摘要:
The present invention relates to a method for the determination of a nucleic acid sequence by physical manipulation. The method is based on the precise determination of the localization of the replicating fork on the template by measuring the physical distance between one end of the molecule and the fork. This allows the determination of the physical location of the site where a pause or a blockage of the replication occurs, and deducing therefrom information on the sequence of the nucleic acid.
摘要:
The present invention relates to a method for determining whether a protein binds to a specific DNA sequence. This method is useful in particular for identifying modifications to the DNA sequence (e.g. methylations) via the binding of proteins that specifically recognize those modifications (e.g. antibodies), but also to identify the binding sequence on DNA of a variety of proteins.
摘要:
The present invention relates to a method for determining whether a protein binds to a specific DNA sequence. This method is useful in particular for identifying modifications to the DNA sequence (e.g. methylations) via the binding of proteins that specifically recognize those modifications (e.g. antibodies), but also to identify the binding sequence on DNA of a variety of proteins.
摘要:
The present invention relates to a fast method for the detection and the quantification of a nucleic acid, DNA or RNA. Specifically, the invention provides a method for detecting and quantifying the presence of a specific nucleic acid molecule which is based on physical and electronic treatments. The method of the invention is particularly useful for applications as diverse as detection of chromosomal abnormal distributions or gene expression analysis.
摘要:
The invention relates to an optical device (1) for measuring the position of an object (B) along a first axis (z), the object (B) being subjected to light radiations emitted by a light source (2, 21, 22), the optical device (1) comprising: - an imaging system (5) comprising an objective for collecting light radiations diffused by the object (B), the imaging system (5) having an optical axis (O) extending parallel to the first axis (z), - a transmission mask (8) having at least a first pair of apertures (81, 83) and a second pair of apertures (82, 84), the first pair of apertures and second pair of apertures being spaced from each other along a second axis (x), perpendicular to the first axis (z), the transmission mask dividing the radiation diffused by the object (B) into two first beams passing through the first pair of apertures (81, 83) and two second beams passing through the second pair of apertures (82, 84), while blocking a part of the radiations (R3) emitted by the light source which is not diffused by the object, - a detector (6) adapted for generating an image including a first spot (S1) and a second spot (S2) representative of the first part (R1) and second part (R2) of the radiations impacting the detector plane (61), wherein variation of the position of the object (B) relative to the object plane of the imaging system (5) along the first axis (z) spatial phase shifting of first interference patterns and of the second interference pattern relative to each other.
摘要:
The invention relates to an optical device (1) for measuring the position of an object (B) along a first axis (z), the object (B) being subjected to light radiations emitted by a light source (2, 21, 22), the optical device (1) comprising: - an imaging system (5) comprising an objective for collecting light radiations diffused by the object (B), the imaging system (5) having an optical axis (O) extending parallel to the first axis (z), - a transmission mask (8) having at least a first aperture (81, 83) and a second aperture (82, 84), the first aperture and second aperture being spaced from each other along a second axis (x), perpendicular to the first axis (z), the transmission mask (8) being arranged so as to let a first part of the radiations (R1) and a second part of the radiations (R2) which are diffused by the object (B) pass through the first aperture and the second aperture respectively, while blocking a part of the radiations (R3) emitted by the light source which is not diffused by the object, and - a detector (6) adapted for generating an image including a first spot (S1) and a second spot (S2) representative of the first part (R1) and second part (R2) of the radiations impacting the detector plane (61), wherein variation of the position of the object (B) relative to the object plane of the imaging system (5) along the first axis (z) causes variation of a position of the first spot (S1) and of the second spot (S2) relative to each other along the second axis (x).
摘要:
The invention relates to an optical device (1) for measuring the position of an object (B) along a first axis (z), the object (B) being subjected to light radiations emitted by a light source (2, 21, 22), the optical device (1) comprising: - an imaging system (5) comprising an objective for collecting light radiations diffused by the object (B), the imaging system (5) having an optical axis (O) extending parallel to the first axis (z), - a transmission mask (8) having at least a first aperture (81, 83) and a second aperture (82, 84), the first aperture and second aperture being spaced from each other along a second axis (x), perpendicular to the first axis (z), the transmission mask (8) being arranged so as to let a first part of the radiations (R1) and a second part of the radiations (R2) which are diffused by the object (B) pass through the first aperture and the second aperture respectively, while blocking a part of the radiations (R3) emitted by the light source which is not diffused by the object, and - a detector (6) adapted for generating an image including a first spot (S1) and a second spot (S2) representative of the first part (R1) and second part (R2) of the radiations impacting the detector plane (61), wherein variation of the position of the object (B) relative to the object plane of the imaging system (5) along the first axis (z) causes variation of a position of the first spot (S1) and of the second spot (S2) relative to each other along the second axis (x).
摘要:
The present invention relates to a fast method for the detection and the quantification of a nucleic acid, DNA or RNA. Specifically, the invention provides a method for detecting and quantifying the presence of a specific nucleic acid molecule which is based on physical and electronic treatments. The method of the invention is particularly useful for applications as diverse as detection of chromosomal abnormal distributions or gene expression analysis.
摘要:
The present invention relates to a method for the determination of a nucleic acid sequence by physical manipulation. The method is based on the precise determination of the localization of the replicating fork on the template by measuring the physical distance between one end of the molecule and the fork. This allows the determination of the physical location of the site where a pause or a blockage of the replication occurs, and deducing therefrom information on the sequence of the nucleic acid.