公开(公告)号：EP1640025A4

公开(公告)日：2009-03-25

申请号：EP04746481

申请日：2004-06-25

申请人： SUNTORY LTD

发明人： MAEDA MITSURU ,  NAKAO MASAHIRO ,  FUKAMI HARUKAZU

IPC分类号： A61L31/00 ,  A61K8/67 ,  A61K8/99 ,  A61K31/704 ,  A61Q19/00 ,  A61Q19/02

CPC分类号： A61Q19/02 ,  A61K8/676 ,  A61K8/99 ,  A61K31/704 ,  A61K2800/522 ,  A61K2800/70 ,  A61K2800/75 ,  A61K2800/782 ,  A61Q19/00 ,  Y10S435/913 ,  Y10S435/914 ,  Y10S435/918

摘要： It is intended to provide a skin composition for external use that contains an ascorbic acid derivative having a high stability, being continuously usable in vivo, having a strong antioxidant effect and showing little skin irritation and has an excellent skin permeability. A composition for external use characterized by containing 2-O-(β-D-glucopyranosyl)ascorbic acid represented by the formula (I) or its salt or ester being safe to the human body together with optionally treated koji mold cells.

公开(公告)号：EP0654095A4

公开(公告)日：1999-04-07

申请号：EP93918612

申请日：1993-08-03

申请人： US BIOCHEMICAL CORP

发明人： FULLER CARL W

IPC分类号： B01D57/02 ,  C12N15/09 ,  C12N15/10 ,  C12Q1/68 ,  G01N27/447 ,  C09K3/00 ,  C12P19/34 ,  G01N33/00

CPC分类号： B01D57/02 ,  C12N15/101 ,  C12Q1/6869 ,  G01N27/44726 ,  G01N27/44747 ,  Y10S435/914 ,  Y10S435/915 ,  Y10T436/143333

摘要： A method for electrophoresis of nucleic acid fragments present in the solution which contains an amount, e.g., 0.2 % or more, of a reagent, e.g., glycerol, dithiolthreitol (DTT) and trehalose or other sugars, which interact to form a complex with borate or boric acid. The method includes applying the solution to an electrophoretic gel and electrophoresing those fragments into the gel in the presence of a buffer lacking boric acid, or a derivative thereof, which forms a chelate complex with the reagent and thereby causes distortion of electrophoresis of the fragments in a gel including such a buffer.

公开(公告)号：EP1640025B1

公开(公告)日：2010-12-15

申请号：EP04746481.3

申请日：2004-06-25

申请人： Suntory Holdings Limited

发明人： MAEDA, Mitsuru ,  NAKAO, Masahiro ,  FUKAMI, Harukazu

IPC分类号： A61L31/00

CPC分类号： A61Q19/02 ,  A61K8/676 ,  A61K8/99 ,  A61K31/704 ,  A61K2800/522 ,  A61K2800/70 ,  A61K2800/75 ,  A61K2800/782 ,  A61Q19/00 ,  Y10S435/913 ,  Y10S435/914 ,  Y10S435/918

摘要： It is intended to provide a skin composition for external use that contains an ascorbic acid derivative having a high stability, being continuously usable in vivo, having a strong antioxidant effect and showing little skin irritation and has an excellent skin permeability. A composition for external use characterized by containing 2-O-(β-D-glucopyranosyl)ascorbic acid represented by the formula (I) or its salt or ester being safe to the human body together with optionally treated koji mold cells.

摘要翻译： 一种外用组合物，其包含由式（I）表示的2-O-（2-D-吡喃葡萄糖基）抗坏血酸：或其对人体安全的盐或酯，以及曲霉或加工曲 。 外用组合物的皮肤透过性优异，含有稳定性优异，抗生素活性强，抗氧化活性强的抗坏血酸衍生物。

公开(公告)号：EP0860500B1

公开(公告)日：2004-09-01

申请号：EP98301183.4

申请日：1998-02-18

申请人： CERESTAR HOLDING B.V.

发明人： Vercauteren, Ronny ,  Dendooven, Els ,  Heylen, An Amanda Jules

IPC分类号： C12N9/28 ,  C12N9/30 ,  C12P19/14 ,  C13K7/00

CPC分类号： C12N9/2417 ,  C12N9/242 ,  C12P19/14 ,  C13K7/00 ,  Y10S435/914 ,  Y10S435/917

公开(公告)号：EP1273665A1

公开(公告)日：2003-01-08

申请号：EP01908255.1

申请日：2001-03-02

申请人： Daiichi Fine Chemical Co., Ltd.

