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1.发明授权PROCEDE D'OBTENTION D'UNE CULTURE CELLULAIRE VIABLE INFECTEE PAR UN VIRUS ASSOCIE A LA SCLEROSE EN PLAQUES 失效用于生产生活培养细胞中被感染是一个多发性硬化症相关病毒
公开(公告)号：EP0592636B1
公开(公告)日：1998-11-25
申请号：EP93908990.0
申请日：1993-04-02
CPC分类号： G01N33/56983 , C07K14/005 , C07K16/10 , C12N7/00 , C12N15/85 , C12N2710/16622 , C12N2740/10021 , C12N2740/10022 , C12N2740/10051 , G01N2469/20
摘要： Process for the in vitro culture of cells infected by a multiple sclerosis associated virus, and infected cell lines so produced. According to the invention, the process consists: a) in cultivating human cells infected by a viral strain, obtaining at least one primary cell culture infected by said viral strain, b) cultivating non-infected human cells permissive to said viral strain, obtaining at least one permissive culture, c) cultivating together at least one sample of an infected primary cell culture and one sample of the permissive culture to obtain a first infected derived culture, d) cultivating in series the first infected derived culture. Application in the pharmaceutical diagnostic industry.
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2.发明公开PROCEDE D'OBTENTION D'UNE CULTURE CELLULAIRE VIABLE INFECTEE PAR UN VIRUS ASSOCIE A LA SCLEROSE EN PLAQUES 失效用于生产生活培养细胞中被感染多发性硬化症相关病毒ARE。
公开(公告)号：EP0592636A1
公开(公告)日：1994-04-20
申请号：EP93908990.0
申请日：1993-04-02
CPC分类号： G01N33/56983 , C07K14/005 , C07K16/10 , C12N7/00 , C12N15/85 , C12N2710/16622 , C12N2740/10021 , C12N2740/10022 , C12N2740/10051 , G01N2469/20
摘要： La présente invention a pour objet un procédé pour la culture in vitro de cellules infectées par un virus associé à la sclérose en plaques, et les lignées cellulaires infectées ainsi obtenues. Selon l'invention, le procédé consiste à: a) cultiver des cellules humaines infectées par une souche virale, pour obtenir au moins une culture de cellules primaires infectées par ladite souche virale, b) cultiver des cellules humaines non infectées et permissives à ladite souche virale, pour obtenir au moins une culture permissive, c) co-cultiver au moins un prélèvement d'une culture de cellules primaires infectées et un prélèvement de la culture permissive, pour obtenir une première culture dérivée infectée, d) cultiver en série la première culture dérivée infectée. L'invention trouve notamment application dans le domaine de l'industrie diagnostique pharmaceutique.
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3.发明授权THE LTR REGION OF MSRV-1 AND THE PROTEINS IT ENCODES, AND PROBES AND METHODS FOR DETECTING MSRV-1 RETROVIRUS 有权在LTR地区MSRV1以及由你所编码的蛋白质，探讨和方法检测MSRV1逆转录病毒的
公开(公告)号：EP1151108B1
公开(公告)日：2005-11-30
申请号：EP00902825.9
申请日：2000-02-15
申请人： BIO MERIEUX
CPC分类号： C07K14/005 , C12N2740/10022
摘要： The invention relates to a nucleotide fragment of a LTR-RU5 region comprising a nucleotide sequence which encodes the expression of a protein, wherein said protein comprises a peptide sequence selected from SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4, and a complementary nucleotide fragment; a probe and a primer hybridizing with said fragment; a protein encoded by said fragment and a polypeptide; an antibody directed against said protein or said polypeptide; and a process for detecting the presence of MSRV-1 retrovirus using a probe or an antibody of the invention.
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4.发明公开PROCEDE ET MILIEU DE CULTURE POUR L'OBTENTION DE CELLULES INFECTEES PAR UN VIRUS ASSOCIE A LA SCLEROSE EN PLAQUES 失效多发性硬化症，病毒制造方法和培养基相关感染的细胞中获得的。
公开(公告)号：EP0587873A1
公开(公告)日：1994-03-23
申请号：EP93908989.0
申请日：1993-04-02
申请人： BIO MERIEUX
CPC分类号： G01N33/56983 , C07K14/005 , C07K16/10 , C12N7/00 , C12N15/85 , C12N2710/16622 , C12N2740/10022 , C12N2740/10051 , G01N2469/20
摘要： Procédé d'obtention in vitro d'une culture ou lignée cellulaire infectée par une souche virale associée à la sclérose en plaques (SEP), selon lequel on effectue un prélèvement corporel sur un individu atteint de SEP, on cultive ledit prélèvement dans un milieu de culture favorisant la croissance des cellules infectées, pour obtenir une culture de cellules primaires infectées, et on cultive en série, c'est-à-dire par passages successifs, dans ledit milieu de culture, un prélèvement de la culture de cellules primaires, ou d'une sous-culture de cette dernière, pour obtenir la culture ou lignée cellulaire infectée par un virus associé à la SEP, caractérisé en ce que le milieu de culture contient en outre un anticorps anti-interféron béta, ou dirigé contre une molécule antigéniquement proche, jouant un rôle inhibiteur dans l'expression virale, permettant d'exprimer de manière persistante et de propager la souche virale dans la culture ou lignée cellulaire.
