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    Processes for production of human epidermal growth factor by genetic engineering
    2.
    发明公开
    Processes for production of human epidermal growth factor by genetic engineering 失效
    Verfahren zur Herstellung von Epidermal-Wachstumsfaktor durch genetische Transformation。

    公开(公告)号：EP0335400A2

    公开(公告)日：1989-10-04

    申请号：EP89105639.2

    申请日：1989-03-30

    申请人： HITACHI, LTD. ,  Hitachi Chemical Co., Ltd.

    发明人： Shimizu, Norio ,  Harada, Yoshinori ,  Fukuzono, Shinichi ,  Fujimori, Kiyoshi

    IPC分类号： C12N15/18 ,  C07H21/04 ,  C12N1/20 ,  C12P21/02

    CPC分类号： C12N15/71 ,  C07K14/485 ,  C07K2319/02 ,  C07K2319/75

    摘要： By culturing E. coli bearing a DNA sequence comprising a tryptophan regulatory gene, a gene coding for the N-terminal of trpE or trpL polypeptide and a specific synthetic gene coding for human epidermal growth factor, hEGF can be obtained as fused protein efficiently. hEGF can also be obtained as matured protein efficiently by culturing E. coli bearing a DNA sequence comprising a tryptophan regulatory gene, SD sequence of tryptophan L or E, ATG and a specific synthetic gene coding for human epidermal growth factor immediately downstream said ATG. Thus obtained hEGF is applicable to treatment of gastric ulcer or wounds.

    摘要翻译： 通过培养含有色氨酸调节基因的DNA序列的大肠杆菌，编码trpE或trpL多肽的N末端的基因和编码人表皮生长因子的特异性合成基因，可以有效地获得作为融合蛋白的hEGF。 通过培养具有包含色氨酸调节基因，色氨酸L或E的SD序列，ATG的DNA序列和紧邻ATG下游的人表皮生长因子编码的特异性合成基因，可以有效地培养具有成熟蛋白质的成熟蛋白质。 因此获得的hEGF适用于治疗胃溃疡或创伤。

    Method for cultivation with acetate concentration monitoring and apparatus for the same
    6.
    发明公开
    Method for cultivation with acetate concentration monitoring and apparatus for the same 失效
    Verfahren zurZüchtungdurch Kontrollieren der Acetatkonzentration und Apparatdafür。

    公开(公告)号：EP0283726A1

    公开(公告)日：1988-09-28

    申请号：EP88102463.2

    申请日：1988-02-19

    申请人： HITACHI, LTD.

    发明人： Shimizu, Norio ,  Fukuzono, Shinichi ,  Fujimori, Kiyoshi ,  Nishimura, Nobuko ,  Odawara, Yoji

    IPC分类号： C12P1/00 ,  C12M1/36 ,  C12P21/02

    CPC分类号： C12M41/32 ,  C12M41/48 ,  C12P1/00 ,  C12P21/02 ,  Y10S435/849

    摘要： In a method for cultivation of microorganisms or animal cell or plant cells to produce metabolites, high density cultivation, high cell yield and high production of desired products can be achieved by performing culti­vation through monitoring acetate concentration in culture broth and regulating assimilation of acetate in the culture broth by the microorganisms or animal cell or plant cells to control the acetate concentration to a set value or less, and as an apparatus for such cultivation, preferred is an apparatus comprising a culture tank for performing cultivation, an acetate concentration analyzer in culture broth, a substrate tank for storing substrate, a substrate feeding device of variable flow type for feeding substrate and a control device for outputting a signal for controlling a substrate flow from the substrate feeding device based on a signal from the analyzer.

    摘要翻译： 在培养微生物或动物细胞或植物细胞以产生代谢物的方法中，可以通过监测培养液中的乙酸盐浓度并调节乙酸盐的同化来实现培养，从而实现高密度培养，高产量和高产量的所需产物 通过微生物或动物细胞或植物细胞培养培养液以控制乙酸盐浓度达到设定值或更低，作为这样的培养装置，优选包括用于进行培养的培养罐，培养液中的乙酸盐浓度分析仪 ，用于存储基板的基板罐，用于进给基板的可变流动型的基板供给装置和基于来自分析器的信号从基板供给装置输出用于控制基板流的信号的控制装置。