发明人： SAKAMOTO, Keiji ,  KITA, Shinji ,  TSUZAKI, Kazuya ,  MORIKAWA, Tadanori ,  SHIMIZU, Sakayu ,  KATAOKA, Michihiko

IPC分类号： C12P13/00

CPC分类号： C12P13/001 ,  C12P13/008 ,  C12P41/002 ,  Y10S435/822 ,  Y10S435/865 ,  Y10S435/877 ,  Y10S435/911 ,  Y10S435/914 ,  Y10S435/917 ,  Y10S435/918

摘要： A process for producing an optical active β-amino alcohol, the method comprising the step of allowing at least one microorganism selected from the group consisting of microorganisms belonging to the genus Morganella and others, to act on an enantiomeric mixture of an α-aminoketone or a salt thereof having the general formula (I):
to produce an optical active β-amino alcohol with the desired optical activity having the general formula (II) described below in a high yield as well as in a highly selective manner:

摘要翻译： 一种制备光学活性β-氨基醇的方法，所述方法包括允许至少一种选自属于摩根氏菌属的微生物的微生物和其它微生物作用于α-氨基酮或其对映异构体混合物 其具有通式（I）的盐：以高产率和高度选择性的方式制备具有下述通式（II）的所需光学活性的光学活性β-氨基醇：

公开(公告)号：EP0654095A1

公开(公告)日：1995-05-24

申请号：EP93918612.0

申请日：1993-08-03

申请人： UNITED STATES BIOCHEMICAL CORPORATION

发明人： FULLER, Carl, W.

IPC分类号： B01D57 ,  C12N15 ,  C12Q1 ,  G01N27

CPC分类号： B01D57/02 ,  C12N15/101 ,  C12Q1/6869 ,  G01N27/44726 ,  G01N27/44747 ,  Y10S435/914 ,  Y10S435/915 ,  Y10T436/143333

摘要： A method for electrophoresis of nucleic acid fragments present in the solution which contains an amount, e.g., 0.2 % or more, of a reagent, e.g., glycerol, dithiolthreitol (DTT) and trehalose or other sugars, which interact to form a complex with borate or boric acid. The method includes applying the solution to an electrophoretic gel and electrophoresing those fragments into the gel in the presence of a buffer lacking boric acid, or a derivative thereof, which forms a chelate complex with the reagent and thereby causes distortion of electrophoresis of the fragments in a gel including such a buffer.

摘要翻译： 用于电泳存在于溶液中的核酸片段的方法，所述核酸片段含有一定量例如0.2％或更多的试剂例如甘油，二硫苏糖醇（DTT）和海藻糖或其他糖，其与硼酸盐形成复合物 或硼酸。 该方法包括将溶液应用于电泳凝胶，并在缺乏硼酸的缓冲液或其衍生物存在下将这些片段电泳至凝胶中，所述缓冲液与试剂形成螯合络合物，从而引起片段电泳失真 包含这种缓冲液的凝胶。

公开(公告)号：EP1273665B1

公开(公告)日：2007-05-16

申请号：EP01908255.1

申请日：2001-03-02

申请人： Daiichi Fine Chemical Co., Ltd.

发明人： SAKAMOTO, Keiji ,  KITA, Shinji ,  TSUZAKI, Kazuya ,  MORIKAWA, Tadanori ,  SHIMIZU, Sakayu ,  KATAOKA, Michihiko

IPC分类号： C12P13/00 ,  C12R1/01 ,  C12R1/22 ,  C12R1/32 ,  C12R1/40 ,  C12R1/43 ,  C12R1/64 ,  C12R1/645

CPC分类号： C12P13/001 ,  C12P13/008 ,  C12P41/002 ,  Y10S435/822 ,  Y10S435/865 ,  Y10S435/877 ,  Y10S435/911 ,  Y10S435/914 ,  Y10S435/917 ,  Y10S435/918

摘要： A process for producing an optical active beta -amino alcohol, the method comprising the step of allowing at least one microorganism selected from the group consisting of microorganisms belonging to the genus Morganella and others, to act on an enantiomeric mixture of an alpha -aminoketone or a salt thereof having the general formula (I): to produce an optical active beta -amino alcohol with the desired optical activity having the general formula (II) described below in a high yield as well as in a highly selective manner:

