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    STAIN-FREE PROTEIN QUANTIFICATION AND NORMALIZATION
    8.
    发明授权
    STAIN-FREE PROTEIN QUANTIFICATION AND NORMALIZATION 有权
    无污染蛋白质量化和标准化

    公开(公告)号：EP2841927B1

    公开(公告)日：2018-01-17

    申请号：EP13780565.1

    申请日：2013-04-25

    Applicant: Bio-rad Laboratories, Inc.

    Inventor： FREEBY, Steve ,  LIU, Ning ,  MCDONALD, Kevin ,  PAULUS, Aran ,  POSCH, Anton

    IPC: G01N33/68 ,  G01N21/64 ,  G06F19/20

    CPC classification number: G01N33/53 ,  G01N33/6827 ,  G06F19/10 ,  G06T7/0012 ,  G06T11/60 ,  G06T2207/30004

    Abstract: Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

    STAIN-FREE PROTEIN QUANTIFICATION AND NORMALIZATION
    9.
    发明公开
    STAIN-FREE PROTEIN QUANTIFICATION AND NORMALIZATION 有权
    FLECKENFREI PROTEINQUANTIFIZIERUNG UND NORMALISIERUNG

    公开(公告)号：EP2841927A1

    公开(公告)日：2015-03-04

    申请号：EP13780565.1

    申请日：2013-04-25

    Applicant: Bio-rad Laboratories, Inc.

    Inventor： FREEBY, Steve ,  LIU, Ning ,  MCDONALD, Kevin ,  PAULUS, Aran ,  POSCH, Anton

    IPC: G01N21/64 ,  G01N21/76 ,  G01N21/33 ,  G01N33/52 ,  G01N33/68 ,  G06F19/20 ,  G01J1/18 ,  G01J3/443 ,  C12Q1/06

    CPC classification number: G01N33/53 ,  G01N33/6827 ,  G06F19/10 ,  G06T7/0012 ,  G06T11/60 ,  G06T2207/30004

    Abstract: Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

    Abstract translation: 本文公开了使用卤代烷基化色氨酸荧光进行蛋白质定量和归一化的方法。 使用与色氨酸残基反应形成荧光产物的卤素取代的有机化合物（即卤代烷烃）处理复合蛋白质样品，即每种含有来自不同来源的不具有共同蛋白质谱的1,000个或更多个不同蛋白质的样品。 然后使用紫外线照射样品和每个样品中所有蛋白质产生的荧光发射的检测和定量，以获得各种样品中总蛋白质含量的比较值。 发现这些值被认为是比较总蛋白质含量的有效指示，尽管事实上，任何单个样品中的各种蛋白质之间的色氨酸水平变化很大，并且样品由于其起源的多样性而在它们之间趋于不同 在它们所含的蛋白质的身份和相对量中。 蛋白质样品也通过使用每个样品中的总蛋白的无污染检测或每个样品中的子样品检测来校正样品稀释度，样品加载和蛋白质转移不一致性的差异。

    ELECTRONIC PROTEIN FRACTIONATION
    10.
    发明公开
    ELECTRONIC PROTEIN FRACTIONATION 有权
    电子蛋白质分馏

    公开(公告)号：EP2834261A1

    公开(公告)日：2015-02-11

    申请号：EP13772164.3

    申请日：2013-03-19

    Applicant: Bio-rad Laboratories, Inc. ,  Technion Research & Development Foundation Ltd.

    Inventor： PAULUS, Aran ,  BOGOEV, Roumen ,  BROD, Elad ,  SIVAN, Uri

    IPC: C07K1/28

    CPC classification number: B01D61/54 ,  B01D61/42 ,  B01D61/445 ,  B01D61/46 ,  B01D2311/18 ,  C07K1/28 ,  G01N27/44795

    Abstract: Aspects of the invention are described throughout this document. In some embodiments, an apparatus is provided. In some embodiments, the apparatus comprises: a chamber divided into a first sub-chamber and a second sub-chamber by a dividing membrane, wherein the dividing membrane blocks or substantially blocks flow of fluid between the first and second sub-chamber; and wherein the first sub-chamber is in electrical communication with a first electrode; the second sub-chamber is in electrical communication with a second electrode; the first sub-chamber is in fluid communication with a first ion injector/extractor; the second sub-chamber is in fluid communication with a second ion injector/extractor; and and the second sub-chamber comprises an outlet.

    Abstract translation: 贯穿本文件描述了本发明的各方面。 在一些实施例中，提供了一种装置。 在一些实施例中，所述设备包括：通过分隔膜被分成第一子腔室和第二子腔室的腔室，其中所述分隔膜阻挡或基本阻塞所述第一和第二子腔室之间的流体流动; 并且其中所述第一子室与第一电极电连通; 第二子腔室与第二电极电连通; 第一子室与第一离子注射器/提取器流体连通; 第二子室与第二离子注射器/提取器流体连通; 并且第二子室包括出口。

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