公开(公告)号：EP3785529A1

公开(公告)日：2021-03-03

申请号：EP19193990.9

申请日：2019-08-28

申请人： Carlsberg A/S

发明人： KNUDSEN, Søren ,  OLSEN, Ole ,  THOMSEN, Hanne Cecilie ,  PEDAS, Pai Rosager ,  WENDT, Toni ,  SKADHAUGE, Birgitte

IPC分类号： A01H5/10 ,  A01H6/46 ,  C12N15/82 ,  C12Q1/6895 ,  C12C1/00

摘要： The present invention relates to barley plants having grains with altered grain protein content due to mutation of the NAC transcription factor gene NAM-1. In particular, the invention relates to barley plants having grains with a grain protein content advantageous for production of barley based beverages, e.g. beer. The invention further relates to methods for production of barley based beverages, as well as to products prepared from the barley plants of the invention.

公开(公告)号：EP0458846A1

公开(公告)日：1991-12-04

申请号：EP90903321.0

申请日：1990-02-16

申请人： CARLSBERG A/S ,  Akademie der Wissenschaften der DDR

发明人： BORRISS, Rainer, Institut für Mikrobiologie ,  HOFEMEISTER, Jürgen; Zentralinstitut für Genetik ,  THOMSEN, Karl, Kristian ,  OLSEN, Ole ,  VON WETTSTEIN, Dietrich

IPC分类号： A01H5 ,  A23K1 ,  C12C1 ,  C12N1 ,  C12N9 ,  C12N15 ,  C12R1

CPC分类号： C12Y302/01006 ,  A23K20/189 ,  C07K2319/00 ,  C07K2319/02 ,  C12N9/244 ,  C12N15/8242

摘要： La présente invention se rapporte à de nouvelles (1,3-1,4)-beta-glucanases hybrides thermostables, à leur utilisation dans la fabrication de produits alimentaires et dans la fabrication de fourrages, à des fragments d'ADN codant pour de telles glucanases, à des organismes exprimant les fragments d'ADN et à un procédé de production des (1,3-1,4)-beta-glucanases thermostables. On a construit des gènes de fusion hybrides codant pour les (1,3-1,4)-beta-glucanases de Bacillus, dont les produits géniques sont plus thermostables que n'importe quelle (1,3-1,4)-beta-glucanase connue jusqu'ici. Ces gènes hybrides ont été construits par échanges réciproques des parties de terminaison amino et de terminaison carboxy des gènes codant pour la beta-glucanase, qui proviennent du Bacillus amyloliquefaciens et du Bacillus macerans. Les (1,3-1,4)-beta-glucanases thermostables qui en résultent conservent une activité enzymatique notable à des températures dépassant 65°C et à des valeurs de pH inférieures à 5,0.

公开(公告)号：EP0458846B1

公开(公告)日：1996-10-02

申请号：EP90903321.9

申请日：1990-02-16

申请人： CARLSBERG A/S ,  Akademie der Wissenschaften der DDR

发明人： BORRISS, Rainer,Institut für Mikrobiologie ,  HOFEMEISTER, Jürgen; Zentralinstitut für Genetik ,  THOMSEN, Karl, Kristian ,  OLSEN, Ole ,  VON WETTSTEIN, Dietrich

IPC分类号： C12N9/42 ,  C12N15/56

CPC分类号： C12Y302/01006 ,  A23K20/189 ,  C07K2319/00 ,  C07K2319/02 ,  C12N9/244 ,  C12N15/8242

摘要： Novel hybrid thermostable (1,3-1,4)-β-glucanases, their use in food manufacturing and feed manufacturing, DNA fragments encoding such glucanases, organisms expressing the DNA fragments and a method for producing the thermostable (1,3-1,4)-β-glucanases. Hybrid fusion genes encoding Bacillus (1,3-1,4)-β-glucanases were constructed, the gene products of which are more thermostable than any (1,3-1,4)-β-glucanase known until now. The hybrid genes were constructed by reciprocal exchanges of the amino-terminal and carboxy-terminal parts of the β-glucanase encoding genes from Bacillus amyloliquefaciens and Bacillus macerans. The resulting thermostable (1,3-1,4)-β-glucanases retain a significant enzymatic activity at temperatures exceeding 65°C and at pH values below 5.0.

