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    MECHANICAL ZOOM IMAGING APPARATUS
    2.
    发明公开
    MECHANICAL ZOOM IMAGING APPARATUS 审中-公开
    机械变焦成像设备

    公开(公告)号：EP2987019A2

    公开(公告)日：2016-02-24

    申请号：EP14832584.8

    申请日：2014-07-28

    Applicant: Bio-rad Laboratories, Inc.

    Inventor： SWIHART, Steve ,  THRUSH, Evan ,  PEREZ, Evelio ,  MCDONALD, Kevin

    IPC: G02B26/08

    CPC classification number: G02B15/14

    Abstract: An imaging assembly for the viewing, imaging, and analysis of chemiluminescent or bioluminescent samples in gels or other substrates, in which an adjustable camera and lens module having a prime or fixed lens or a focusing lens is moved to change the field of view by shifting the focal plane of the camera and lens module. The imaging assembly can also include a mirror to bend or fold the optical path between the camera and lens module and the target area having a sample, in which the mirror can move in the same vertical direction as the camera and lens module. Further, the camera and lens module can be configured to more move in a diagonal direction relative to the location of the imaging target area. The imaging assembly can further have a duct system adaptable to adjust with the movement of the camera and lens module.

    Abstract translation: 用于观察，成像和分析凝胶或其他基底中的化学发光或生物发光样品的成像组件，其中具有主要或固定透镜或聚焦透镜的可调节相机和透镜模块被移动以通过移动来改变视场 相机和镜头模块的焦平面。 成像组件还可以包括反射镜，以在照相机和镜头模块与具有样品的目标区域之间弯曲或折叠光路，其中反射镜可以在与照相机和镜头模块相同的垂直方向上移动。 此外，相机和镜头模块可以被配置为相对于成像目标区域的位置在对角线方向上更多地移动。 成像组件可以进一步具有适于随照相机和镜头模块的移动而调整的管道系统。

    STAIN-FREE PROTEIN QUANTIFICATION AND NORMALIZATION
    5.
    发明授权
    STAIN-FREE PROTEIN QUANTIFICATION AND NORMALIZATION 有权
    无污染蛋白质量化和标准化

    公开(公告)号：EP2841927B1

    公开(公告)日：2018-01-17

    申请号：EP13780565.1

    申请日：2013-04-25

    Applicant: Bio-rad Laboratories, Inc.

    Inventor： FREEBY, Steve ,  LIU, Ning ,  MCDONALD, Kevin ,  PAULUS, Aran ,  POSCH, Anton

    IPC: G01N33/68 ,  G01N21/64 ,  G06F19/20

    CPC classification number: G01N33/53 ,  G01N33/6827 ,  G06F19/10 ,  G06T7/0012 ,  G06T11/60 ,  G06T2207/30004

    Abstract: Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

    STAIN-FREE PROTEIN QUANTIFICATION AND NORMALIZATION
    6.
    发明公开
    STAIN-FREE PROTEIN QUANTIFICATION AND NORMALIZATION 有权
    FLECKENFREI PROTEINQUANTIFIZIERUNG UND NORMALISIERUNG

    公开(公告)号：EP2841927A1

    公开(公告)日：2015-03-04

    申请号：EP13780565.1

    申请日：2013-04-25

    Applicant: Bio-rad Laboratories, Inc.

    Inventor： FREEBY, Steve ,  LIU, Ning ,  MCDONALD, Kevin ,  PAULUS, Aran ,  POSCH, Anton

    IPC: G01N21/64 ,  G01N21/76 ,  G01N21/33 ,  G01N33/52 ,  G01N33/68 ,  G06F19/20 ,  G01J1/18 ,  G01J3/443 ,  C12Q1/06

    CPC classification number: G01N33/53 ,  G01N33/6827 ,  G06F19/10 ,  G06T7/0012 ,  G06T11/60 ,  G06T2207/30004

    Abstract: Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

    Abstract translation: 本文公开了使用卤代烷基化色氨酸荧光进行蛋白质定量和归一化的方法。 使用与色氨酸残基反应形成荧光产物的卤素取代的有机化合物（即卤代烷烃）处理复合蛋白质样品，即每种含有来自不同来源的不具有共同蛋白质谱的1,000个或更多个不同蛋白质的样品。 然后使用紫外线照射样品和每个样品中所有蛋白质产生的荧光发射的检测和定量，以获得各种样品中总蛋白质含量的比较值。 发现这些值被认为是比较总蛋白质含量的有效指示，尽管事实上，任何单个样品中的各种蛋白质之间的色氨酸水平变化很大，并且样品由于其起源的多样性而在它们之间趋于不同 在它们所含的蛋白质的身份和相对量中。 蛋白质样品也通过使用每个样品中的总蛋白的无污染检测或每个样品中的子样品检测来校正样品稀释度，样品加载和蛋白质转移不一致性的差异。

    IMAGE ACQUISITION FOR CHEMILUMINESCENT SAMPLES
    7.
    发明公开
    IMAGE ACQUISITION FOR CHEMILUMINESCENT SAMPLES 审中-公开
    图片化学发光样品

    公开(公告)号：EP2825867A1

    公开(公告)日：2015-01-21

    申请号：EP13761630.6

    申请日：2013-03-14

    Applicant: Bio-rad Laboratories, Inc.

    Inventor： KOTCHOU, Keith ,  MCDONALD, Kevin ,  LEE, James

    IPC: G01N21/76

    Abstract: An image system for detecting chemiluminescence in a sample uses a highly binned, short exposure initial image to calculate the exposure time for a final image of the sample. After calculation of the exposure time, at least two final images are taken, with saturated pixels removed and replaced in a first image with corresponding unsaturated pixels from a second image. The corresponding pixels are adjusted to reflect the different intensity levels between the first and second images, and the first image becomes the final image reflecting the detected chemiluminescence.

    CALIBRATION OF IMAGING DEVICE FOR BIOLOGICAL/CHEMICAL SAMPLES
    8.
    发明公开
    CALIBRATION OF IMAGING DEVICE FOR BIOLOGICAL/CHEMICAL SAMPLES 审中-公开
    KALIBRIERUNG EINER BILDGEBUNGSVORRICHTUNGFÜR生物学/ CHEMISCHE PROBEN

    公开(公告)号：EP2438554A1

    公开(公告)日：2012-04-11

    申请号：EP10783936.7

    申请日：2010-06-01

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： KOTCHOU, Keith ,  SCHULTZ, Christof ,  MCDONALD, Kevin ,  BHATT, Neeraj

    IPC: G06K9/00

    CPC classification number: G06T7/80 ,  G06T2207/30024 ,  G06T2207/30208

    Abstract: Methods, apparatuses, and systems for imaging biological/chemical samples are provided. A calibrated imaging system can allow a user to obtain an optimal focus setting (position) for any effective distance (e.g. a zoom setting). The optimal focus can be determined from a functional approximation that defines a relationship between effective distance and focus setting. A user can input a size, and an imaging system can determine the appropriate effective distance and focus. An imaging system can also determine a size based on any effective distance. A flat-field correction can also be determined for any effective distance or size.

    Abstract translation: 提供了用于成像生物/化学样品的方法，装置和系统。 校准的成像系统可以允许用户获得任何有效距离（例如，变焦设置）的最佳焦点设置（位置）。 可以从定义有效距离和焦点设置之间的关系的函数近似来确定最佳焦点。 用户可以输入尺寸，并且成像系统可以确定适当的有效距离和焦点。 成像系统还可以基于任何有效距离来确定尺寸。 对于任何有效的距离或大小，也可以确定平场校正。

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