公开(公告)号：EP1273665A4

公开(公告)日：2006-04-05

申请号：EP01908255

申请日：2001-03-02

申请人： DAIICHI FINE CHEM CO LTD

发明人： SAKAMOTO KEIJI ,  KITA SHINJI ,  TSUZAKI KAZUYA ,  MORIKAWA TADANORI ,  SHIMIZU SAKAYU ,  KATAOKA MICHIHIKO

IPC分类号： C12P13/00 ,  C12P41/00 ,  C12R1/01 ,  C12R1/22 ,  C12R1/32 ,  C12R1/40 ,  C12R1/43 ,  C12R1/64 ,  C12R1/645

CPC分类号： C12P13/001 ,  C12P13/008 ,  C12P41/002 ,  Y10S435/822 ,  Y10S435/865 ,  Y10S435/877 ,  Y10S435/911 ,  Y10S435/914 ,  Y10S435/917 ,  Y10S435/918

摘要： A process for producing an optical active beta -amino alcohol, the method comprising the step of allowing at least one microorganism selected from the group consisting of microorganisms belonging to the genus Morganella and others, to act on an enantiomeric mixture of an alpha -aminoketone or a salt thereof having the general formula (I): to produce an optical active beta -amino alcohol with the desired optical activity having the general formula (II) described below in a high yield as well as in a highly selective manner:

公开(公告)号：EP1640025A1

公开(公告)日：2006-03-29

申请号：EP04746481.3

申请日：2004-06-25

申请人： SUNTORY LIMITED

发明人： MAEDA, Mitsuru ,  NAKAO, Masahiro ,  FUKAMI, Harukazu

IPC分类号： A61L31/00 ,  A61K8/40 ,  A61K8/41

CPC分类号： A61Q19/02 ,  A61K8/676 ,  A61K8/99 ,  A61K31/704 ,  A61K2800/522 ,  A61K2800/70 ,  A61K2800/75 ,  A61K2800/782 ,  A61Q19/00 ,  Y10S435/913 ,  Y10S435/914 ,  Y10S435/918

摘要： A composition for external use, comprising 2-O-(β-D-glucopyranosyl)ascorbic acid represented by the formula (I):

or a salt or ester thereof which is safe to the human body, and a koji mold or a processed koji. The composition for external use is excellent in skin permeability, containing an ascorbic acid derivative which is excellent in stability, utilized persistently in the living body, and strong in antioxidant activity, and has little skin irritation.

摘要翻译： 一种外用组合物，其包含由式（I）表示的2-O-（2-D-吡喃葡萄糖基）抗坏血酸：或其对人体安全的盐或酯，以及曲霉或加工曲 。 外用组合物的皮肤透过性优异，含有稳定性优异，抗生素活性强，抗氧化活性强的抗坏血酸衍生物。

公开(公告)号：EP0860500A1

公开(公告)日：1998-08-26

申请号：EP98301183.4

申请日：1998-02-18

申请人： CERESTAR HOLDING B.V.

发明人： Vercauteren, Ronny ,  Dendooven, Els ,  Heylen, An Amanda Jules

IPC分类号： C12N9/28 ,  C12N9/30 ,  C12P19/14 ,  C13K7/00

CPC分类号： C12N9/2417 ,  C12N9/242 ,  C12P19/14 ,  C13K7/00 ,  Y10S435/914 ,  Y10S435/917

摘要： The present invention describes a simple and inexpensive purification method for acid-stable fungal alpha-amylase wherein the alpha-amylase is obtained without significant loss of enzyme activity. The purified acid-stable alpha-amylase which is obtained is substantially free from glucoamylase. The method for obtaining an acid-stable alpha-amylase comprises the steps of

adjusting the pH of the enzyme containing solution to a value between 1 and 8,
heating the solution to a temperature and for a time sufficient to inactivate glucoamylase,
removing the denatured glucoamylase.
The purified acid-stable alpha-amylase is used for the conversion of starch. The purified alpha-amylase is also used in an immobilized form.

摘要翻译： 本发明描述了一种简单且廉价的酸稳定型真菌α-淀粉酶纯化方法，其中获得的α-淀粉酶没有显着的酶活性损失。 获得的纯化的酸稳定的α-淀粉酶基本上不含葡糖淀粉酶。 获得酸稳定性α-淀粉酶的方法包括以下步骤：将含酶溶液的pH调节至1至8之间的值，将溶液加热到足以灭活葡糖淀粉酶的温度和时间，除去变性葡糖淀粉酶 。 纯化的酸稳定的α-淀粉酶用于淀粉的转化。 纯化的α-淀粉酶也以固定化形式使用。