公开(公告)号：EP4114170A1

公开(公告)日：2023-01-11

申请号：EP21708005.0

申请日：2021-03-01

申请人： Carlsberg A/S

发明人： OLSEN, Ole ,  LOK, Finn ,  KNUDSEN, Søren ,  MARRI, Lucia ,  STRIEBECK, Alexander ,  PEDAS, Pai, Rosager ,  CUESTA-SEIJO, Jose, Antonio ,  THOMSEN, Hanne ,  BRAUNE, Katarzyna, Birch

IPC分类号： A01H5/10 ,  A01H6/46 ,  C07K14/415 ,  A01H1/00 ,  C12N15/82

公开(公告)号：EP3731628A1

公开(公告)日：2020-11-04

申请号：EP18826074.9

申请日：2018-12-21

申请人： Carlsberg A/S

发明人： DOCKTER, Christoph ,  PEDAS, Pai, Rosager ,  KNUDSEN, Søren ,  OLSEN, Ole ,  MARRI, Lucia ,  KRUCEWICZ, Katarzyna ,  LOK, Finn ,  WENDT, Toni ,  CARCIOFI, Massimiliano ,  THOMSEN, Hanne ,  RASMUSSEN, Magnus

IPC分类号： A01H5/10 ,  A01H6/46

公开(公告)号：EP3731627A1

公开(公告)日：2020-11-04

申请号：EP18826073.1

申请日：2018-12-21

申请人： Carlsberg A/S

发明人： KNUDSEN, Søren ,  BODEVIN, Sabrina ,  OLSEN, Ole ,  THOMSEN, Hanne ,  WENDT, Toni ,  HARHOLT, Jesper ,  LOK, Finn

IPC分类号： A01H5/10 ,  C07K14/415 ,  C12N9/10 ,  C12N15/82

公开(公告)号：EP1727905A2

公开(公告)日：2006-12-06

申请号：EP05706820.7

申请日：2005-03-09

申请人： Carlsberg A/S

发明人： BREDDAM, Klaus ,  OLSEN, Ole ,  SKADHAUGE, Birgitte ,  LOK, Finn ,  KNUDSEN, Soren ,  BECH, Lene, Molskov

IPC分类号： C12N15/82

CPC分类号： C12C1/18 ,  A01H5/10 ,  C12C5/004 ,  C12C7/04 ,  C12C7/047 ,  C12C11/003 ,  C12C12/00 ,  C12N9/0069 ,  C12N15/8242 ,  C12N15/8243

摘要： According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, which-through further enzymatic or spontaneous reactions-may lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off flavors, even after prolonged storage of the beverage.

摘要翻译： 根据本发明，提供了无效LOX-1大麦及其生产的植物产品，例如通过使用缺乏合成脂肪酸转化酶脂氧合酶-1的大麦籽粒制备的麦芽。 所述酶涉及与亚油酸转化为9-氢过氧十八碳二烯酸（脂氧合酶途径代谢物）相关的主要活性，其通过进一步的酶促或自发反应可能导致反式-2-壬烯醛的出现。 本发明使酿造商能够生产没有可检测的反-2-非特异性特异性异味的啤酒，即使在饮料长期储存之后。

公开(公告)号：EP3732279A1

公开(公告)日：2020-11-04

申请号：EP18830274.9

申请日：2018-12-21

申请人： Carlsberg A/S

发明人： LOK, Finn ,  KRUCEWICZ, Katarzyna ,  MARRI, Lucia ,  SKADHAUGE, Birgitte ,  KNUDSEN, Søren ,  WENDT, Toni ,  OLSEN, Ole

IPC分类号： C12C1/027 ,  C12C1/047 ,  C12C1/125 ,  C12C1/18 ,  C12C7/00 ,  C12C7/01 ,  C12C7/04 ,  A23L2/38

公开(公告)号：EP3792357A1

公开(公告)日：2021-03-17

申请号：EP20200115.2

申请日：2017-06-23

申请人： Carlsberg A/S

发明人： OLSEN, Ole ,  SKADHAUGE, Birgitte ,  KNUDSEN, Søren ,  WENDT, Toni ,  THOMSEN, Hanne, Cecilie ,  STRIEBECK, Alexander ,  WOHLFAHRT, Rasmussen, Magnus ,  CARCIOFI, Massimiliano

IPC分类号： C12N15/01 ,  C12N15/10 ,  C12N15/85

摘要： In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR -based systems and a set-up to propagate and analyze identified, mutated organisms.

公开(公告)号：EP3478832A1

公开(公告)日：2019-05-08

申请号：EP17733800.1

申请日：2017-06-23

申请人： Carlsberg A/S

发明人： WENDT, Toni ,  OLSEN, Ole ,  KNUDSEN, Søren ,  THOMSEN, Hanne, Cecilie ,  STRIEBECK, Alexander ,  SKADHAUGE, Birgitte ,  RASMUSSEN, Magnus Wohlfahrt ,  CARCIOFI, Massimiliano

IPC分类号： C12N15/01 ,  C12N15/10 ,  C12N15/85