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    GEL PATTERNED SURFACES
    4.
    发明公开
    GEL PATTERNED SURFACES 审中-公开
    STRUKTURIERTEGELOBERFLÄCHEN

    公开(公告)号：EP2961524A1

    公开(公告)日：2016-01-06

    申请号：EP14710688.4

    申请日：2014-02-21

    申请人： Illumina, Inc.

    发明人： BARNARD, Steven M. ,  BOWEN, M. Shane ,  ROGERT BACIGALUPO, Maria Candelaria ,  GEORGE, Wayne N. ,  BROWN, Andrew A. ,  TSAY, James

    IPC分类号： B01J19/00 ,  C12Q1/68 ,  G01N27/447

    CPC分类号： B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00621 ,  B01J2219/00644 ,  B01J2219/00722 ,  C12N15/1068 ,  C12Q1/6837 ,  C12Q2563/159

    摘要： Provided is an array including a solid support having a surface, the surface having a plurality of wells, the wells containing a gel material, the wells being separated from each other by interstitial regions on the surface, the interstitial regions segregating the gel material in each of the wells from the gel material in other wells of the plurality; and a library of target nucleic acids in the gel material, wherein the gel material in each of the wells comprises a single species of the target nucleic acids of the library. Methods for making and using the array are also provided.

    摘要翻译： 提供了一种包括具有表面的固体支持物的表面，表面具有多个孔，孔包含凝胶材料，孔通过表面上的间隙区彼此分离，间隙区域在每个孔中分离凝胶材料 的来自多个其他孔中的凝胶材料的孔; 以及凝胶材料中的靶核酸文库，其中每个孔中的凝胶材料包含文库的单个靶核酸的种类。 还提供了制作和使用阵列的方法。

    COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FRO COMPLEX MIXTURES USING HYBRIDIZATION
    5.
    发明公开
    COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FRO COMPLEX MIXTURES USING HYBRIDIZATION 审中-公开
    组合物和方法从复杂的混合物杂交NUCLEIC代表选择

    公开(公告)号：EP2076609A1

    公开(公告)日：2009-07-08

    申请号：EP07837278.6

    申请日：2007-08-24

    申请人： Illumina Inc.

    发明人： STUELPNAGEL, John R. ,  BARKER, David L. ,  VELARDE, Jorge Jr. ,  BARNARD, Steven M. ,  GRAIGE, Michael

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1072 ,  C12Q1/6837 ,  C12Q2565/515

    摘要： The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.