公开(公告)号：EP2277034A1

公开(公告)日：2011-01-26

申请号：EP09747252.6

申请日：2009-05-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： PRICE, Glenn ,  PANATTONI, Cory ,  ROWELL, Craig ,  PETERSEN, Shane ,  PEREZ, Evelio

IPC: G01N27/26 ,  G01N27/447

CPC classification number: G01N27/453 ,  G01N27/44704 ,  G01N27/44756

Abstract: A slab gel cassette is formed from generally flat plates welded together along their borders with a gel space between the plates that is open at the top and bottom edges of the plates to expose the opposing ends of a gel in the gel space to buffer solutions in which electrodes are immersed. The plates are shaped such that the weld line does not intersect with the bottom opening of the gel space. Sealing of the bottom of the gel space, which is necessary for gel casting, is achieved by placing a sealing strip over the bottom opening with no danger of leakage of the gel solution along a weld line.

公开(公告)号：EP2820676A1

公开(公告)日：2015-01-07

申请号：EP13755695.7

申请日：2013-01-14

Applicant: Bio-rad Laboratories, Inc.

Inventor： HILLENDAHL, James W. ,  PEREZ, Evelio

IPC: H01L27/00

CPC classification number: F21V21/005 ,  F21V9/083 ,  F21V13/02 ,  G01N21/255 ,  G01N21/6452 ,  G01N21/6456 ,  G01N27/447 ,  G01N2021/6463 ,  G01N2021/6471 ,  G01N2201/06 ,  G01N2201/0626 ,  G01N2201/0635

Abstract: A planar sample, particularly of the type used in biological laboratories for detection and sometimes analysis of two-dimensional arrays of proteins, nucleic acids, or other biological species, is illuminated by epi-illumination using optically filtered line lights that are arranged along opposing parallel sides of a rectangle in which the sample array resides, with two coaxial line lights on each side of the rectangle, and the two on any given side being separated by a gap whose optimal width depends on the wavelength band transmitted by the optical filter. Surprisingly, the gap eliminates the peak in intensity at the center of the sample area and the decrease that occurs from the center outward that would otherwise occur with a single continuous filtered line light, producing instead a substantially uniform intensity along the direction parallel to the line lights.

Abstract translation: 平面样品，特别是用于生物实验室中用于检测蛋白质，核酸或其他生物物种的二维阵列以及有时分析蛋白质，核酸或其他生物物种的二维阵列的类型的平面样品，使用光学滤波的线光照射，所述光学滤波的线光沿着相对平行 样本阵列所在的矩形的两侧，在矩形的每一侧上具有两个同轴线光源，并且在任何给定侧上的两个由间隙隔开，该间隙的最佳宽度取决于由光学滤波器传输的波长带。 令人惊讶的是，该间隙消除了样品区域中心处的强度峰值以及从中心向外发生的减少，否则将发生在单个连续的过滤后的线光下，而是沿平行于线的方向产生基本上均匀的强度 灯。

公开(公告)号：EP1277048A1

公开(公告)日：2003-01-22

申请号：EP01924783.2

申请日：2001-04-06

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： PEREZ, Evelio

IPC: G01N27/447

CPC classification number: G01N27/44704

Abstract: Parallel plate slab gel enclosures (slab gel cassettes) used for vertical slab gel electrophoresis are secured to a frame (14) to form a chamber for an upper buffer solution, and the securement is achieved by a pair of clamps (16, 17) that compress the cassettes (12, 13) against the frame. The clamps are preferably mounted to the frame in a pivotal connection that enables the user to easily rotate the clamps in and out of their clamping positions. An additional preferred feature is a pin protruding from each clamp in a position causing the pin to move upward against the bottom of the cassette and push the cassette upwards as the clamp is being engaged. This is particularly useful for cassettes formed of plates of unequal height where the shorter plate fits under an inverse shoulder on a gasket which thereby seals against both plates.

