公开(公告)号：EP1266022B1

公开(公告)日：2008-10-22

申请号：EP01920614.3

申请日：2001-03-21

申请人： CELL GENESYS, INC.

发明人： YU, De-Chao ,  LI, Yuanhao ,  HENDERSON, Daniel, R. ,  LITTLE, Andrew S.

IPC分类号： C12N15/861 ,  C12N15/63 ,  C12N5/10 ,  A61K48/00 ,  A61P35/00

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2710/10343 ,  C12N2830/00 ,  C12N2830/001 ,  C12N2830/008 ,  C12N2830/85 ,  C12N2840/206

摘要： Disclosed herein are replication-competent adenovirus vectors comprising co-transcribed first and second genes under transcriptional control of a heterologous, target cell-specific transcriptional regulatory element (TRE), wherein the second gene is under translational control of an internal ribosome entry site. Methods for the preparation and use of such vectors are also provided. The vectors provide target cell-specific virus replication in applications such as cancer therapy and gene therapy.

公开(公告)号：EP1678193B1

公开(公告)日：2008-12-31

申请号：EP04789038.9

申请日：2004-09-24

申请人： CELL GENESYS, INC.

发明人： ARROYO, Trini ,  LI, Yuanhao

IPC分类号： C07H21/04 ,  C12N15/861 ,  A61P35/00 ,  A61K48/00 ,  A61K35/76

CPC分类号： C12N15/86 ,  A61K35/761 ,  A61K48/0058 ,  C07H21/04 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2830/85 ,  C12N2840/203

摘要： Liver cancer, particularly hepatocellular carcinoma specific promoter sequences and adenovirus vehicles are provided. By providing for transcriptional initiating regulation dependent upon transcription factors that are only active in specific, limited cell types, virus replication will be restricted to the target cells. The modified adenovirus may be used as a vehicle for introducing new genetic capability, particularly associated with cytotoxicity, for treating neoplasia.

公开(公告)号：EP1644491A2

公开(公告)日：2006-04-12

申请号：EP04753654.5

申请日：2004-05-28

申请人： CELL GENESYS, INC.

发明人： LI, Yuanhao ,  FARSON, Deborah ,  TAO, Luqun ,  YU, De Chao

IPC分类号： C12N5/10 ,  C12N15/10 ,  C12N15/33 ,  C12N15/34 ,  C12N15/63 ,  C12N15/64 ,  C12N15/861

CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/48 ,  C12N2830/60 ,  C12N2830/90 ,  C12N2840/44

摘要： Adenovirus packaging cell lines for growth of E1A/E1B deficient adenovirus that is substantially free of replication competent adenovirus (RCA), are provided. Methods for producing adenovirus substantially free of RCA are also provided, wherein the adenovirus is grown in a cell line containing coding sequences for adenovirus E1A and E1B, are operably linked to promoters that lack polynucleotide sequences sharing substantial sequence identity with the native adenovirus E1A and E1B promoters.

公开(公告)号：EP1678193A1

公开(公告)日：2006-07-12

申请号：EP04789038.9

申请日：2004-09-24

申请人： CELL GENESYS, INC.

发明人： ARROYO, Trini ,  LI, Yuanhao

IPC分类号： C07H21/04 ,  C12N15/861

CPC分类号： C12N15/86 ,  A61K35/761 ,  A61K48/0058 ,  C07H21/04 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2830/85 ,  C12N2840/203

摘要： Liver cancer, particularly hepatocellular carcinoma specific promoter sequences and adenovirus vehicles are provided. By providing for transcriptional initiating regulation dependent upon transcription factors that are only active in specific, limited cell types, virus replication will be restricted to the target cells. The modified adenovirus may be used as a vehicle for introducing new genetic capability, particularly associated with cytotoxicity, for treating neoplasia.

摘要翻译： 提供了肝癌，特别是肝细胞癌特异性启动子序列和腺病毒载体。 通过提供依赖于仅在特定的有限的细胞类型中有活性的转录因子的转录起始调节，病毒复制将限于靶细胞。 修饰的腺病毒可以用作介导新的遗传能力，特别是与细胞毒性相关的载体，用于治疗瘤形成。

公开(公告)号：EP1628535A2

公开(公告)日：2006-03-01

申请号：EP04754018.2

申请日：2004-06-02

申请人： CELL GENESYS, INC.

发明人： KO, Derek ,  LI, Yuanhao ,  HARDING, Thomas ,  FANG, Jianmin ,  RAMESH, Nagarajan ,  YU, De Chao

IPC分类号： A01N63/00 ,  A61K48/00 ,  C12N5/00 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63 ,  C12N15/64 ,  C12N15/66 ,  C12N15/70 ,  C12N15/74

CPC分类号： C12N15/86 ,  A61K35/761 ,  A61K38/162 ,  A61K38/193 ,  C12N7/00 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2770/32134 ,  C12N2830/008 ,  C12N2830/20 ,  A61K2300/00

摘要： Cell specific replication-competent viral vectors comprising a self processing peptide cleavage sequence are provided. The targeted replication-competent viral vectors include two or more co-transcribed genes under transcriptional control of the same heterologous transcriptional regulatory element (TRE), wherein at least a second gene is under translational control of a self processing cleavage sequence or 2A sequence. Exemplary vector constructs may further include an additional proteolytic cleavage site which provides a means to remove the self processing peptide sequence from the viral vector.

公开(公告)号：EP1266022A2

公开(公告)日：2002-12-18

申请号：EP01920614.3

申请日：2001-03-21

申请人： CELL GENESYS, INC.

发明人： YU, De-Chao ,  LI, Yuanhao ,  HENDERSON, Daniel, R.

IPC分类号： C12N15/861 ,  C12N15/63 ,  C12N5/10 ,  A61K48/00 ,  A61P35/00

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2710/10343 ,  C12N2830/00 ,  C12N2830/001 ,  C12N2830/008 ,  C12N2830/85 ,  C12N2840/206

摘要： Disclosed herein are replication-competent adenovirus vectors comprising co-transcribed first and second genes under transcriptional control of a heterologous, target cell-specific transcriptional regulatory element (TRE), wherein the second gene is under translational control of an internal ribosome entry site. Methods for the preparation and use of such vectors are also provided. The vectors provide target cell-specific virus replication in applications such as cancer therapy and gene therapy.