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5.发明授权DETECTION D'UNE ACTIVITE SUPERANTIGENIQUE INDUITE PAR MSRV-1 DANS UN ECHANTILLON BIOLOGIQUE 有权一个MSRV1超抗原诱导活性在生物样品检测
公开(公告)号：EP1163522B1
公开(公告)日：2006-11-22
申请号：EP00912720.0
申请日：2000-03-20
申请人： BIO MERIEUX
发明人： PERRON, Hervé , LAFONT, Monique
IPC分类号： G01N33/569 , G01N33/68 , G01N33/50 , G01N33/564 , G01N33/566 , G01N33/574 , A61K39/00
CPC分类号： G01N33/5047 , G01N33/5008 , G01N33/5023 , G01N33/5091 , G01N33/564 , G01N33/56977 , G01N33/6893 , G01N2510/00
摘要： The invention concerns in particular a method for detecting superantigen activity in a biological sample, which consists in: demonstrating a majority spread rate of lymphocytes bearing a V beta 16 and/or V beta 17 determinant or a majority loss rate of lymphocytes bearing a V beta 16 determinant and a method for detecting a pathological condition in a biological sample which consists in demonstrating at least one of the following parameters: a superantigen activity; a stimulation of the production of cytokines such as IL-6 and INF- gamma ; induced cell apoptosis.
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6.发明公开
公开(公告)号：EP0975747A1
公开(公告)日：2000-02-02
申请号：EP98922903.4
申请日：1998-04-29
申请人： BIO MERIEUX
IPC分类号： C12N15/10 , C07K14/47 , C07K14/15 , C07K7/06 , C07K7/08 , G01N33/564 , G01N33/569
CPC分类号： C07K7/06 , A61K39/00 , C07K5/1013 , C07K5/1021 , C07K7/08 , C07K14/005 , C07K14/4713 , C12N2740/10022 , Y10S435/81 , Y10S435/975
摘要： The invention concerns a polypeptide specifically reacting with the antibodies of patients suffering from multiple sclerosis (SEP) and whereof the peptide sequence comprises at least one sequence selected among SEQ ID No: 1 to SEQ ID No: 19, and their equivalent sequences, and the use of this polypeptide in a reagent and a kit for detecting multiple sclerosis, an immunoreactive composition and in a method for fixing, in a biological sample, antibodies characteristic and/or specific of multiple sclerosis.
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7.发明授权PROCEDE POUR L'OBTENTION DE CELLULES INFECTEES PAR UN VIRUS ASSOCIE A LA SCLEROSE EN PLAQUES 失效多发性硬化症，病毒制造方法相关感染的细胞来获得
公开(公告)号：EP0587873B1
公开(公告)日：2000-01-19
申请号：EP93908989.2
申请日：1993-04-02
申请人： BIO MERIEUX
CPC分类号： G01N33/56983 , C07K14/005 , C07K16/10 , C12N7/00 , C12N15/85 , C12N2710/16622 , C12N2740/10022 , C12N2740/10051 , G01N2469/20
摘要： Process for in vitro production of a culture or cell line infected by a viral strain associated with multiple sclerosis. According to the process, a sample is taken from a person with multiple sclerosis, the sample is cultivated in a medium promoting the growth of infected cells to obtain a culture of primary infected cells. A sample of the primary cell culture or a sub-culture of the latter is then cultivated in series, i.e. by means of successive cultures, in said culture medium to obtain the culture or cell line infected by a multiple sclerosis associated virus. The invention is characterized in that the culture medium further contains a beta anti-interferon antibody or antibody which is directed against an antigenically close molecule. Said antibody plays an inhibiting role in viral expression, has long-lasting expression and propagates the viral strain in the culture or cell line.
