘要： Provided is a ketoreductase mutant and applications thereof. Compared with an amino acid sequence shown in SEQ ID NO: 1, the amino acid sequence of the ketoreductase mutant includes at least one of the following mutation sites: L198P, S96W/I/L/V, M194L or L1526, herein "/" means "or." The ketoreductase mutant provides high selectivity and high activity with respect to a substrate ketone, and the application of the mutant in a catalytic reduction reaction of a ketone substrate may increase the production efficiency of a chiral alcohol compound corresponding thereto.
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公开(公告)号：EP4368732A3
公开(公告)日：2024-08-28
申请号：EP24165738.6
申请日：2020-07-08
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , LIU, Fang , WANG, Zujian , SHI, Wanming , YAN, Junjie , ZHANG, Mujiao
CPC分类号： C12N9/0004 , C12N15/70 , C12P7/02 , C12Y101/01 , C12R2001/1920210501
摘要： Provided is a ketoreductase mutant and applications thereof. Compared with an amino acid sequence shown in SEQ ID NO: 1, the amino acid sequence of the ketoreductase mutant includes at least one of the following mutation sites: L198P, S96W/I/L/V, M194L or L1526, herein "/" means "or." The ketoreductase mutant provides high selectivity and high activity with respect to a substrate ketone, and the application of the mutant in a catalytic reduction reaction of a ketone substrate may increase the production efficiency of a chiral alcohol compound corresponding thereto.
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公开(公告)号：EP4163368A1
公开(公告)日：2023-04-12
申请号：EP20939965.8
申请日：2020-07-08
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , LIU, Fang , WANG, Zujian , SHI, Wanming , YAN, Junjie , ZHANG, Mujiao
摘要： Provided is a ketoreductase mutant and applications thereof. Compared with an amino acid sequence shown in SEQ ID NO: 1, the amino acid sequence of the ketoreductase mutant includes at least one of the following mutation sites: L198P, S96W/I/L/V, M194L or L1526, herein "/" means "or." The ketoreductase mutant provides high selectivity and high activity with respect to a substrate ketone, and the application of the mutant in a catalytic reduction reaction of a ketone substrate may increase the production efficiency of a chiral alcohol compound corresponding thereto.
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