公开(公告)号：EP3190123A1

公开(公告)日：2017-07-12

申请号：EP16201269.4

申请日：2010-09-24

申请人： Codexis, Inc.

发明人： COLLIER, Steven James ,  TEO, Ee Ling ,  SUKUMARAN, Joly ,  WILSON, Robert John ,  XU, Junye

IPC分类号： C07K14/00 ,  C12N9/10 ,  C12P7/00

CPC分类号： C12P17/06 ,  C07K14/00 ,  C12N9/1025 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/62 ,  C12Y203/01 ,  Y02P20/52

摘要： Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.

摘要翻译： 提出了使用LovD酰基转移酶，具有酰基的硫酯和（i）硫醇清除剂和/或（ii）沉淀剂改善化学底物酰化的方法。 还提出了使用（i）活性炭作为硫醇清除剂和/或（ii）氢氧化铵作为沉淀剂生产辛伐他汀的改进方法。

公开(公告)号：EP3190123B1

公开(公告)日：2019-07-24

申请号：EP16201269.4

申请日：2010-09-24

申请人： Codexis, Inc.

发明人： COLLIER, Steven James ,  TEO, Ee Ling ,  SUKUMARAN, Joly ,  WILSON, Robert John ,  XU, Junye

IPC分类号： C07K14/00 ,  C12N9/10 ,  C12P7/00

公开(公告)号：EP2483292B1

公开(公告)日：2014-11-12

申请号：EP10821065.9

申请日：2010-09-24

申请人： Codexis, Inc.

发明人： GILSON, Lynne ,  COLLIER, Steven James ,  SUKUMARAN, Joly ,  YEO, Wan Lin ,  ALVISO, Oscar ,  TEO, Ee Ling ,  WILSON, Robert John ,  XU, Junye

IPC分类号： C07K14/00 ,  C12N9/10 ,  C12P7/62 ,  C12P17/06

CPC分类号： C12P17/06 ,  C07K14/00 ,  C12N9/1025 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/62 ,  C12Y203/01 ,  Y02P20/52

摘要： Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.

摘要翻译： 本公开提供了可用于合成治疗重要的他汀类化合物的酰基转移酶。

公开(公告)号：EP2483292A1

公开(公告)日：2012-08-08

申请号：EP10821065.9

申请日：2010-09-24

申请人： Codexis, Inc.

发明人： GILSON, Lynne ,  COLLIER, Steven James ,  SUKUMARAN, Joly ,  YEO, Wan Lin ,  ALVISO, Oscar ,  TEO, Ee Ling ,  WILSON, Robert John ,  XU, Junye

IPC分类号： C07K14/00

CPC分类号： C12P17/06 ,  C07K14/00 ,  C12N9/1025 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/62 ,  C12Y203/01 ,  Y02P20/52

摘要： Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.