公开(公告)号：EP1273665A4

公开(公告)日：2006-04-05

申请号：EP01908255

申请日：2001-03-02

申请人： DAIICHI FINE CHEM CO LTD

发明人： SAKAMOTO KEIJI ,  KITA SHINJI ,  TSUZAKI KAZUYA ,  MORIKAWA TADANORI ,  SHIMIZU SAKAYU ,  KATAOKA MICHIHIKO

IPC分类号： C12P13/00 ,  C12P41/00 ,  C12R1/01 ,  C12R1/22 ,  C12R1/32 ,  C12R1/40 ,  C12R1/43 ,  C12R1/64 ,  C12R1/645

CPC分类号： C12P13/001 ,  C12P13/008 ,  C12P41/002 ,  Y10S435/822 ,  Y10S435/865 ,  Y10S435/877 ,  Y10S435/911 ,  Y10S435/914 ,  Y10S435/917 ,  Y10S435/918

摘要： A process for producing an optical active beta -amino alcohol, the method comprising the step of allowing at least one microorganism selected from the group consisting of microorganisms belonging to the genus Morganella and others, to act on an enantiomeric mixture of an alpha -aminoketone or a salt thereof having the general formula (I): to produce an optical active beta -amino alcohol with the desired optical activity having the general formula (II) described below in a high yield as well as in a highly selective manner:

公开(公告)号：EP1600499A4

公开(公告)日：2006-08-30

申请号：EP04716788

申请日：2004-03-03

申请人： DAIICHI FINE CHEM CO LTD

发明人： SHIMIZU SAKAYU ,  KATAOKA MICHIHIKO ,  SAKAMOTO KEIJI

IPC分类号： C12N1/15 ,  C12N9/18 ,  C12N15/55 ,  C12P17/04 ,  C12P41/00

CPC分类号： C12P41/003 ,  C12N9/18 ,  C12P17/04

摘要： It has been required to economically produce a large amount of an optically active gamma-lactone derivative (for example, pantolactone) which is useful as an intermediate in synthesizing a useful substance such as a drug. To achieve this object, it is advantageous to employ a technique of enzymatic asymmetric hydrolysis with the use of an enzyme lactonase. However, it remains troublesome to prepare the enzyme or utilize a microorganism capable of producing the enzyme. Also, it is difficult to obtain a sufficient and stable enzymatic activity in the case of using recombination techniques. In producing lactonase, which asymmetrically hydrolyzes a gamma-lactone derivative such as racemic pantolactone selectively, by a recombination technique, maturation lactonase DNA and signal peptide region DNA are both transferred into a host. Thus, a stable activity comparable to that of natural lactonase can be achieved and a transformant having a high enzyme activity and sustaining it in a stable state can be obtained. As a result, it becomes possible to efficiently and industrially advantageously perform the asymmetric synthesis of a gamma-lactone derivative.