公开(公告)号：EP1153287A1

公开(公告)日：2001-11-14

申请号：EP00907279.4

申请日：2000-02-10

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： PEREZ, Evelio ,  FERNWOOD, George

IPC: G01N27/447

CPC classification number: G01N27/44704 ,  G01N27/44743

Abstract: Flat plates serving as molds and enclosures for slab gels are held together by a clamping frame in which the side edges of the plates slide into facing channels and are clamped by lever-operated cams that compress the channel walls against the plate surfaces. The joined plates are held on a vertical support rack containing a finger-operated spring-loaded notched clamp that presses down on the plates to seal the opening at the bottom edges of the plates against a gasket. A well-forming comb for insertion between the plates contains flexible outwardly angled fingers to seal against the spacers between the plates.

Abstract translation: 用作平板凝胶的模具和外壳的平板通过夹紧框架保持在一起，其中平板的侧边缘滑入面对的通道并且由杠杆操作的凸轮夹紧，凸轮将通道壁压靠在板表面上。 被连接的板被保持在一个垂直支撑架上，该支撑架包含一个手指操作的弹簧加载的带凹口的夹子，该夹子向下压在板上以密封板的底部边缘处的开口并抵靠垫圈。 用于在板之间插入的良好成形梳子包含柔性向外成角度的指状物以密封板之间的间隔物。

公开(公告)号：EP2987019A2

公开(公告)日：2016-02-24

申请号：EP14832584.8

申请日：2014-07-28

Applicant: Bio-rad Laboratories, Inc.

Inventor： SWIHART, Steve ,  THRUSH, Evan ,  PEREZ, Evelio ,  MCDONALD, Kevin

IPC: G02B26/08

CPC classification number: G02B15/14

Abstract: An imaging assembly for the viewing, imaging, and analysis of chemiluminescent or bioluminescent samples in gels or other substrates, in which an adjustable camera and lens module having a prime or fixed lens or a focusing lens is moved to change the field of view by shifting the focal plane of the camera and lens module. The imaging assembly can also include a mirror to bend or fold the optical path between the camera and lens module and the target area having a sample, in which the mirror can move in the same vertical direction as the camera and lens module. Further, the camera and lens module can be configured to more move in a diagonal direction relative to the location of the imaging target area. The imaging assembly can further have a duct system adaptable to adjust with the movement of the camera and lens module.

Abstract translation: 用于观察，成像和分析凝胶或其他基底中的化学发光或生物发光样品的成像组件，其中具有主要或固定透镜或聚焦透镜的可调节相机和透镜模块被移动以通过移动来改变视场 相机和镜头模块的焦平面。 成像组件还可以包括反射镜，以在照相机和镜头模块与具有样品的目标区域之间弯曲或折叠光路，其中反射镜可以在与照相机和镜头模块相同的垂直方向上移动。 此外，相机和镜头模块可以被配置为相对于成像目标区域的位置在对角线方向上更多地移动。 成像组件可以进一步具有适于随照相机和镜头模块的移动而调整的管道系统。

公开(公告)号：EP1315961B1

公开(公告)日：2011-11-02

申请号：EP01968542.9

申请日：2001-09-04

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： FERNWOOD, George ,  WHITMAN, Christina ,  HARBERS, Adriana, J. ,  PEREZ, Evelio

IPC: G01N27/447

CPC classification number: G01N27/44704 ,  G01N27/44782

Abstract: Electrophoresis is performed simultaneously in a multitude of slab gels in a single cell that supports the slab gels vertically and parallel to each other while immersed in a buffer solution, and that applies a voltage to all gels simultaneously through a single pair of plate electrodes. Temperature control is achieved by circulating the buffer solution upward through the cell, entering near the base of each slab gel and leaving near the top, and cooling the circulating buffer solution with a tube heat exchanger positioned on the floor of the cell.

公开(公告)号：EP1277048B1

公开(公告)日：2011-06-08

申请号：EP01924783.2

申请日：2001-04-06

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： PEREZ, Evelio

IPC: G01N27/447

CPC classification number: G01N27/44704

Abstract: Parallel plate slab gel enclosures (slab gel cassettes) used for vertical slab gel electrophoresis are secured to a frame (14) to form a chamber for an upper buffer solution, and the securement is achieved by a pair of clamps (16, 17) that compress the cassettes (12, 13) against the frame. The clamps are preferably mounted to the frame in a pivotal connection that enables the user to easily rotate the clamps in and out of their clamping positions. An additional preferred feature is a pin protruding from each clamp in a position causing the pin to move upward against the bottom of the cassette and push the cassette upwards as the clamp is being engaged. This is particularly useful for cassettes formed of plates of unequal height where the shorter plate fits under an inverse shoulder on a gasket which thereby seals against both plates.

