GENE ENCODING CYCLIC LIPOPEPTIDE ACYLASE AND EXPRESSION OF THE SAME
    3.
    发明公开
    GENE ENCODING CYCLIC LIPOPEPTIDE ACYLASE AND EXPRESSION OF THE SAME 审中-公开
    FüRCYCLISCHE LIPOPEPTID ACYLASE KODEIRENDES GEN UND DESSEN EXPRESSION

    公开(公告)号:EP1197557A1

    公开(公告)日:2002-04-17

    申请号:EP00940872.5

    申请日:2000-06-28

    IPC分类号: C12N15/55 C12N1/21 C12N9/14

    CPC分类号: C12N9/14 C12P21/02

    摘要: The present invention relates to determination of the nucleotide sequence of coding region of cyclic lipopeptide acylase, determination of the full-length amino acid sequence of the acylase, an expression vector containing a gene encoding the enzyme, and a method for producing cyclic lipopeptide acylase by expression of the expression vector in a host cell. According to the present invention, the use of the transformant having the same level of activity as does an acylase obtained by culturing a conventional cyclic lipopeptide acylase producing bacteria, which is obtained by genetic engineering has made it possible to shorten the time necessary for culture (time necessary for producing cyclic lipopeptide acylase).

    摘要翻译: 本发明涉及确定环状脂肽酰基转移酶的编码区的核苷酸序列,测定酰基转移酶的全长氨基酸序列,含有编码该酶的基因的表达载体,以及通过以下方法制备环状脂肽酰基转移酶的方法: 表达载体在宿主细胞中的表达。 根据本发明,通过遗传工程获得的具有与通过培养常规的环状脂肽酰基转移酶生产菌获得的酰基转移酶相同的活性水平的转化体的使用使得可以缩短培养所需的时间( 制备环状脂肽酰基转移酶所需的时间)。

    PROCESS FOR PREPARING FR900482 AND COMPOUNDS ANALOGOUS THERETO
    5.
    发明公开
    PROCESS FOR PREPARING FR900482 AND COMPOUNDS ANALOGOUS THERETO 失效
    VERFAHREN ZUR HERSTELLUNG VON FR900482 UND ANALOGE VERBINDUNGEN

    公开(公告)号:EP0943689A1

    公开(公告)日:1999-09-22

    申请号:EP97909655.9

    申请日:1997-10-28

    IPC分类号: C12P17/18 C12N1/14

    CPC分类号: C12P17/188 Y10S435/929

    摘要: A process for preparing compounds represented by general formula (I), wherein R 1 represent hydrogen, a lower alkanoyl group or a lower alkyl group; and R 2 and R 3 represent each hydrogen or a lower alkanoyl group, characterized by reacting a culture of a strain belonging to the genus Fusarium or cells obtained by the filtration of the culture with a compound represented by general formula (II) wherein R 1 , R 2 , and R 3 are each as defined above.

    摘要翻译: 一种制备由通式(I)表示的化合物的方法,其中R 1表示氢,低级烷酰基或低级烷基; R 2和R 3代表每个氢或低级烷酰基,其特征在于使属于镰刀菌属的菌株的培养物或通过过滤培养物得到的细胞与通式(II)表示的化合物反应 )其中R 1,R 2和R 3各自如上所定义。

    CYCLIC LIPOPEPTIDE ACYLASE
    6.
    发明公开
    CYCLIC LIPOPEPTIDE ACYLASE 失效
    ZYCLISCHE LIPOPEPTID ACYLASE

    公开(公告)号:EP0933422A1

    公开(公告)日:1999-08-04

    申请号:EP97926214.4

    申请日:1997-06-11

    IPC分类号: C12N9/80 C12N1/14 C07K5/12

    摘要: The present invention provides a cyclic lipopeptide acylase which may effectively deacylate the acyl side chain of a cyclic lipopeptide compound, specifically FR901379 Substance or its analog thereof shown by the following general formula [I], and a process for production of a cyclic peptide compound which comprises the use of said acylase.
    [wherein R 1 is acyl;

    R 2 is hydroxy or acyloxy;
    R 3 is hydrogen or hydroxy;
    R 4 is hydrogen or hydroxy;
    R 5 is hydrogen or hydroxysulfonyloxy; and
    R 6 is hydrogen or carbamoyl]

    摘要翻译: 本发明提供了一种环状脂肽酰基转移酶,其可以有效地脱酰化环状脂肽化合物的酰基侧链,特别是FR901379由以下通式ⅪI所示的物质或其类似物,以及一种环肽化合物的制备方法, 使用所述酰基转移酶。 其中R 1是酰基; R 2是羟基或酰氧基; R 3是氢或羟基; R 4是氢或羟基; R 5是氢或羟基磺酰氧基; R 6是氢或氨基甲酰基

    PROCESS FOR THE DEACYLATION OF CYCLIC LIPOPEPTIDES
    7.
    发明公开
    PROCESS FOR THE DEACYLATION OF CYCLIC LIPOPEPTIDES 失效
    PROZESSFÜRDIE DEACYLIERUNG VON ZYKLISCHEN LIPOPEPTIDEN。

    公开(公告)号:EP0885957A1

    公开(公告)日:1998-12-23

    申请号:EP97905455.8

    申请日:1997-03-06

    IPC分类号: C12N9/80

    CPC分类号: C12R1/465 C07K7/56 C12N9/80

    摘要: There is provided a cyclic lipopeptide acylase which is capable of deacylating the acyl side chain of a cyclic lipopeptide compound, e.g. Substance FR901379 or its analog, of the following general formula [I] with effectiveness as well a method of producing a cyclic peptide compound which comprises using the acylase.


    [wherein R 1 is acyl;
    R 2 is hydroxy or acyloxy;
    R 3 is hydrogen or hydroxy;
    R 4 is hydrogen or hydroxy;
    R 5 is hydrogen or hydroxysulfonyloxy; and
    R 6 is hydrogen or carbamoyl.]

    摘要翻译: 提供了环状脂肽酰基转移酶,其能够使环状脂肽化合物的酰基侧链脱酰化,例如, 具有以下通式的物质FR901379或其类似物,其有效性以及包含使用酰基转移酶的环肽化合物的制备方法。 其中R 1是酰基; R 2是羟基或酰氧基; R 3是氢或羟基; R 4是氢或羟基; R 5是氢或羟基磺酰氧基; 和R 6是氢或氨基甲酰基