公开(公告)号：EP3058059A2

公开(公告)日：2016-08-24

申请号：EP14854424.0

申请日：2014-10-17

申请人： Biopetrolia AB

发明人： NIELSEN, Jens ,  SIEWERS, Verena ,  KRIVORUCHKO, Anastasia ,  ZHANG, Yiming ,  ZHOU, Yongjin ,  BUIJS, Nicolaas A. A. ,  DAI, Zongjie

IPC分类号： C12N1/19 ,  C12P7/42

CPC分类号： C12N15/52 ,  C12N9/0008 ,  C12N9/0051 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/13 ,  C12N9/88 ,  C12N9/93 ,  C12P5/00 ,  C12P7/00 ,  C12Y102/04001 ,  C12Y102/07001 ,  C12Y208/01008 ,  C12Y401/01001 ,  C12Y603/01

摘要： The invention relates to engineering of acetyl-CoA metabolism in yeast and in particular to production of acetyl-CoA in a non-ethanol producing yeast lacking endogenous gene(s) encoding pyruvate decarboxylase and comprising a heterologous pathway for synthesis of cytosolic acetyl-CoA.

摘要翻译： 本发明涉及在酵母中乙酰辅酶A代谢的工程，特别是在缺乏编码丙酮酸脱羧酶的内源基因的非乙醇生产酵母中产生乙酰辅酶A，并且包含用于合成细胞溶质乙酰辅酶A的异源途径。

公开(公告)号：EP3277827A1

公开(公告)日：2018-02-07

申请号：EP16717489.5

申请日：2016-04-01

申请人： Biopetrolia AB

发明人： DAVID, Florian ,  SIEWERS, Verena ,  KRIVORUCHKO, Anastasia ,  WENNING, Leonie ,  YU, Tao ,  ZHOU, Yong-Jin ,  NIELSEN, Jens

IPC分类号： C12P7/64

CPC分类号： C12P7/6409 ,  C12N9/0008 ,  C12N9/1029 ,  C12P7/04 ,  C12P7/6463 ,  C12Y102/0105 ,  C12Y203/01075

摘要： The present invention generally relates to a genetically modified fungal cell capable of producing a very long, chain fatty acid (VLCFA) and/or a VLCFA derivative. The genetically modified fungal cell comprises at least one exogenous gene encoding a fatty acyl-CoA reductase, and at least one gene encoding an elongase, and/or at least one gene encoding a fatty acid synthase.

公开(公告)号：EP3268468A1

公开(公告)日：2018-01-17

申请号：EP16714581.2

申请日：2016-03-10

申请人： Biopetrolia AB

发明人： QIN, Jiufu ,  KRIVORUCHKO, Anastasia ,  DAVID, Florian ,  JIANG, Bo ,  NIELSEN, Jens

IPC分类号： C12N9/10 ,  C12N15/52 ,  C12P13/00 ,  C12P13/10

CPC分类号： C12P13/10 ,  C12N9/0008 ,  C12N9/1018 ,  C12N9/1096 ,  C12N9/1217 ,  C12N9/78 ,  C12N9/88 ,  C12N15/52 ,  C12P13/001 ,  C12Y102/01038 ,  C12Y201/03003 ,  C12Y206/01011 ,  C12Y207/02008 ,  C12Y305/03001 ,  C12Y401/01017 ,  C12Y403/02001

摘要： The present invention relates to the provision of genetically modified microbial cells, such as yeast cells with an improved ability for producing L-ornithine and its derivatives. Overproduction of L-ornithine is obtained in the first place by the down-regulation or attenuation of specially selected genes, wherein said genes encode enzymes involved in the L-ornithine consumption and/or degradation pathways. Further L-ornithine production ability is improved by down-regulation, attenuation, deletion or overexpression of specially selected genes, wherein said genes encode enzymes and/or proteins involved in the L-ornithine ‘acetylated derivatives cycle’, L-glutamate synthesis pathways, subcellular trafficking, TCA cycle, pyruvate carboxylation pathway, respiratory electron-transport chain, and the carbon substrates' assimilation machinery. The invention additionally provides a method to produce L-ornithine with said modified eukaryotic cells.