公开(公告)号：EP1147187B1

公开(公告)日：2012-06-27

申请号：EP00900645.3

申请日：2000-01-21

申请人： BIO MERIEUX

发明人： PARANHOS-BACCALA, Glaucia ,  MALLET, François ,  VOISSET, Cécile

IPC分类号： C12N15/11 ,  C07K14/15 ,  C12Q1/68

CPC分类号： C07K14/005 ,  C07K14/4713 ,  C12N2740/10022 ,  C12Q1/702

摘要： The invention concerns an endogenetic nucleic fragment, such as an isolated retrovirus, integrated in the human DNA genome, said fragment being characterised in that it comprises, or consists of, at least part of the gag gene of an endogenetic retrovirus associated with an autoimmune disease or pregnancy failure or pregnancy pathologies, said part at least coding, directly or indirectly, for an expression product, or the complementary of said fragment.

公开(公告)号：EP0920626B1

公开(公告)日：2003-02-26

申请号：EP98932247.4

申请日：1998-06-19

申请人： BIO MERIEUX

发明人： MALLET, François ,  DELAIR, Thierry ,  LADAVIERE, Catherine ,  NOVELLI-ROUSSEAU, Armelle ,  CHARLES, Marie-Hélène

IPC分类号： G01N33/543 ,  C07K17/00 ,  G01N33/532

CPC分类号： G01N33/54393 ,  C12N15/62 ,  C12N15/87

摘要： The invention concerns a method for isolating a target biological material contained in a sample, which consists in providing a capture phase comprising an organic molecule having at least a reactive function and at least a protein material capable of recognising or binding, specifically and directly or indirectly, with the target biological material, said protein material having a specific covalent binding site with the organic molecule reactive function, consisting of at least a tag comprising at least six contiguous lysine, or lysine derivative residues; the method consists in contacting said target biological material with at least the capture phase; and detecting the target biological material fixed on the capture phase: The invention also concerns the capture and detection phases, and a reagent containing them.

公开(公告)号：EP1212359A1

公开(公告)日：2002-06-12

申请号：EP00960783.9

申请日：2000-09-01

申请人： BIO MERIEUX ,  INSTITUT NATIONAL DE LA SANTE ET DE LARECHERCHE MEDICALE (INSERM)

发明人： MALLET, François ,  COSSET, François-Loic ,  BLOND, Jean-Luc ,  LAVILLETTE, Dimitri ,  BOUTON, Olivier ,  RUGGIERI, Alessia

IPC分类号： C07K14/15 ,  C12N15/48 ,  A61P35/00 ,  A61P15/00 ,  A61K39/21 ,  A61K48/00 ,  C12N5/10

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K48/00 ,  C12N2740/10022

摘要： The invention concerns a method for detecting the expression of an envelope protein or polypeptide of a human endogenous retrovirus, characterised in that the protein or polypeptide has a polypeptide sequence comprising the sequence SEQ ID NO: 1 or a fragment of SEQ ID NO: 1 or a sequence having, for every sequence of 20 amino acids, at least 90 % identity with the SEQ ID NO: 1 or with a fragment of SEQ ID NO: 1, and the method consists in detecting the fusogenic power of said protein or said fragment in the cells of a cell tissue or cell culture, by demonstrating the formation of syncytia. A gene or a nucleic acid or a fragment thereof is used for preparing a therapeutic or prophylactic composition, in particular for treating cancers and for preventing deficiency in placental development.

公开(公告)号：EP0975968A2

公开(公告)日：2000-02-02

申请号：EP98921550.4

申请日：1998-04-16

申请人： BIO MERIEUX

发明人： ELAISSARI, Abdelhamid ,  DURACHER, David ,  PICHOT, Christian ,  MALLET, François ,  NOVELLI-ROUSSEAU, Armelle

IPC分类号： G01N33/543

CPC分类号： C08F220/54 ,  G01N33/54313 ,  G01N33/54326 ,  G01N33/54353

摘要： The invention concerns a method for isolating a target biological material contained in a sample, consisting in the following steps: providing a capture phase, in microparticulate or linear form, consisting of at least a first particulate or linear polymer, with apparent hydrophile character and first complexing groups, the latter being bound by co-ordination to a first transition metal, which is itself bound to a first biological entity capable of specifically recognising the target biological material; contacting said target biological material with at least the capture phase; and detecting the capture phase-target biological material complex, optionally with a detection phase, in microparticulate or linear form, and consisting of at least a second particulate or linear polymer, with apparent hydrophile character and second complexing groups, the latter being bound by co-ordination to a second transition metal, which is itself bound to a second biological entity capable of specifically recognising the target biological material, and a marker.

