ARCHAEAL PYRROLYSYL TRNA SYNTHETASES FOR ORTHOGONAL USE
    1.
    发明公开
    ARCHAEAL PYRROLYSYL TRNA SYNTHETASES FOR ORTHOGONAL USE 审中-公开
    用于正交使用的原虫聚吡咯酰化合物

    公开(公告)号:EP3309260A1

    公开(公告)日:2018-04-18

    申请号:EP16194038.2

    申请日:2016-10-14

    IPC分类号: C12P21/02 C12N15/11

    CPC分类号: C12N15/11 C12P21/02

    摘要: The invention relates to archaeal pyrrolysyl tRNA synthetases lacking a nuclear localization signal and/or comprising a nuclear export signal. The invention also relates to polynucleotides encoding said pyrrolysyl tRNA synthetases, eukaryotic cells comprising said polynucleotide and tRNA acylated by the pyrrolysyl tRNA synthetase or a polynucleotide encoding such tRNA, methods utilizing said cells for preparing polypeptides comprising unnatural amino acid residues, and kits useful in said methods.

    摘要翻译: 本发明涉及缺乏核定位信号和/或包含核输出信号的古细菌吡咯赖氨酰tRNA合成酶。 本发明还涉及编码所述吡咯赖氨酰tRNA合成酶的多核苷酸,包含所述多核苷酸和由吡咯赖氨酰tRNA合成酶酰化的tRNA或编码此类tRNA的多核苷酸的真核细胞,利用所述细胞制备包含非天然氨基酸残基的多肽的方法,以及可用于所述 方法。