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1.发明公开
公开(公告)号：EP2765424A4
公开(公告)日：2015-03-04
申请号：EP12838806
申请日：2012-10-04
发明人： SAITO TOSHIRO , HAMASAKI KOSHIN , TAKAHASHI SATOSHI , MAESHIMA MUNEO , IMAI KYOKO , IMAI KAZUMICHI , TAO RYUJI
IPC分类号： G01N33/553 , C12M1/00 , C12Q1/68 , G01N21/64 , G01N33/543
CPC分类号： C12Q1/6834 , C12Q1/6804 , C12Q1/6837 , G01N21/6428 , G01N21/6452 , G01N33/54326 , G01N33/54346 , G01N33/54393 , G01N33/587 , G01N2021/6421 , C12Q2525/161 , C12Q2545/114 , C12Q2563/107 , C12Q2563/143 , C12Q2563/149 , C12Q2565/514 , C12Q2563/119
摘要： An object of the present invention is to provide a method and means for analyzing biomolecules that can realize, in biomolecule analysis, a wide dynamic range attained by counting the number of biomolecules and rapid analysis. The present invention relates to a method for analyzing biomolecules, comprising the steps of: immobilizing biomolecules 101 to be analyzed on surfaces of magnetic microparticles 108; reacting labeled probe molecules 104 with the biomolecules 101 to be analyzed; collecting and immobilizing the microparticles 108 on a support substrate 110; and measuring a label on the support substrate 110. Since single-molecule immobilized magnetic microparticles are used in the present invention, the number of biomolecules can be counted, and since hybridization and an antigen-antibody reaction are performed with the microparticles having biomolecules immobilized thereon dispersed, the reaction can be rapidly performed. Further, the type and the abundance of biomolecules of interest can be determined at a single molecular level, so as to evaluate, in particular, the absolute concentration of biomolecules.
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2.发明公开
公开(公告)号：EP2515121A4
公开(公告)日：2018-04-04
申请号：EP10837485
申请日：2010-12-08
发明人： UEMATSU CHIHIRO , MAESHIMA MUNEO
CPC分类号： G01N35/10 , B01L3/0275 , B01L2200/0647 , B01L2200/143 , B01L2300/0829 , B01L2400/043 , G01N35/0098 , G01N2035/1048 , Y10T436/25
摘要： This invention concerns a sample processing device capable of efficiently recovering biological molecules, such as nucleic acids or proteins. The sample processing device is capable of placing a reaction container having a plurality of reaction sites, and it comprises a nozzle mechanism with a nozzle capable of attaching and removing a dispenser tip for dispensing a solution into the reaction sites of the reaction container and a magnetic tip for generating a magnetic field that allows magnetic beads to migrate to a space among the plurality of reaction sites in the reaction container, and a drive control unit controlling the nozzle mechanism.
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公开(公告)号：EP2453224A4
公开(公告)日：2015-05-27
申请号：EP10797092
申请日：2010-07-05
CPC分类号： G01N21/51 , G01N15/0205 , G01N21/253 , G01N2015/0065 , G01N2035/00396 , G01N2035/0453
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4.发明公开
公开(公告)号：EP2639587A4
公开(公告)日：2016-11-02
申请号：EP11840550
申请日：2011-10-27
CPC分类号： B01L7/52 , B01L3/50851 , B01L3/50855 , B01L7/5255 , B01L2200/025 , B01L2300/0803 , B01L2300/0822 , G01N21/6456 , G01N21/6486 , G01N35/028 , G01N2035/00366 , G01N2035/0429 , G01N2035/0441 , G01N2035/0465
摘要： Provided is a technology such that, in nucleic acid analysis, a high degree of freedom in loading or unloading a reaction plate can be obtained and a sample can be efficiently analyzed. A reaction plate assembly includes a reaction plate with one or more reaction wells, a visible light transmissive cover mounted on the reaction plate and covering the reaction wells, and a visible light transmissive weight member covering the cover. The reaction wells are disposed in an arc shape along the circumference of a circle with a predetermined radius r1.
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公开(公告)号：EP2735618A4
公开(公告)日：2015-01-21
申请号：EP12815243
申请日：2012-05-16
发明人： SAITO TOSHIRO , HAMASAKI KOSHIN , TAKAHASHI SATOSHI , MAESHIMA MUNEO , IMAI KYOKO , IMAI KAZUMICHI , TAO RYUJI
CPC分类号： C12Q1/6834 , C12N15/1065 , C12Q2525/207 , C12Q2537/125 , C12Q2537/143 , C12Q2563/143 , C12Q2563/149
摘要： The object of the present invention is to provide a convenient method for nucleic acid analysis, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provide is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. The present invention enables nucleic acids to be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.
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公开(公告)号：EP2871460A4
公开(公告)日：2016-02-17
申请号：EP13813834
申请日：2013-06-21
发明人： TAKAHASHI MAKIKO , MAEDA KOHSHI , SHOJI YASUNORI , MAESHIMA MUNEO
CPC分类号： C12N15/1013 , C12Q1/6806 , C12Q2527/125 , C12Q2547/10 , C12Q2563/143
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7.发明公开DEVICE FOR HARVESTING BACTERIAL COLONY AND METHOD THEREFOR 有权VORRICHTUNG ZUR GEWINNUNG EINER BAKTERIENKOLONIE UND VERFAHRENDAFÜR
公开(公告)号：EP2497823A4
公开(公告)日：2015-07-29
申请号：EP10828350
申请日：2010-11-05
发明人： HONDA TOSHIFUMI , URANO YUTA , MURAKAMI SHINYA , MAEKAWA AKIRA , MAESHIMA MUNEO , ISHIDA YOSHIKO , FUJITA HIROKO
IPC分类号： C12M1/34
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