公开(公告)号：EP2438178A2

公开(公告)日：2012-04-11

申请号：EP10784212.2

申请日：2010-06-04

申请人： Genomatica, Inc.

发明人： VAN DIEN, Stephen, J. ,  BURGARD, Anthony, P. ,  HASELBECK, Robert ,  PUJOL-BAXLEY, Catherine, J. ,  NIU, Wei ,  TRAWICK, John, D. ,  YIM, Harry ,  BURK, Mark, J. ,  OSTERHOUT, Robin, E. ,  SUN, Jun

IPC分类号： C12P7/16

CPC分类号： C12P7/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52

摘要： The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.

公开(公告)号：EP2488652A1

公开(公告)日：2012-08-22

申请号：EP10824061.5

申请日：2010-10-13

申请人： Genomatica, Inc.

发明人： HASELBECK, Robert ,  TRAWICK, John, D. ,  NIU, Wei ,  BURGARD, Anthony, P.

IPC分类号： C12P7/26 ,  C12N1/20

CPC分类号： C12P7/18 ,  C12P7/24 ,  C12P7/42 ,  C12P13/001 ,  C12P19/40

摘要： The invention provides non-naturally occurring microbial organisms comprising a 1,4- butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, A- hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.

摘要翻译： 本发明提供非天然存在的微生物有机体，其包括1,4-丁二醇（BDO），4-羟基丁酰基-CoA，4-羟基丁醛或腐胺途径包含至少一种外源核酸编码BDO，4-羟基丁酰-CoA，4- -hydroxybutanal或腐胺途径酶以足够的量过表达以产生BDO，4-羟基丁酰基-CoA，4-羟基丁醛或腐胺和BDO的表达进一步优化。 本发明另外提供使用搜索的微生物以产生BDO，4-羟基丁酰基-CoA，4-羟基丁醛或腐胺的方法。