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公开(公告)号:EP3309260A1
公开(公告)日:2018-04-18
申请号:EP16194038.2
申请日:2016-10-14
摘要: The invention relates to archaeal pyrrolysyl tRNA synthetases lacking a nuclear localization signal and/or comprising a nuclear export signal. The invention also relates to polynucleotides encoding said pyrrolysyl tRNA synthetases, eukaryotic cells comprising said polynucleotide and tRNA acylated by the pyrrolysyl tRNA synthetase or a polynucleotide encoding such tRNA, methods utilizing said cells for preparing polypeptides comprising unnatural amino acid residues, and kits useful in said methods.
摘要翻译: 本发明涉及缺乏核定位信号和/或包含核输出信号的古细菌吡咯赖氨酰tRNA合成酶。 本发明还涉及编码所述吡咯赖氨酰tRNA合成酶的多核苷酸,包含所述多核苷酸和由吡咯赖氨酰tRNA合成酶酰化的tRNA或编码此类tRNA的多核苷酸的真核细胞,利用所述细胞制备包含非天然氨基酸残基的多肽的方法,以及可用于所述 方法。
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公开(公告)号:EP3526338A1
公开(公告)日:2019-08-21
申请号:EP17791314.2
申请日:2017-10-13
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3.
公开(公告)号:EP3924365A1
公开(公告)日:2021-12-22
申请号:EP20703782.1
申请日:2020-02-14
IPC分类号: C07K14/435 , C12N9/00
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4.
公开(公告)号:EP3384021A1
公开(公告)日:2018-10-10
申请号:EP16805063.1
申请日:2016-11-29
CPC分类号: C12N9/93 , C07K14/47 , C07K16/18 , C07K16/248 , C07K16/32 , C07K2317/55 , C12N5/0601 , C12N9/00 , C12N15/09 , C12N15/52 , C12N15/63 , C12N15/67 , C12N15/86 , C12N2510/02 , C12N2710/14143 , C12P13/005 , C12P21/02 , C12Y601/01026
摘要: The invention relates to a method of preparing engineered target polypeptides (TP) comprising in its amino acid sequence one or more, identical or different, non-canonical amino acid (ncAA) residues, by expressing said TP in an insect cell line (ICL) and by expressing novel orthogonal bacterial aminoacyl tRNA synthetase/tRNA pairs in said ICL; a baculoviral shuttle vector (bacmid) suitable or introducing the genetic information of said orthogonal tRNA synthetase/tRNA into said ILC; particular expression cassettes for expressing said particular tRNAs in said ILC; TPs obtained by said method; as we as a kit for preparing said TPs.
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