公开(公告)号：EP1070256B1

公开(公告)日：2004-09-22

申请号：EP99913389.5

申请日：1999-04-12

申请人： BIO MERIEUX

发明人： MALLET, François ,  THERETZ, Alain ,  PEROUZEL, Eric ,  HEBRARD, Christine

IPC分类号： G01N33/552 ,  G01N33/551

CPC分类号： G01N33/552 ,  Y10S977/953

摘要： The invention concerns a method for fixing a biological molecule, associated with a specific binding site, on the surface of a support made of silica or metal oxide. The invention also concerns a surface functionalised by the method and the use of such a surface. The method is characterised in that it consists in: functionalising the surface by cleaning, using at least a solvent or an oxygen plasma or any other process for forming alcohol groups on the support surface, to make it hydrophilic; contacting the biological molecule on said functionalised surface; and functionalising the support by fixing the biological molecule specific binding site on at least one of the support alcohol groups. The invention is particularly useful in the field of biomedical diagnosis.

公开(公告)号：EP0486661A1

公开(公告)日：1992-05-27

申请号：EP91911286.0

申请日：1991-06-11

申请人： BIO MERIEUX, Société anonyme

发明人： CROS, Philippe ,  ALLIBERT, Patrice ,  MALLET, François ,  MABILAT, Claude ,  MANDRAND, Bernard

IPC分类号： C12N15 ,  C12Q1

CPC分类号： C12Q1/6883 ,  C12Q1/6813 ,  C12Q1/6834 ,  C12Q1/6886 ,  C12Q1/689 ,  C12Q1/703 ,  C12Q1/708

摘要： Procédé de détection d'une séquence nucléotidique simple brin dans un échantillon la contenant ou susceptible de la contenir, selon la technique d'hybridation sandwich comportant une étape d'incubation de l'échantillon avec une sonde de capture fixée de façon passive sur un support solide et une sonde de détection marquée avec un marqueur non radioactif, les sondes de capture et de détection étant capables d'hybridation, respectivement, avec deux régions non chevauchantes de la séquence nucléotidique-cible recherchée, et l'étape d'incubation étant suivie d'une étape de lavage pour éliminer les réactifs non fixés par hybridation, caractérisé par le fait que la sonde de capture contient de 9 à 30 nucléotides et est fixée sur ledit support solide constitué par un matériau à base d'un composé hydrophobe. Application notamment au diagnostic des maladies infectieuses ou génétiques ainsi qu'au typage cellulaire.

摘要翻译： 根据三明治式杂交技术，检测含有它或能够含有它的样品中单链核苷酸序列的方法，所述方法包括将样品与捕获探针孵育的步骤，所述捕获探针被动地固定在载体上 固体和用非放射性标记物标记的检测探针，捕获和检测探针分别能够与靶核苷酸靶序列的两个非重叠区域杂交，并且遵循孵育步骤 的用于通过杂交除去未固定的试剂的洗涤步骤，其特征在于所述捕获探针含有9至30个核苷酸并且固定在由基于疏水性化合物的材料组成的所述固体载体上。 特别适用于传染病或遗传疾病的诊断以及细胞分型。

公开(公告)号：EP1000158A1

公开(公告)日：2000-05-17

申请号：EP98935106.9

申请日：1998-07-06

申请人： BIO MERIEUX

发明人： BESEME, Frédéric ,  BLOND, Jean-Luc ,  BOUTON, Olivier ,  MANDRAND, Bernard ,  MALLET, François ,  PERRON, Herve

IPC分类号： C12N15/48 ,  C07K14/15 ,  C12Q1/68 ,  C07K16/10 ,  G01N33/569

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C12N2740/10022 ,  G01N33/564

摘要： The invention concerns a genomic retroviral nucleic material, in isolated or purified state, at least partially functional or non-functional, whereof the genome comprises a reference nucleotide sequence selected from the group including sequences SEQ ID Nos: 1 to 15, their complementary sequences, and their equivalent sequences, in particular the nucleotide sequences having, for every series of 100 contiguous monomers, at least 70 % and preferably at least 90 % homology with said sequences SEQ ID Nos: 1 to 15. The invention also concerns the application of said material.