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8.发明授权VIRAL MATERIAL AND NUCLEOTIDE FRAGMENTS ASSOCIATED WITH MULTIPLE SCLEROSIS, FOR DIAGNOSTIC, PROPHYLACTIC AND THERAPEUTIC PURPOSES 失效病毒物质和多发性硬化具有诊断预防性核苷酸片段，治疗用途和相关联的
公开(公告)号：EP0942987B1
公开(公告)日：2009-08-19
申请号：EP97911411.3
申请日：1997-11-26
申请人： BIO MERIEUX
发明人： PERRON, Hervé , BESEME, Frédéric , BEDIN, Frédéric , PARANHOS-BACCALA, Glaucia , KOMURIAN-PRADEL, Florence , JOLIVET-REYNAUD, Colette , MANDRAND, Bernard
IPC分类号： C12N15/48 , C12N15/08 , C12N7/02 , C07K14/15 , C12N9/12 , C12N9/22 , C12Q1/70 , C07K16/10 , G01N33/569 , A61K39/21 , A61K39/42 , A61K48/00
CPC分类号： C07K14/005 , A61K38/00 , C12N7/00 , C12N9/1241 , C12N2740/10022 , C12N2740/10051 , C12Q1/702
摘要： The invention relates to a nucleic material, in the isolated or purified state, comprising a nucleotide sequence selected from the group including sequences SEQ ID NO:93, SEQ ID NO:94, their complementary sequences and their equivalent sequences, in particular nucleotide sequences displaying, for any succession of 100 contiguous monomers, at least 50 % and preferably at least 60 % homology with said sequences SEQ ID NO:93, SEQ ID NO:94 and their complementary sequences, excluding HSERV-9 sequence.
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9.发明授权MATERIEL NUCLEIQUE RETROVIRAL ET FRAGMENTS NUCLEOTIDIQUES NOTAMMENT ASSOCIES A LA SCLEROSE EN PLAQUES ET/OU LA POLYARTHRITE RHUMATOIDE, A DES FINS DE DIAGNOSTIC, PROPHYLACTIQUES ET THERAPEUTIQUES 失效诊断，预防与治疗多发性硬化和/或风湿性多关节炎ASSOCIATED RETRO病毒核酸和相关核苷酸片段
公开(公告)号：EP0996731B1
公开(公告)日：2005-08-31
申请号：EP98936467.4
申请日：1998-07-07
申请人： BIO MERIEUX
发明人： PARAHNOS-BACCALA, Glaucia , KOMURIAN-PRADEL, Florence , BEDIN, Frédéric , SODOYER, Mireille , OTT, Catherine , MALLET, François , PERRON, Hervé , MANDRAND, Bernard
CPC分类号： C07K14/4713 , C07K14/005 , C12N2740/10022
摘要： The invention concerns a nucleic material, in isolated or purified state, and a nucleotide fragment comprising a nucleotide sequence selected from the group consisting in (i) the sequences SEQ ID NO: 112, SEQ ID NO:114, SEQ ID NO: 117, SEQ ID NO: 120, SEQ ID NO. 124, SEQ ID NO: 130, SEQ ID NO: 141 and SEQ ID NO: 142, (ii) the complementary sequences of sequences (i); and (iii) the sequences equivalent to sequences (ii) and (iii), in particular the sequence having for every series of 100 contiguous monomers, at least 50 %, preferably 70 % homology with sequences (i) and (ii) respectively. The invention also concerns their uses for detecting a retrovirus associated with multiple sclerosis and/or rheumatoid arthritis.
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10.发明公开
公开(公告)号：EP1163522A1
公开(公告)日：2001-12-19
申请号：EP00912720.0
申请日：2000-03-20
申请人： BIO MERIEUX
发明人： PERRON, Hervé , LAFONT, Monique
IPC分类号： G01N33/569 , G01N33/68 , G01N33/50 , G01N33/564 , G01N33/566 , G01N33/574 , A61K39/00
CPC分类号： G01N33/5047 , G01N33/5008 , G01N33/5023 , G01N33/5091 , G01N33/564 , G01N33/56977 , G01N33/6893 , G01N2510/00
摘要： The invention concerns in particular a method for detecting superantigen activity in a biological sample, which consists in: demonstrating a majority spread rate of lymphocytes bearing a Vβ 16 and/or Vβ17 determinant or a majority loss rate of lymphocytes bearing a Vβ16 determinant and a method for detecting a pathological condition in a biological sample which consists in demonstrating at least one of the following parameters: a superantigen activity; a stimulation of the production of cytokines such as IL-6 and INF-η; induced cell apoptosis.
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