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1.发明公开METHODS FOR OVERCOMING GLUTAMINE DEPRIVATION DURING MAMMALIAN CELL CULTURE 审中-公开VERFAHREN ZURÜBERWINDUNGVON GLUTAMINDEPRIVATION BEI EINERSÄUGETIERZELLKULTUR
公开(公告)号：EP3122869A1
公开(公告)日：2017-02-01
申请号：EP15716252.0
申请日：2015-03-24
发明人： YANG, William , HUANG, Yao-Ming , MARTY, Sinacore , RYLL, Thomas , LU, Jiuyi , LEE, Jaesun , LIM, Hyoung Taek , YANG, Eunchong , LEE, Kyngju
IPC分类号： C12N5/00
CPC分类号： C12N5/0018 , C12N2500/40 , C12N2500/60 , C12P21/00
摘要： The present invention pertains to methods of producing polypeptide of interest in cell cultures lacking glutamine. The present invention further pertains to a method of producing a protein of interest in a large scale cell culture, comprising supplementing the cell culture with nucleic acid synthesis precursors and/or corticosteroids.
摘要翻译： 本发明涉及在缺乏谷氨酰胺的细胞培养物中产生感兴趣多肽的方法。 本发明还涉及在大规模细胞培养物中生产感兴趣的蛋白质的方法，其包括用核酸合成前体和/或皮质类固醇补充细胞培养物。
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公开(公告)号：EP3122869B1
公开(公告)日：2019-06-05
申请号：EP15716252.0
申请日：2015-03-24
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3.发明公开USE OF PERFUSION SEED CULTURES TO IMPROVE BIOPHARMACEUTICAL FED-BATCH PRODUCTION CAPACITY AND PRODUCT QUALITY 审中-公开PERFUSIONSSAATKULTUREN用于改进生物制药FED批量生产生产能力和产品质量
公开(公告)号：EP3083933A1
公开(公告)日：2016-10-26
申请号：EP14827357.6
申请日：2014-12-19
申请人： Biogen MA Inc.
发明人： YANG, William C. , LU, Jiuyi , HUANG, Yao-Ming , YUAN, Hang , KSHIRSAGAR, Rashmi , RYLL, Thomas
IPC分类号： C12M1/34
CPC分类号： C12N5/0018 , C07K16/00 , C07K2317/14 , C12N2500/10 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2511/00
摘要： Methods of improving the efficiency of production of a protein product of interest in mammalian cell culture are presented. In particular, the methods result in an increase in the quantity of a protein product produced, or decreases protein product production time in a manufacturing-scale bioreactor cell culture. The disclosed methods comprise: (a) culturing the N-1 bioreactor culture to high viable cell densities; and (b) seeding the production bioreactor culture at high viable cell seeding densities.
摘要翻译： 提高了生产的哺乳动物细胞培养目标蛋白产品的效率的方法介绍。 特别地，该方法导致在产生的蛋白质产物的量的增加，或减少蛋白质产物的生产时间在制造规模的生物反应器中细胞培养物。 盘游离缺失的方法包括：（a）培养的N-1生物反应器培养至高细胞密度可行的; 和（b）在高活细胞接种密度接种生产生物反应器培养。
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4.发明公开METHODS FOR IMPROVED PRODUCTION AND RECOVERY OF RECOMBINANT PROTEINS FROM EUKARYOTIC CELL CULTURES 审中-公开方法从真核细胞培养物提高了生产和提取的重组蛋白
公开(公告)号：EP2943582A1
公开(公告)日：2015-11-18
申请号：EP14738023.2
申请日：2014-01-10
申请人： Biogen MA Inc.
发明人： GILBERT, Alan , YANG, William C. , HUANG, Yao-Ming , LU, Jiuyi
CPC分类号： C12N1/38 , C12N5/0037 , C12N2500/12 , C12N2500/24 , C12N2500/30 , C12N2500/32 , C12N2500/50 , C12N2500/60 , C12N2501/065 , C12N2501/90 , C12N2510/02 , C12P21/02
摘要： The present disclosure is directed to methods to improve the yields of proteins recombinantly produced in eukaryotic cells comprising the addition of anionic substances such as valproate, malate, succinate, fumarate, citrate (e.g., ferric citrate), polyanionic compounds (e.g., polysulfated compounds such dextran sulfate or polyvinyl sulfate), and combinations thereof, wherein the addition of such compounds reduces the binding of recombinant proteins to the cell surface, therefore increasing the amount of soluble protein that can recovered from the culture medium. In addition, the protein yields can be improved according to the disclosed methods by preventing or reducing cell growth inhibition caused by the binding of the expressed recombinant proteins (e.g., neublastin, or antibodies or antigen-binding fragments thereof) to the cell surface. The disclosed anionic substances can also be used during the harvesting phase of a protein production process to release the expressed recombinant proteins from the cell surface prior to protein purification.
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