公开(公告)号：EP0996731A2

公开(公告)日：2000-05-03

申请号：EP98936467.4

申请日：1998-07-07

申请人： BIO MERIEUX

发明人： PARAHNOS-BACCALA, Glaucia ,  KOMURIAN-PRADEL, Florence ,  BEDIN, Frédéric ,  SODOYER, Mireille ,  OTT, Catherine ,  MALLET, François ,  PERRON, Hervé ,  MANDRAND, Bernard

IPC分类号： C12N15/48 ,  C12Q1/70 ,  C07K14/15 ,  A61K31/70

CPC分类号： C07K14/4713 ,  C07K14/005 ,  C12N2740/10022

摘要： The invention concerns a nucleic material, in isolated or purified state, and a nucleotide fragment comprising a nucleotide sequence selected from the group consisting in (i) the sequences SEQ ID NO: 112, SEQ ID NO:114, SEQ ID NO: 117, SEQ ID NO: 120, SEQ ID NO. 124, SEQ ID NO: 130, SEQ ID NO: 141 and SEQ ID NO: 142, (ii) the complementary sequences of sequences (i); and (iii) the sequences equivalent to sequences (ii) and (iii), in particular the sequence having for every series of 100 contiguous monomers, at least 50 %, preferably 70 % homology with sequences (i) and (ii) respectively. The invention also concerns their uses for detecting a retrovirus associated with multiple sclerosis and/or rheumatoid arthritis.

公开(公告)号：EP1212359B1

公开(公告)日：2011-11-02

申请号：EP00960783.9

申请日：2000-09-01

申请人： BIO MERIEUX ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)

发明人： MALLET, François ,  COSSET, François-Loic ,  BLOND, Jean-Luc ,  LAVILLETTE, Dimitri ,  BOUTON, Olivier ,  RUGGIERI, Alessia

IPC分类号： C07K14/15 ,  C12N15/48 ,  A61P35/00 ,  A61P15/00 ,  A61K39/21 ,  A61K48/00 ,  C12N5/10

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K48/00 ,  C12N2740/10022

摘要： The invention concerns a method for detecting the expression of an envelope protein or polypeptide of a human endogenous retrovirus, characterised in that the protein or polypeptide has a polypeptide sequence comprising the sequence SEQ ID NO: 1 or a fragment of SEQ ID NO: 1 or a sequence having, for every sequence of 20 amino acids, at least 90 % identity with the SEQ ID NO: 1 or with a fragment of SEQ ID NO: 1, and the method consists in detecting the fusogenic power of said protein or said fragment in the cells of a cell tissue or cell culture, by demonstrating the formation of syncytia. A gene or a nucleic acid or a fragment thereof is used for preparing a therapeutic or prophylactic composition, in particular for treating cancers and for preventing deficiency in placental development.

公开(公告)号：EP1000158B1

公开(公告)日：2006-11-22

申请号：EP98935106.9

申请日：1998-07-06

申请人： BIO MERIEUX

发明人： BESEME, Frédéric ,  BLOND, Jean-Luc ,  BOUTON, Olivier ,  MANDRAND, Bernard ,  MALLET, François ,  PERRON, Herve

IPC分类号： C12N15/48 ,  C07K14/15 ,  C12Q1/68 ,  C07K16/10 ,  G01N33/569

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C12N2740/10022 ,  G01N33/564

摘要： The invention concerns a genomic retroviral nucleic material, in isolated or purified state, at least partially functional or non-functional, whereof the genome comprises a reference nucleotide sequence selected from the group including sequences SEQ ID Nos: 1 to 15, their complementary sequences, and their equivalent sequences, in particular the nucleotide sequences having, for every series of 100 contiguous monomers, at least 70 % and preferably at least 90 % homology with said sequences SEQ ID Nos: 1 to 15. The invention also concerns the application of said material.