Abstract translation: 用于垂直平板凝胶电泳的平行板平板凝胶盒（平板凝胶盒）固定到框架（14）上以形成用于上部缓冲溶液的腔室，并且通过一对夹具（16,17）实现固定，所述夹具 将盒（12,13）压靠在框架上。 夹具优选地以枢转连接的方式安装到框架上，使得使用者能够容易地将夹具旋入和移出其夹紧位置。 另一个优选的特征是从每个夹具突出的销钉在一个位置，使得销钉向上抵靠在盒子的底部上并且在夹具被接合时向上推动盒子。 这对于由不相等高度的板形成的盒子是特别有用的，其中较短的板安装在衬垫上的相反肩部下方，从而密封两个板。

公开(公告)号：EP1153287B1

公开(公告)日：2007-04-11

申请号：EP00907279.4

申请日：2000-02-10

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： PEREZ, Evelio ,  FERNWOOD, George

IPC: G01N27/447

CPC classification number: G01N27/44704 ,  G01N27/44743

Abstract: Flat plates serving as molds and enclosures for slab gels are held together by a clamping frame in which the side edges of the plates slide into facing channels and are clamped by lever-operated cams that compress the channel walls against the plate surfaces. The joined plates are held on a vertical support rack containing a finger-operated spring-loaded notched clamp that presses down on the plates to seal the opening at the bottom edges of the plates against a gasket. A well-forming comb for insertion between the plates contains flexible outwardly angled fingers to seal against the spacers between the plates.

公开(公告)号：EP3427101B1

公开(公告)日：2020-11-18

申请号：EP17764106.5

申请日：2017-03-09

Applicant: Bio-Rad Laboratories, Inc.

Inventor： PEREZ, Evelio ,  THRUSH, Evan P. ,  SWIHART, Stephen L.R. ,  HALL, Brian D.

IPC: G02B21/00 ,  G02B21/26 ,  G02B21/36 ,  G02B23/00 ,  G03B17/02 ,  G03B17/55 ,  G03B17/56 ,  G03B43/00 ,  H04N5/225

公开(公告)号：EP2987006A1

公开(公告)日：2016-02-24

申请号：EP14842718.0

申请日：2014-09-04

Applicant: Bio-Rad Laboratories, Inc.

Inventor： THRUSH, Evan ,  PEREZ, Evelio ,  SWIHART, Steve

IPC: G02B5/08 ,  G02B5/18 ,  G02B7/182

CPC classification number: G02B21/361 ,  G01N21/00 ,  G01N21/76 ,  G02B17/023 ,  G02B21/0008 ,  G03B15/00 ,  G03B17/17

Abstract: An imaging assembly for the viewing, imaging, and analysis of biological, chemical, and/or biochemical samples in gels or other substrates, in which an adjustable camera and lens module, a reflex mirror, and a focal plane mirror, are configured to bend or fold an optical path in order to image a target region, and where the optical path can be reflected along non-orthogonal angles. The imaging assembly is configured to reduce the overall size of the imaging apparatus due to the angles at which the mirrors and camera and lens assembly are positioned relative to each other, which allows for the imaging of relatively larger samples in the target region.

Abstract translation: 一种成像组件，用于凝胶或其他基质中的生物，化学和/或生物化学样品的观察，成像和分析，其中可调节的照相机和镜头模块，反射镜和焦平面镜被配置为弯曲 或折叠光路以便成像目标区域，并且其中光路可以沿着非正交角度被反射。 成像组件被配置成由于镜子和照相机和透镜组件相对于彼此定位的角度而减小成像装置的整体尺寸，这允许成像目标区域中的相对较大的样本。