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    DISPOSABLE, INTEGRATED MICROFLUIDIC CARTRIDGE AND METHODS OF MAKING THE SAME
    2.
    发明公开
    DISPOSABLE, INTEGRATED MICROFLUIDIC CARTRIDGE AND METHODS OF MAKING THE SAME 审中-公开

    公开(公告)号：EP3698874A1

    公开(公告)日：2020-08-26

    申请号：EP20165370.6

    申请日：2015-03-11

    申请人： Illumina, Inc.

    发明人： SABOUNCHI, Poorya ,  JAVANMARDI, Behnam ,  KHURANA, Tarun ,  PAIK, Philip ,  LIN, Yan-You

    IPC分类号： B01L3/00 ,  C12Q1/6806 ,  B01L7/00 ,  C12Q1/6869

    摘要： The disclosed embodiments concern microfluidic cartridges for detecting biological reactions. In some embodiments, the microfluidic cartridges are configured to perform sequencing operations on a nucleic acid sample. In one aspect, a microfluidic cartridge includes a stack of fluidics layers defining channels and valves for processing the nucleic acid sample to be sequenced, and a solid state CMOS biosensor integrated in the stack. The biosensor has an active area configured to detect signals of biological reactions, wherein substantially all of the active area is available for reagent delivery and illumination during operation. In another aspect, a microfluidic cartridge includes: (a) a flow cell including a reaction site area encompassing one or more reaction sites; (b) fluidics channels for delivering reactants to and/or removing reactants from the reaction site area; (c) a biosensor having an active area configured to detect signals of biological reactions in the reaction site area. The reaction site area is proximal to the active area of the biosensor and the reaction site area spans substantially all of the active area of the biosensor. In some embodiments, the fluidics channels do not substantially overlap with the active area of the biosensor. Methods for manufacturing and operating the microfluidic cartridges are also disclosed.

    DISPOSABLE, INTEGRATED MICROFLUIDIC CARTRIDGE AND METHODS OF MAKING AND USING SAME
    4.
    发明公开
    DISPOSABLE, INTEGRATED MICROFLUIDIC CARTRIDGE AND METHODS OF MAKING AND USING SAME 审中-公开
    一次性集成微流体盒和方法进行生产及其用途

    公开(公告)号：EP3116651A1

    公开(公告)日：2017-01-18

    申请号：EP15711627.8

    申请日：2015-03-11

    申请人： Illumina, Inc.

    发明人： SABOUNCHI, Poorya ,  JAVANMARDI, Behnam ,  KHURANA, Tarun ,  PAIK, Philip ,  LIN, Yan-You

    IPC分类号： B01L7/00 ,  B01L3/00

    摘要： The disclosed embodiments concern microfluidic cartridges for detecting biological reactions. In some embodiments, the microfluidic cartridges are configured to perform sequencing operations on a nucleic acid sample. In one aspect, a microfluidic cartridge includes a stack of fluidics layers defining channels and valves for processing the nucleic acid sample to be sequenced, and a solid state CMOS biosensor integrated in the stack. The biosensor has an active area configured to detect signals of biological reactions, wherein substantially all of the active area is available for reagent delivery and illumination during operation. In another aspect, a microfluidic cartridge includes: (a) a flow cell including a reaction site area encompassing one or more reaction sites; (b) fluidics channels for delivering reactants to and/or removing reactants from the reaction site area; (c) a biosensor having an active area configured to detect signals of biological reactions in the reaction site area. The reaction site area is proximal to the active area of the biosensor and the reaction site area spans substantially all of the active area of the biosensor. In some embodiments, the fluidics channels do not substantially overlap with the active area of the biosensor. Methods for manufacturing and operating the microfluidic cartridges are also disclosed.

    摘要翻译： 盘游离缺失实施例涉及用于检测生物反应的微流体盒。 在一些实施方案中，微流体盒被配置为对核酸样品进行测序操作。 在一个方面，微流体盒包括堆叠层流体限定通道和阀，用于处理核酸样品待测序的，以及固态CMOS生物传感器集成在所述堆栈中。 该生物传感器具有被配置为检测生物反应的信号的有效面积，基本上所有worin有源区的可用于在操作期间试剂递送和照明。 在另一个方面中，微流体盒包括：（a）一种流动池包含反应位点区域包含一个或多个反应位点; （B）流体通道用于输送反应物和/或除去反应位点区的反应物; （C）具有在被配置为检测在反应位点区域的生物反应的信号有效区域的生物传感器。 反应位点区域是靠近所述生物传感器和所述反应位点区的有源区基本上跨越所有生物传感器的活性区域。 在一些实施例中，流体通道基本上不与生物传感器的活性区域重叠。 制造和操作微流体卡盒的方法是使游离缺失盘。

    SPATIAL INDEXING OF GENETIC MATERIAL AND LIBRARY PREPARATION USING HYDROGEL BEADS AND FLOW CELLS
    5.
    发明公开
    SPATIAL INDEXING OF GENETIC MATERIAL AND LIBRARY PREPARATION USING HYDROGEL BEADS AND FLOW CELLS 审中-公开

    公开(公告)号：EP4289967A2

    公开(公告)日：2023-12-13

    申请号：EP23181622.4

    申请日：2018-07-31

    申请人： Illumina, Inc.

    发明人： KHURANA, Tarun, Kumar ,  WU, Yir-Shyuan ,  CHEN, Xi-Jun ,  GORPE-YASAR, Filiz ,  LIN, Yan-You ,  POPIC, Victoria ,  JAEGER, Erich, B. ,  RONAGHI, Mostafa

    IPC分类号： C12Q1/6869

    摘要： Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

    SPATIAL INDEXING OF GENETIC MATERIAL AND LIBRARY PREPARATION USING HYDROGEL BEADS AND FLOW CELLS
    8.
    发明公开
    SPATIAL INDEXING OF GENETIC MATERIAL AND LIBRARY PREPARATION USING HYDROGEL BEADS AND FLOW CELLS 审中-公开

    公开(公告)号：EP4446437A2

    公开(公告)日：2024-10-16

    申请号：EP24185058.5

    申请日：2018-07-31

    申请人： ILLUMINA, INC.

    发明人： KHURANA, Tarun Kumar ,  WU, Yir-Shyuan ,  CHEN, Xi-Jun ,  GORPE-YASAR, Filiz ,  LIN, Yan-You ,  POPIC, Victoria ,  JAEGER, Erich B. ,  RONAGHI, Mostafa

    IPC分类号： C12Q1/6869

    摘要： Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.