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    COMPOSITIONS AND METHODS FOR ALTERING RNAI
    5.
    发明公开
    COMPOSITIONS AND METHODS FOR ALTERING RNAI 审中-公开
    组合物和方法中应用RNA干扰

    公开(公告)号：EP1976566A2

    公开(公告)日：2008-10-08

    申请号：EP06839414.7

    申请日：2006-12-19

    申请人： Northwestern University

    发明人： CARTHEW, Richard ,  LEE, Young, Sik ,  HARRIS, Dinari

    IPC分类号： A61K48/00

    CPC分类号： C12N5/0601 ,  C07K14/43581 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2320/12 ,  C12N2320/50 ,  G01N33/5023 ,  G01N33/5041

    摘要： The present invention relates to compositions and methods for altering (e.g., enhancing) RNAi. In particular, the present invention provides systems and methods for identifying regulators of RNAi. For example, the present invention provides RNAi regulators (e.g., HPSl and HPS4) and methods of altering (e.g., inhibiting) these regulators in order to alter (e.g., enhance) RNAi. The present invention also provides methods of identifying inhibitors (e.g., small molecule, nucleic acid (e.g., siRNA), and antibody) of RNAi regulators and methods of using the same (e.g., to enhance RNAi). Compositions and methods of the present invention find use in research (e.g., functional genomics), therapeutic (e.g., drug discovery and delivery) and clinical applications.

    Method of preparing culture support factor from insects
    6.
    发明公开
    Method of preparing culture support factor from insects 审中-公开
    Methode zur Herstellung eines Faktors aus Insekten zurUnterstützungvon Zellkulturen

    公开(公告)号：EP1352954A2

    公开(公告)日：2003-10-15

    申请号：EP03006077.6

    申请日：2003-03-19

    申请人： HOKKAIDO UNIVERSITY

    发明人： Hayakawa, Yoichi

    IPC分类号： C12N5/06 ,  C07K14/435

    CPC分类号： C12N5/0601 ,  C12N2500/82

    摘要： A method of preparing a culture support factor for cells or tissues from an insect, comprising the steps of steam-sterilizing a worm body of a lava or pupa of the insect, and subjecting the steam-sterilized worm body to extraction with an aqueous extraction solution, thereby recovering the culture support factor in the extraction solution.

    摘要翻译： 一种从昆虫制备细胞或组织的培养支持因子的方法，包括以下步骤：对昆虫的熔岩或蛹的蠕虫体进行蒸汽灭菌，并将所述蒸汽灭菌的蠕虫体用萃取水溶液进行提取 从而回收提取溶液中的培养支持因子.1。一种从昆虫制备细胞或组织的培养支持因子的方法，包括以下步骤：对昆虫的熔岩或蛹的蠕虫体进行蒸汽灭菌， 蒸汽消毒的蠕虫体用萃取水溶液萃取，从而回收提取溶液中的培养支持因子。

    Process and medium for culturing ant venom gland cells
    8.
    发明公开
    Process and medium for culturing ant venom gland cells 失效
    Verfahren und Mittel zur Kultivierung vonAmeisengiftdrüsenzellen。

    公开(公告)号：EP0141182A2

    公开(公告)日：1985-05-15

    申请号：EP84110801.2

    申请日：1984-09-11

    申请人： THE OHIO STATE UNIVERSITY RESEARCH FOUNDATION

    发明人： Hink, Walter F., Jr.

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0601 ,  C12N2500/25 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/38 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/39 ,  C12N2501/81 ,  Y10S435/948

    摘要： In vitro culture of the venom gland cells of Pseudomyremex triplarinus is effected by removing venom gland tissue from a larva or pupa of P. triplarinus subjecting the tissue to a protease to separate out the cells therein and culturing the resultant cells in an isotonic salin medium comprising carbohydrate, amino acids guanosine 3',5'-cyclic monophosphate and adenosine 3',5'-cyclic monophosphoric acid, at least part of the medium having been conditioned with Spodoptera frugiperda cells. Such in vitro culture provides a practicable route to volume production of the biologically active materials which are useful fortreating the symptoms of rheumatoid arthritis and which the venom gland cells are known to produce.

    摘要翻译： 蚂蚁假丝酵母细胞的毒液细胞的体外培养是通过从三木素幼虫或蛹中除去毒液腺组织来实现的，所述三倍体对组织进行蛋白酶分离出细胞，并将所得细胞在等渗盐水中培养 含有碳水化合物，氨基酸鸟苷3'，5'-环状单磷酸和腺苷3'，5'-环状单磷酸的培养基，培养基的至少一部分已用草地贪夜蛾（Spodoptera frugiperda）细胞调理。 这种体外培养提供了可用于批量生产可用于治疗类风湿性关节炎症状的生物活性物质的可行途径，并且已知毒液腺细胞产生。